Effect of pathological fracture on limb salvage surgery with preservation of the epiphysis in children with osteosarcoma of the distal femur: Two case reports

Zheng, Keke; Yu, Xiuchun; Chang, Zhengqi; Xu, Songfeng; Xu, Ming

doi:10.3892/mco.2016.777

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…The rate of incidence of osteosarcoma is higher in males than females. In general, among sick patients, out of every three people two are male and one is female [2]. From a statistical point of view, although, the disease is mainly seen in children and adolescents, it can actually occur in every age group [3].…”

Section: Introductionmentioning

confidence: 99%

Study on the efficacy of neoadjuvant chemotherapy on limb salvage surgery for osteosarcoma

Zhao¹

2018

biomedicalresearch

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In this study, 32 osteosarcoma patients admitted in the department of orthopedics from 2008 to 2016 were selected as subjects. Percutaneous intra-arterial chemotherapy was performed on 17 patients on the day before admission, and then the general chemotherapy was carried out on next day. After limb salvage surgery, the previous chemotherapy was repeated for 4 times. Before limb salvage surgery, the remaining 15 patients were treated with intravenous systemic chemotherapy. Fourteen days later, amputation was performed. Both the treatment strategies were compared and it was concluded that patients can adapt better to the method of limb salvage treatment after arterial and venous chemotherapy, thus the application of which is more promising.

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Section: Introductionmentioning

confidence: 99%

Study on the efficacy of neoadjuvant chemotherapy on limb salvage surgery for osteosarcoma

Zhao¹

2018

biomedicalresearch

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“…With the rise and development of chemotherapy drugs, cisplatin (CDDP), methotrexate and doxorubicin, have significant treatment effects on osteosarcoma, effectively improving the treatment level and cure rate of osteosarcoma ( 5 , 6 ). The application of chemotherapy drugs is promising in the treatment of osteosarcoma, but the cure rate of osteosarcoma is generally about 60% due to chemotherapy drug resistance ( 7 ). Chemotherapy drug resistance is generally divided into endogenous drug resistance and acquired drug resistance, and there are various drug resistance-related molecular mechanisms, mainly including the increase of drug efflux, and changes in targets of drug metabolism and mutant drug therapy ( 8 ).…”

Section: Introductionmentioning

confidence: 99%

Correlation between chemotherapy resistance in osteosarcoma patients and PAK5 and Ezrin gene expression

Liu

Zhou

2017

Oncol Lett

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The correlation between PAK5 (P21-activated kinase 5) and Ezrin gene expression and chemotherapy resistance of osteosarcoma patients was investigated. The cisplatin (CDDP)-resistance model of osteosarcoma cells SOSP-9607/CDDP was established to detect the cell growth curve. Methyl thiazolyl tetrazolium (MTT) assay was used to detect the drug resistance of cells to chemotherapy drugs. Transwell assay was used to detect the invasive capacity of cells. Semi-quantitative PCR (qPCR) was used to detect the mRNA expression levels in the drug resistance-related genes PAK5 and Ezrin. Western blot analysis was used to detect the protein expression levels in PAK5 and Ezrin. Tumor tissues were taken from the osteosarcoma patients with chemotherapy resistance to detect the expression levels of PAK5 and Ezrin via immunohistochemical detection, and the correlation between PAK5 and Ezrin expressions was studied. The results of MTT assay showed that the growth rate of SOSP-9607 was similar to that of SOSP-9607/CDDP, and the difference was not statistically significant (P>0.05). The sensitivity of SOSP-9607 to CDDP was significantly higher than that of SOSP-9607/CDDP, and the difference was statistically significant (P<0.01). Transwell assay showed that the migration capacity of SOSP-9607/CDDP was significantly better than that of SOSP-9607 (P<0.01), indicating that the drug resistance cell lines of osteosarcoma were constructed successfully. Semi-qPCR and western blot analysis showed that the protein expression levels in PAK5 and Ezrin in SOSP-9607/CDDP were significantly higher than those in SOSP-9607 (P<0.01). The results of immunohistochemistry showed that the expression quantities of PAK5 and Ezrin in osteosarcoma tissues were significantly higher than those in para-tumor tissues (P<0.01). Pearson's correlation analysis showed that expression of PAK5 and Ezrin was positively correlated (r=0.197, P=0.023). The osteosarcoma resistance is closely related to the expression levels of PAK5 and Ezrin genes. Thus, PAK5 and Ezrin genes may affect the tolerance of osteosarcoma patients to chemotherapy drugs during treatment via the synergistic effect.

