Effect of Oxygenated Perfluorocarbon on Isolated Islets During Transportation

Terai, Sachio; Tsujimura, Takuya; Li, Shiri; Hori, Yoshiaki; Toyama, Hirochika; Shinzeki, Makoto; Matsumoto, Ippei; Kuroda, Yoshikazu; Ku, Yonson

doi:10.1016/j.jss.2009.03.082

Cited by 10 publications

(7 citation statements)

References 19 publications

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“…In contrast, when the ratio of ADP/ATP was assessed in tissue, the lean-diet-fed mice demonstrated a significant decrease in comparison to high-fat-diet-fed mice (Figure 5(b)). This suggests that energy status and metabolism are the crux of the enhanced nocturnal activity, 16–18 which is consistent with this strain. Immunoblotting of the tissues from the animals showed no apparent differences in the expression of the androgen receptor between groups (Figure 5(c)).…”

Section: Resultssupporting

confidence: 73%

Diet-regulated behavior: FVB/N mice fed a lean diet exhibit increased nocturnal bouts of aggression between littermates

et al. 2019

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The hyperactive FVB/N inbred mouse strain is widely used for transgenic research applications, although rarely for behavioral studies. These mice have visual impairments via retinal degeneration, but are considered highly intelligent and rely largely on olfaction. While investigating diet-induced obesity in autotaxin transgenic FVB/N mice, we observed an increase in the necessity for male, but not female, cage separations. Based on the observations, we hypothesized that feeding FVB/N mice a lean diet increases nocturnal bouts of aggression between male littermates. The diets of adult littermates were switched from normal chow to either ad libitum high-fat (45% fat) or lean (10% fat) matched diets for 27 weeks, whereby the mice reached an average of 43 g versus 35 g, respectively. Then, cage separations due to nocturnal bouts of aggression became mandatory, even though littermates peacefully cohabitated for 10–16 weeks previously. Since the data was of an unusual nature, it required uncommon statistical methods to be engendered to evaluate whether and where significance existed. Therefore, utilizing the randomization and population models, we established a methodology and postulated that either testosterone, the autotaxin transgene or diet alteration was the causal factor. Statistical evaluation demonstrated a significant correlation between cage separations and aggressive behavior associated with the lean-diet-fed mice, not autotaxin. Biochemical data did not appear to explain the behavior. In contrast, energy metabolism highlighted differences between the groups of normally hyperactive mice by diet. This characteristic makes FVB/N male mice unsuitable subjects for long-term studies with lean-diet modifications.

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Section: Resultssupporting

confidence: 73%

Diet-regulated behavior: FVB/N mice fed a lean diet exhibit increased nocturnal bouts of aggression between littermates

et al. 2019

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“…Surprisingly, given their physical characteristics, Although our study represents only proof of principle at this stage, there are additional reasons to hope that there are only a few reports of the application of perfluorocarbons to islet culture and transportation (2,9,28,31).…”

Section: Discussionmentioning

confidence: 91%

“…Surprisingly, given their physical characteristics, there are only a few reports of the application of perfluorocarbons to islet culture and transportation (2,9,28,31). Zekorn et al first described improved islet secretory function, as gauged by a significant increase in the glucose stimulation index, using an emulsion of perfluorocarbon (FC-43) as a culture medium additive (31).…”

Section: Discussionmentioning

confidence: 99%

Use of Perfluorodecalin for Pancreatic Islet Culture Prior to Transplantation: A Liquid-Liquid Interface Culture System—Preliminary Report

