Effect of Pectin, a Soluble Dietary Fiber, on Functional and Morphological Parameters of the Small Intestine in Rats

Chun, Woo Young; Bamba, Tadao; Hosoda, Saichi

doi:10.1159/000199821

Cited by 43 publications

(18 citation statements)

References 27 publications

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“…These findings are consistent with the studies of Chun et al (1989) and Lizardo et al (1997) who reported an increase in brush border enzyme activity with increasing levels of soluble fiber in rats and pigs, respectively.…”

Section: Discussionsupporting

confidence: 93%

Neutral detergent-soluble fiber improves gut barrier function in twenty-five-day-old weaned rabbits1

Gómez-Conde

García

Chamorro

et al. 2007

Journal of Animal Science

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The effect of neutral detergent-soluble fiber level on gut barrier function and intestinal microbiota was examined in weaned rabbits. A control diet (AH) containing 103 g of neutral detergent-soluble fiber/ kg of DM included alfalfa hay as main source of fiber. Another diet (B-AP) was formulated by replacing half of the alfalfa hay with a mixture of beet and apple pulp resulting in 131 g of soluble fiber/kg of DM. A third diet (OH) was obtained by substituting half of the alfalfa hay with a mix of oat hulls and a soybean protein concentrate and contained 79 g of soluble fiber/kg of DM. Rabbits weaned at 25 d and slaughtered at 35 d were used to determine ileal digestibility, jejunal morphology, sucrase activity, lamina propria lymphocytes, and intestinal microbiota. Suckling 35-d-old rabbits were used to assess mucosa morphology. Mortality (from weaning to 63 d of age) was also determined. Villous height of the jejunal mucosa increased with soluble fiber (P = 0.001). Rabbits fed with the greatest level of soluble fiber (BA-P diet) showed the highest villous height/ crypt depth ratio (8.14; P = 0.001), sucrase specific activity (8,671 mumol of glucose/g of protein; P = 0.019), and the greatest ileal starch digestibility (96.8%; P = 0.002). The opposite effects were observed in rabbits fed decreased levels of soluble fiber (AH and OH diets; 4.70, 5,848 mumol of glucose/g of protein, as average, respectively). The lowest ileal starch digestibility was detected for animals fed OH diet (93.2%). Suckling rabbits of the same age showed a lower villous height/crypt depth ratio (6.70) compared with the B-AP diet group, but this ratio was higher than the AH or OH diet groups. Lower levels of soluble fiber tended (P = 0.074) to increase the cellular immune response (CD8+ lymphocytes). Diet affected IL-2 production (CD25+, P = 0.029; CD5+CD25+, P = 0.057), with no clear relationship between soluble fiber and IL-2. The intestinal microbiota biodiversity was not affected by diets (P >/= 0.38). Rabbits fed the B-AP and AH diets had a reduced cecal frequency of detection compatible with Campylobacter spp. (20.3 vs. 37.8, P = 0.074), and Clostridium perfringens (4.3 vs. 17.6%, P = 0.047), compared with the OH diet group. Moreover, the mortality rates decreased from 14.4 (OH diet) to 5.1% (B-AP diet) with the increased presence of soluble fiber in the diet. In conclusion, increased levels of dietary soluble fiber improve mucosal integrity and functionality.

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Section: Discussionsupporting

confidence: 93%

Neutral detergent-soluble fiber improves gut barrier function in twenty-five-day-old weaned rabbits1

Gómez-Conde

García

Chamorro

et al. 2007

Journal of Animal Science

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“…This is in line with previous studies showing significantly reduced small intestinal AP activity after addition of soluble dietary fibers (pectin and guar gum) to the rat diet (4,12). In contrast, another rat study using dietary pectin reported elevated ileal specific AP activity (6). At the present time, the available data on the impact of pectin on somatic, feeding and intestinal parameters are in some cases controversial.…”

Section: Discussionsupporting

confidence: 89%

“…The impact of pectin supplementation on intestinal enzyme activity is inconsistent. Some animal studies have revealed that in pectin-fed rats the specific AP activity was significantly reduced in the upper jejunum in comparison with rats fed with basal diets (4); other results indicate that pectin stimulates ileal mucosal specific activities of AP, maltase and sucrase (6). At the present time, however, information concerning the relation between pectin feeding, intestinal enzyme activity and obesity development is lacking.…”

