Effect of Palmitoyl-Pentapeptide (Pal-KTTKS) on Wound Contractile Process in Relation with Connective Tissue Growth Factor and α-Smooth Muscle Actin Expression

Park, Hyun-Ju; An, Eun-Jin; Lee, Ae‐Ri Cho

doi:10.1007/s13770-016-0017-y

Cited by 11 publications

(8 citation statements)

References 27 publications

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“…In view of the above, we hypothesized that the conjugation of a collagenesis-inducing, or collagen-boosting peptide (CBP) to an AMP known to be active against both Gram-positive and Gram-negative bacteria might produce dual-action peptide chimeras retaining the properties of their parent CBP and AMP motifs, hence, with potential interest for the management of cSSTI. To this end, the CBP chosen was the matrikine-like peptide KTTKS, also known as “pentapeptide-4” (ahead abbreviated to PP4) whose palmitoylated form (ahead abbreviated to C 16 -PP4) is widely used as a cosmeceutical known as “Matrixyl” (Jones et al, 2013; Choi et al, 2014; Aldag et al, 2016; Schagen, 2017) and reported to possess tissue regenerative properties (Tsai et al, 2007; Park et al, 2017; Krishnamoorthy et al, 2018). As the AMP motif, we chose a previously de novo designed synthetic peptide, named 3.1, earlier reported as highly active against both Gram-positive and Gram-negative bacteria (Kang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Turning a Collagenesis-Inducing Peptide Into a Potent Antibacterial and Antibiofilm Agent Against Multidrug-Resistant Gram-Negative Bacteria

et al. 2019

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Antimicrobial resistance is becoming one the most serious health threats worldwide, as it not only hampers effective treatment of infectious diseases using current antibiotics, but also increases the risks of medical procedures like surgery, transplantation, bone and dental implantation, chemotherapy, or chronic wound management. To date, there are no effective measures to tackle life-threatening nosocomial infections caused by multidrug resistant bacterial species, of which Gram-negative species within the so-called “ESKAPE” pathogens are the most worrisome. Many such bacteria are frequently isolated from severely infected skin lesions such as diabetic foot ulcers (DFU). In this connection, we are pursuing new peptide constructs encompassing antimicrobial and collagenesis-inducing motifs, to tackle skin and soft tissue infections by exerting a dual effect: antimicrobial protection and faster healing of the wound. This produced peptide 3.1-PP4 showed MIC values as low as 1.0 and 2.1 μM against Escherichia coli and Pseudomonas aeruginosa , respectively, and low toxicity to HFF-1 human fibroblasts. Remarkably, the peptide was also potent against multidrug-resistant isolates of Klebsiella pneumoniae, E. coli, and P. aeruginosa (MIC values between 0.5 and 4.1 μM), and hampered the formation of/disaggregated K. pneumoniae biofilms of resistant clinical isolates. Moreover, this notable hybrid peptide retained the collagenesis-inducing behavior of the reference cosmeceutical peptide C 16 -PP4 (“Matrixyl”). In conclusion, 3.1-PP4 is a highly promising lead toward development of a topical treatment for severely infected skin injuries.

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Section: Introductionmentioning

confidence: 99%

Turning a Collagenesis-Inducing Peptide Into a Potent Antibacterial and Antibiofilm Agent Against Multidrug-Resistant Gram-Negative Bacteria

et al. 2019

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“…(ii) negative vesicles might increase the retention of encapsulated lipophilic molecules in the skin, and the improvement of drug retention in negative vesicles might be related to the ability of these vesicles to promote structural changes in the deep layer of the skin, facilitating drug retention into the skin (Ogiso et al., 2001 ; Maione-Silva et al., 2019 ). (iii) as a signal peptide in skin tissue, KTTKS could be recognized and bound by signal recognition particles (SRP) in skin tissues, resulting in the retention of KTTKS modified ethosomes in the skin (Tang et al., 2013 ; Park et al., 2017 ). In conclusion, IMC-KTTKS-Es gel could increase the intradermal retention of drugs and reduce the number of drugs entering the systemic circulation, and these characteristics are expected to increase the curative effect and reduce the risk of increasing systemic toxicity when used in the treatment of local skin diseases.…”