et al. 2011

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Although the issue remains controversial, short-term culture is probably beneficial for islet graft quality. However, significant islet loss is invariably observed. This is related to reduced survival of large islets, which is compromised by hypoxia under standard culture conditions. We aimed to develop a method of culture, which would avoid exposure to relative hypoxia and hence maintain the quality of islets. Isolated rat islets cultured for 48 h in a liquid-liquid interface culture system (LICS) with a perfluorocarbon were compared to islets cultured under standard (C1) and suboptimal conditions (C2). Islets were tested for viability and response to a glucose challenge, and a marginal mass was transplanted into syngeneic diabetic recipients. The viability of islets after 24-h culture in LICS was higher than in C1 and C2 groups (89.0% vs. 77.5% and 64.6%, respectively) and decreased with time to reach 79.0%, 62.9%, and 53.4% after 72-h culture. The stimulation index in LICS-cultured islets was also significantly higher than in C1 and C2 groups (12.3 ± 0.4 vs. 5.8 ± 0.5 and 4.1 ± 0.2, respectively). Following transplantation of LICS-cultured islets 50% of recipients were rendered normoglycemic compared with 14.3% and 31.3% for C2 and fresh islets, respectively. Our liquid-liquid interface culture system using perfluorodecalin provides optimized culture conditions, which preserve both islet viability and their ability to engraft successfully after intraportal transplantation and could be used for islet transportation.

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“…The form of liquid PFC-based emulsions depends on the way of its bioengineering application. Two main lines of research are being actively pursued: (i) PFC-inwater emulsions for in vivo intravascular oxygen delivery (known as perfluorinated temporary blood substitutes) (Krafft et al, 2003;Riess, 2006b;Vasquez et al, 2013), as storage-media during organ transplantation procedures (Bezinover et al, 2014;Maillard et al, 2008;Terai et al, 2010), currently under development for treatment of traumatic brain and spinal cord injury (Spiess, 2009) as well as in decompression sickness therapy (Randsoe and Hyldegaard, 2009), and (ii) water-in-PFC reverse emulsions for pulmonary drug delivery (Courrier et al, 2004;Smoła et al, 2008), targeted emulsions for diagnosis and therapy (Kaufmann and Lindner, 2007;Lanza and Wickline, 2001;Martin and Dayton, 2013), as well as valuable research tool used in polymerisation technology (Chen et al, 2010;Zetterlund et al, 2008).…”

Section: Liquid Pfc-based Emulsionsmentioning

confidence: 99%

Liquid Perfluorochemicals as Flexible and Efficient Gas Carriers Applied in Bioprocess Engineering: An Updated Overview and Future Prospects

Pilarek¹

2014

Chemical and Process Engineering

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Fully synthetic, biochemically inert and water-immiscible liquid perfluorochemicals (PFCs) are recognised as flexible liquid carriers/scavengers of gaseous compounds (respiratory gases mainly, i.e. O 2 and CO 2 ) and increasingly applied in bioprocess engineering. A range of unmatched physicochemical properties of liquid PFCs, i.e. outstanding chemo-and thermostability, extremely low surface tension, simultaneous hydro-and lipophobicity, which result from carbon chain substitution with fluorine atoms (the most electronegative chemical element) and the presence of intramolecular C-F bonds (the strongest single bond known in organic chemistry) have been described in detail. Exceptional propensity to solubility of respiratory gases in liquid perfluorinated compounds has been widely discussed. Advantages and disadvantages of bioprocess applications of liquid PFCs in the form of a pure PFC as well as in an emulsified form have been pointed out. A liquid PFC-mediated mass transfer intensification in various types of microbial, plant cell and animal cell culture systems: from miniaturised microlitre-scale cultures, via biomaterial-based scaffolds containing culture systems, to litre-scale bioreactors, has been reviewed and elaborated on bearing in mind the benefits of bioprocesses.

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Effect of Oxygenated Perfluorocarbon on Isolated Islets During Transportation

Cited by 10 publications

References 19 publications

Diet-regulated behavior: FVB/N mice fed a lean diet exhibit increased nocturnal bouts of aggression between littermates

Diet-regulated behavior: FVB/N mice fed a lean diet exhibit increased nocturnal bouts of aggression between littermates

Use of Perfluorodecalin for Pancreatic Islet Culture Prior to Transplantation: A Liquid-Liquid Interface Culture System—Preliminary Report

Liquid Perfluorochemicals as Flexible and Efficient Gas Carriers Applied in Bioprocess Engineering: An Updated Overview and Future Prospects

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