Section: Introductionmentioning

confidence: 90%

Effect of pectin feeding on obesity development and duodenal alkaline phosphatase activity in Sprague-Dawley rats fed with high-fat/high-energy diet

Šefčíková

Racek

2016

Acta Physiol Hung

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Purpose: The objective of this study was to evaluate whether pectin feeding would affect the small intestinal function and whether these changes would lead to obesity prevention in rats fed with high-fat diet. Three groups of weaned male rats (ad lib. fed; rats fed with diet containing 15% w/w of citrus pectin; restrictedly pair-fed rats) were fed with either a standard diet (9.5% fat) or a high-fat diet (30% fat) for 10 days. Results: Our results revealed that pectin feeding led to significant decreases in body weight, energy intake and fat pad weight in rats fed with the standard as well as high-fat diet. Moreover, compared to the restrictedly pair-fed rats, in both groups of rats fed with the diet containing pectin, significant decrease in duodenal alkaline phosphatase (AP) activity was observed in histochemically stained cryostat sections. In contrast, despite their lower energy intake, restrictedly pair-fed rats showed similar fat pad deposition accompanied by unchanged values of AP activity in comparison to the controls. Conclusions: Our findings indicate that daily pectin consumption could be beneficial in suppressing body weight gain and reducing probability of obesity risk in rats fed with a high-fat diet.

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“…Dietary fibres possess unique chemical and physical characteristics responsible for eliciting an array of physiological responses. Soluble dietary fibres have favourable effects on gastrointestinal functions, since they can delay gastric emptying (Sanaka et al 2007), slow transit time through the small intestine (Lorenzani, 1998) and stimulate proliferation of intestinal cells (Chun et al 1989;Fukunaga et al 2003).…”

mentioning

confidence: 99%

Dietary pectin up‐regulates monocaboxylate transporter 1 in the rat gastrointestinal tract

Kirat

Kawasaki

Shimada

et al. 2009

Experimental Physiology

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This work was undertaken to study the effect of pectin feeding on the expression level, cellular localization and functional activity of monocarboxylate transporter 1 (MCT1) in the gastrointestinal tract of rats. The results indicated that MCT1 protein level was significantly increased along the entire length of the gastrointestinal tract of pectin-fed rats in comparison with control animals. Immunohistochemical analysis revealed an increase in MCT1 in the stratified squamous epithelia of the forestomach as well as in the basolateral membranes of the cells lining the gastric pit of the glandular stomach of pectin-fed rats when compared with control animals. The parietal cells, which showed barely any or no detectable MCT1 in the control group, exhibited a strong intensity of MCT1 on the basolateral membranes in pectin-fed rats. In the small intestine of pectin-fed rats, strong immunopositivity for MCT1 was detected in the brush border and basolateral membranes of the absorptive enterocytes lining the entire villi, while in control rats, weak reactivity was detected on the brush border membrane in a few absorptive enterocytes in the villus tip. In the large intestine of control animals, MCT1 was detected on the basolateral membranes of the epithelia lining the caecum and colon. This staining intensity was markedly increased in pectin-fed rats, along with the appearance of strong reactivity for MCT1 on the apical membranes of the surface and crypt epithelia of caecum and colon. Our results also showed that MCT1 co-localizes with its chaperone, basigin (CD147), in the rat gastrointestinal tract, and that the pectin feeding increased the expression of CD147. In vivo functional studies revealed an enhanced acetate absorption in the colon of pectin-fed in comparison with control animals. We conclude that MCT1 is up-regulated along the gastrointestinal tract of pectin-fed rats, which might represent an adaptive response to the increased availability of its substrates.

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Effect of Pectin, a Soluble Dietary Fiber, on Functional and Morphological Parameters of the Small Intestine in Rats

Cited by 43 publications

References 27 publications

Neutral detergent-soluble fiber improves gut barrier function in twenty-five-day-old weaned rabbits1

Neutral detergent-soluble fiber improves gut barrier function in twenty-five-day-old weaned rabbits1

Effect of pectin feeding on obesity development and duodenal alkaline phosphatase activity in Sprague-Dawley rats fed with high-fat/high-energy diet

Dietary pectin up‐regulates monocaboxylate transporter 1 in the rat gastrointestinal tract

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