Effect of P38 MAPK on the apoptosis of human lung adenocarcinoma cell induced by the spider venom

Abstract

Background: The venom of spiders may inspire new drugs to treat carcinoma. The aim of this study was to investigate the effect of P38 MAPK on tumor necrosis factorrelated apoptosis of lung adenocarcinoma cell line by spider venom. Methods: Lung adenocarcinoma A549 cells were cultured in PRMI-1640 medium and the apoptosis rate was observed after treatment with spider venom with a flow cytometer. The expression of P38 MAPK protein in lung cancer cells was analyzed using Western blot. Results: After targeting spider venom intervention, the expression of P38 MAPK in the cells compared to the control group was significantly down-regulated. The anticancer potency of spider venom is associated with decreased expression of P38 MAPK of lung adenocarcinoma cancer cells.

Anti-proliferative Effects ofAndroctonus amoreuxiScorpion andCerastes cerastesSnake Venoms on Human Prostate Cancer Cells

“…Our results show that a 24 hours treatment of PC3 cells with the IC 50 of these venoms caused a significant increase in the activity of the antioxidant enzymes in the cell lysate compared to the control cells (Table 1). This agrees with reports that showed an increase in lipid peroxidation level and antioxidant enzymes when using venom as a treatment 28,29. In addition, da Silva et al7 reported increased activities of CAT and GST in venom-injected experimental animals, which also agrees with our results.…”

Effect of spider venom on inhibition proliferation of TE13 cells in vivo and in vitro

“…Apoptosis, necrosis, and lysis of tumor cells are possible mechanisms by which the drug inhibited tumor growth. In vitro and in vivo assays on human cervical carcinoma cell line (HeLa), 15 hepatocellular carcinoma cell line (BEL-7402), 16 and human lung adenocarcinoma 17 suggested that SV facilitates apoptosis and functions as an effective anticancer agent. Further, more structural aspects responsible for these activities need to be defined, and the results of more advanced in vivo tests will be pivotal in contemplating SVs further development as a clinically useful pharmaceutical agent.…”

Phlogiellus bundokalbo spider venom: cytotoxic fractions against human lung adenocarcinoma (A549) cells

“…The cells were washed with PBS and 50(L of sodium 3’-[1-phenylamino-carbonyl)-3,4-tetrazolium]-bis (4-methoxy-6-nitro) benzene-sulfonic acid hydrate (MTT) at 1 mg/mL concentration was added to each well. The cells were incubated for 4 hours before the addition of 100 µL dimethyl sulfoxide and absorbance was read at 570nm using Multiskan GO Microplate Spectrophotometer (Thermo Scientific, USA) [ 9 ]. Average triplicate readings and blanks were utilized to determine the inhibition rate.…”