Program Studi Magister Ilmu Ternak, Fakultas Peternakan Universitas Brawijaya Jl. Veteran, Malang (65145) ABSTRAK: Inseminasi buatan (IB) menggunakan semen sexing bertujuan untuk mendapatkan pedat dengan jenis kelamin sesuai harapan. Tujuan penelitian ini bertujuan untuk mengetahui keberhasil IB semen beku non sexing dan sexing. Materi dalam penelitian menggunakan 77 ekor akseptor IB non sexing dan 77 ekor akseptor IB sexing yang di IB secara double dosis. Metode penelitian ini menggunakan eksperimental dengan percobaan lapang (field experiment) dengan perlakuan penelitian adalah P0 = straw semen beku non sexing dan P1 = straw semen beku spermatozoa Y hasil sexing dengan metode pemisahan Sentrifugasi Gradien Densitas Percoll (SGDP) yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari. Variabel penelitian meliputi Non Return Rate (NRR), Service Per Conception (S/C) dan Conception Rate (CR). Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian pada masingmasing perlakuan IB non sexing dan sexing secara berturut turut yaitu persentase nilai NRR 1 sebesar 90,91 % dan 77,92 %, nilai NRR 2 sebesar 88,31 % dan 76,62 %, persentase kebuntingan sebesar 84,42 % dan 75,33 %, nilai CR sebesar 79,22 % dan 57,14 %. Kesimpulan penelitian bahwa IB menggunakan semen beku non sexing memiliki tingkat keberhasilan yang lebih tinggi dibandingkan dengan IB menggunakan semen beku hasil sexing. Kata kunci: Inseminasi buatan; kebuntingan; double dosis.ABSTRACT: The purpose of artificial insemination using sexing semen was to obtain the calf according to the expected sex. This study aimed to determine the success of artificial insemination in non-sexing and sexing of frozen semen. The materials used in this research were 77 non-sexing artificial insemination acceptor cows and 77 sexing artificial insemination acceptor cows with double dose treatment. The method used in this study was experimental field experiment with the research treatment: P0 = frozen non-sexing semen straw and P1 = frozen semen of spermatozoa Y by sexing method using Percoll Density Gradient Centrifugation (SGDP) produced by Balai Besar Inseminasi Buatan (BBIB) Singosari. The variables of this study were Non Return Rate (NRR), Service Per Conception (S/C) and Conception Rate (CR). The data obtained were analyzed descriptively. The results of the study in each non-sexing and sexing artificial insemination treatment were: the percentage of NRR 1 were 90.91% and 77.92%, followed by NRR 2 with 88.31% and 76.62%, the pregnancy percentage were 84.42% and 75.33%, and the CR values were 79.22% and 57.14%. The conclusion of this study was artificial insemination using frozen non-sexing semen had a higher success rate compared to artificial insemination using frozen sexing semen.