Effect of P‐body component Mov10 on HCV virus production and infectivity

Liu, Dandan; Ndongwe, Tanyaradzwa P.; Puray-Chavez, Maritza; Casey, Mary C.; Izumi, Tatsuro; Pathak, Vinay K.; Tedbury, Philip R.; Sarafianos, Stefan G.

doi:10.1096/fj.201800641r

Cited by 13 publications

(12 citation statements)

References 67 publications

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“…2B ). This result was in line with other described MOV10 interactions that were also RNA dependent ( 43 , 44 , 48 , 49 ). These data demonstrated that MOV10 interacted with MERS-CoV N protein in an RNA-dependent manner.…”

Section: Resultssupporting

confidence: 92%

“…MOV10 antiviral activity during MERS-CoV infection was dependent on its helicase activity. This was in contrast to the absence of a MOV10 helicase activity requirement for antiviral activity during HIV-1, influenza virus, HCV, or bunyavirus infections ( 40 , 43 , 44 , 64 , 65 ), strongly suggesting that MOV10 can exert its antiviral function by different mechanisms in different RNA viruses. Interestingly, MERS-CoV N protein was present in MOV10 complexes formed by the MOV10 mutant without helicase activity, suggesting that CoV N protein presence in MOV10 RNPs is not enough to promote its antiviral activity.…”

Section: Discussionmentioning

confidence: 79%

“…Both proviral and antiviral functions have been reported for MOV10. It is required for hepatitis delta virus (HDV) replication but not for the translation of its mRNA ( 37 ), facilitates enterovirus replication ( 38 ), inhibits replication of HIV-1 and other retroviruses at multiple steps ( 39 ), inhibits nuclear import of influenza virus nucleoprotein ( 40 ), inhibits porcine reproductive and respiratory syndrome virus (PRRSV) replication by avoiding nucleocapsid protein trafficking to the nucleus ( 41 ), inhibits hepatitis C virus (HCV) and dengue virus replication by partly unknown mechanisms ( 42 , 43 ), and inhibits bunyavirus replication by blocking several nucleoprotein functions ( 44 ). In the case of hepatitis B virus (HBV), contradictory data have been reported for both its proviral ( 45 ) and antiviral ( 46 ) activities.…”

Section: Introductionmentioning

confidence: 99%

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MOV10 Helicase Interacts with Coronavirus Nucleocapsid Protein and Has Antiviral Activity

Wang

Sola

Enjuanes

et al. 2021

mBio

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Section: Resultssupporting

confidence: 92%

Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

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MOV10 Helicase Interacts with Coronavirus Nucleocapsid Protein and Has Antiviral Activity

Wang

Sola

Enjuanes

et al. 2021

mBio

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“…Overexpression of MOV10 also inhibits replication and reduces infectivity of a wide range of exogenous retroviruses, including human immunodeficiency virus type-1 (HIV-1) [35,[42][43][44][45]. Furthermore, MOV10 is an interferon-stimulated gene [46,47], which exhibits broad antiviral activity [46,[48][49][50][51][52][53][54][55]. Apart from retrotransposon restricting and antiviral activities, MOV10 has an essential role in post-transcriptional gene regulation, especially in the microRNA (miRNA) pathway [56][57][58][59].…”

Section: Introductionmentioning

confidence: 99%

CRL4-DCAF12 Ubiquitin Ligase Controls MOV10 RNA Helicase during Spermatogenesis and T Cell Activation

Liďák

Baloghová

Kor̆ínek

et al. 2021

IJMS

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Multisubunit cullin-RING ubiquitin ligase 4 (CRL4)-DCAF12 recognizes the C-terminal degron containing acidic amino acid residues. However, its physiological roles and substrates are largely unknown. Purification of CRL4-DCAF12 complexes revealed a wide range of potential substrates, including MOV10, an “ancient” RNA-induced silencing complex (RISC) complex RNA helicase. We show that DCAF12 controls the MOV10 protein level via its C-terminal motif in a proteasome- and CRL-dependent manner. Next, we generated Dcaf12 knockout mice and demonstrated that the DCAF12-mediated degradation of MOV10 is conserved in mice and humans. Detailed analysis of Dcaf12-deficient mice revealed that their testes produce fewer mature sperms, phenotype accompanied by elevated MOV10 and imbalance in meiotic markers SCP3 and γ-H2AX. Additionally, the percentages of splenic CD4+ T and natural killer T (NKT) cell populations were significantly altered. In vitro, activated Dcaf12-deficient T cells displayed inappropriately stabilized MOV10 and increased levels of activated caspases. In summary, we identified MOV10 as a novel substrate of CRL4-DCAF12 and demonstrated the biological relevance of the DCAF12-MOV10 pathway in spermatogenesis and T cell activation.

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“…MOV10 has anti-HCV activity similar to that against HIV-1 and HBV (D. Liu et al, 2020). Liu and colleagues showed that MOV10 localization in the cytoplasm changes from scattered to foci upon HCV infection.…”

Section: Hepatitis C Virusmentioning

confidence: 99%

Unwinding the roles ofRNAhelicaseMOV10

et al. 2021

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MOV10 is an RNA helicase that associates with the RNA-induced silencing complex component Argonaute (AGO), likely resolving RNA secondary structures. MOV10 also binds the Fragile X mental retardation protein to block AGO2 binding at some sites and associates with UPF1, a principal component of the nonsense-mediated RNA decay pathway. MOV10 is widely expressed and has a key role in the cellular response to viral infection and in suppressing retrotransposition. Posttranslational modifications of MOV10 include ubiquitination, which leads to stimulation-dependent degradation, and phosphorylation, which has an unknown function. MOV10 localizes to the nucleus and/or cytoplasm in a cell type-specific and developmental stage-specific manner. Knockout of Mov10 leads to embryonic lethality, underscoring an important role in development where it is required for the completion of gastrulation. MOV10 is expressed throughout the organism; however, most studies have focused on germline cells and neurons. In the testes, the knockdown of Mov10 disrupts proliferation of spermatogonial progenitor cells. In brain, MOV10 is significantly elevated postnatally and binds mRNAs encoding cytoskeleton and neuron projection proteins, suggesting an important role in neuronal architecture. Heterozygous Mov10 mutant mice are hyperactive and anxious and their cultured hippocampal neurons have reduced dendritic arborization. Zygotic knockdown of Mov10 in Xenopus laevis causes abnormal head and eye development and mislocalization of neuronal precursors in the brain. Thus, MOV10 plays a vital role during development, defense against viral infection and in neuronal development and function: its many roles and regulation are only beginning to be unraveled.

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Effect of P‐body component Mov10 on HCV virus production and infectivity

Cited by 13 publications

References 67 publications

MOV10 Helicase Interacts with Coronavirus Nucleocapsid Protein and Has Antiviral Activity

MOV10 Helicase Interacts with Coronavirus Nucleocapsid Protein and Has Antiviral Activity

CRL4-DCAF12 Ubiquitin Ligase Controls MOV10 RNA Helicase during Spermatogenesis and T Cell Activation

Unwinding the roles ofRNAhelicaseMOV10

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