Effect of Pentagastrin on Adult Rat Duodenal Cells in Culture

Intestinal tissue and cell cultures

“…Using the intestinal cell lines IEC and RIE,, several authors [24, 37, 1441 examined the effect of hormonal and growth factors on epithelial cell physiology. In primary cultures of duodenal cells (although they have not been definitely characterized), pentagastrin, which exerts a trophic effect on gastrointestinal tissue in vivo, has been shown to stimulate the proliferative activity of the cells, thus indicating a direct action of this hormone on the target cells [117]. Blay and Brown further showed that EGF regulates cell proliferation via specific, saturable receptors [23, 241.…”

Influence of proglumide on the circadian rhythm in DNA synthesis in the intestinal tract of the mouse+

“…Gastrin appears to be a trophic hormone for the oxyntic gland mucosa and the mucosa of the entire small and large intestines (Johnson and Guthrie, 1974). Several studies performed in vitro have clearly demonstrated that the trophic action of gastrin is due to a direct interaction of the hormone with receptors on its target cells and not to a secondary factor released or synthesized by gastrin (Lichtenberger et al, 1973;Miller et al, 1973;Sutton and Donaldson, 1975). Proglumide inhibits gastrin-stimulated gastric acid secretion, apparently a specific effect, because proglumide does not inhibit the secretory effects of histamine or acetylcholine (Rovati, 1968;Bali et al, 1979).…”

“…Apparently, gastrin directly stimulates DNA synthesis in the gut (Lichtenberger et al, 1973;Miller et al, 1973;Sutton and Donaldson, 1975).…”

Isolating and maintaining highly polarized primary epithelial cells from normal human duodenum for growth as spheroid-like vesicles

“…In agreement with other reports (Tsuchiya and Okada, 1982;Moyer, 1983;Nrgrel et al, 1983: QuaronL 1985, the use of nondissociated, mechanically removed mucosal fragments as starting material is essential for the successful establishment of HDEC in culture. The treatment of intestinal tissue with disintegrating proteases such as collagenase or trypsin leads to reduced brush border enzyme activity (Kedinger et al, 1986) and is also known to cause shape and polarity changes (Lichtenberger et al, 1973;Raul et al, 1978).…”

“…Many studies have been performed with cells of murine origin (Harrison and Webster. 1969;Lichtenberger et al, 1973: Raul et al, 1978: Tsuchiya and Okada. 1982N6grel et al, 1983;Kondo et al, 1984;Quaroni, 1985;Kedinger et al, 1986;Tait et al, 1992) but only a few with ceils of human ~To whom correspondence should be addressed.…”