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“…Osteosarcoma, a primary tumor disease, is also most commonly founded in the distal femur that is the anatomic site associated with maximum growth velocity with abundant blood supply [82].…”

Section: Discussionmentioning

confidence: 99%

Estudo sobre as respostas inflamatórias em modelo experimental de artrite séptica induzida por Staphylococcus aureus e suas vesículas

Fatima¹

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I would like to express my profound gratitude to all those who helped me in different ways during my PhD. In particular I am rather indebted to thank; Luciano Neder: PhD supervisor, for giving me the opportunity to work in his group. His kind supervision, guidance, and allowing me to work at my own pace and comfort made this submission possible. The biggest appreciation is the opportunity he gave me to have a work-life balance, allowing me to take decision that are suitable for my scenarios that allowed me complete mental strength and peace to fulfil my work efficiently. I will ever be thankful for all this. Tao Jin: my co-supervisor, who proved to be one of the productive additions in my scientific career. It was a great experience being at Gothenburg and exploring new areas of research in his lab at Sahlgrenska Academy. He provided me with great lab facilities along with complete independence and confidence to work independently on new projects. His friendly way of mentoring will always leave a mark on my future research career. Alfredo Ribeiro da Silva and other members of the postgraduate program of department of pathology: for making things easy going, documentations and related matters at the department. Many thanks to my lab colleagues both in Brazil and Sweden; Bruna Zanetti, Niele Dias Mendes, Renata Silvestre, Nathalia and Isabela, Deise, Anabelle, Abukar, Manli, Fei and Majd for their pleasant company and assistance during my research work as well as facilitating my stay in the lab. Camila Zambonini: Thank you so much for administrative help during all my time at this department. It was all hard to complete PhD. I also thank Felipe Denipotte Coelho, Rodrigo Banzi, Rosangela, Neide Terezinha and Antonio de Padua for helping me in several ways. Yes, that IT expert guy: every time appears with a whistle and solves the technical issues. Finally, my deepest gratitude goes to my parents for unconditional love and support throughout my life. Their hard work and dedication made me able to achieve what I seek today. Unfortunately they left too soon to see that there efforts no doubt made me stand where I am today. Their uncountable prayers have, and will always lighten my paths. May they rest in the highest place of Jannah (Heaven). Ameen. Muhammad Umer, my only brother, my first friend and my mentor and role model in every aspect of life. The space you left can never be filled. My sisters Nadia Haroon, Aisha Jaudat and Madiha Aslam for the continous support, friendly chats and unconditional love, my brother in laws Haroon Jamil, Jaudat Fayyazi and Shehzad Aslam for their prayers, wishes and support and my little group of loud and fun gang, my nephews Abdur rehman Haroon, Rayyan Aslam, Fahad Haroon and Muhammad Azlan and our lovely princess Aidha Jaudat for the fun times, treats and lovely bonding. Moreover, I extend special thanks to my in-laws for their continuous prayers and wishes for my success in life. May Allah keep my whole family and relatives, healthy and place them among His loved ones. Ameen Last bu...

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Effect of pathological fracture on limb salvage surgery with preservation of the epiphysis in children with osteosarcoma of the distal femur: Two case reports

Cited by 3 publications

References 19 publications

Study on the efficacy of neoadjuvant chemotherapy on limb salvage surgery for osteosarcoma

Study on the efficacy of neoadjuvant chemotherapy on limb salvage surgery for osteosarcoma

Correlation between chemotherapy resistance in osteosarcoma patients and PAK5 and Ezrin gene expression

Estudo sobre as respostas inflamatórias em modelo experimental de artrite séptica induzida por Staphylococcus aureus e suas vesículas

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