Section: Resultsmentioning

confidence: 99%

Pentapeptide modified ethosomes for enhanced skin retention and topical efficacy activity of indomethacin

Niu

Yuan

et al. 2022

Drug Delivery

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Challenges associated with topical analgesics and anti-inflammatory drugs include poor drug penetration and retention at the desired lesion site. Therefore, improving these challenges would help to reduce the toxic and side effects caused by drug absorption into the systemic circulation and improve the therapeutic efficacy of topical therapeutic drugs. Pentapeptide (KTTKS) is a signal peptide in skin tissue, it can be recognized and bound by signal recognition particles. In the current study, we successfully prepared novel indomethacin (IMC) loaded KTTKS-modified ethosomes (IMC-KTTKS-Es), and the physicochemical properties and topical efficacy were investigated. Results showed that the prepared IMC-KTTKS-Es displayed a particle size of about 244 nm, a negative charge, good deformability, and encapsulation efficiency (EE) exceeding 80% for IMC, with a sustained release pattern. In vitro percutaneous permeation studies revealed that the skin retention was increased after the drug was loaded in the IMC-KTTKS-Es. Confocal laser scanning microscopy also showed improved skin retention of IMC-KTTKS-Es. In addition, IMC-KTTKS-Es showed improved topical analgesic and anti-inflammatory activity with no potentially hazardous skin irritation. This study suggested that the IMC-KTTKS-Es might be an effective drug carrier for topical skin therapy with a good safety profile.

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“…Therefore, it was suggested to consider palmitoyl-pentapeptide as a therapeutic agent in the prevention of scar lesions. 13 …”

Section: Introductionmentioning

confidence: 99%

Matrixyl Patch vs Matrixyl Cream: A Comparative In Vivo Investigation of Matrixyl (MTI) Effect on Wound Healing

et al. 2022

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Wound healing is one of the most complex biological processes. Studies show that Matrixyl (MTI), known as a cosmetic peptide, can lead to a faster healing process. The contribution of MTI to collagen formation during wound healing also depends on its mode of delivery and its release over time. Here, we investigate two modes of MTI-delivery system, the influence of MTI patch for wound healing application in comparison with MTI cream. In this study, animals were randomly divided into seven groups and studied for 21 days: patches containing two different concentrations of MTI (P-MTI-0.1 mg and P-MTI-1 mg), a cream containing MTI (C-MTI-1 mg), a patch (P-MTI-0), a cream with no MTI (C-MTI-0), a positive control (Comfeel), and a negative control (sham) group. To study the wound healing process, the change in collagen density, angiogenesis, epitheliogenesis, histopathology, immunohistochemical analysis, and wound area through imaging was monitored and measured. The macroscopic results showed that wound healing was improved from 63.5 up to 81.81% in treatment groups compared to that in the negative control group ( P < 0.05 and P < 0.001). In addition, C-MTI-1 and P-MTI-1 had a larger impact on wound healing compared to that in the positive control group (Comfeel, P < 0.05). In hematoxylin and eosin (H&E) staining analysis, the rejuvenation of skin appendage was visible in both groups of cream and patches with MTI. According to the obtained results, the re-epithelialization had a higher range for the patch with MTI in comparison with cream containing MTI and positive control.

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Effect of Palmitoyl-Pentapeptide (Pal-KTTKS) on Wound Contractile Process in Relation with Connective Tissue Growth Factor and α-Smooth Muscle Actin Expression

Cited by 11 publications

References 27 publications

Turning a Collagenesis-Inducing Peptide Into a Potent Antibacterial and Antibiofilm Agent Against Multidrug-Resistant Gram-Negative Bacteria

Turning a Collagenesis-Inducing Peptide Into a Potent Antibacterial and Antibiofilm Agent Against Multidrug-Resistant Gram-Negative Bacteria

Pentapeptide modified ethosomes for enhanced skin retention and topical efficacy activity of indomethacin

Matrixyl Patch vs Matrixyl Cream: A Comparative In Vivo Investigation of Matrixyl (MTI) Effect on Wound Healing

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