Effect of Peptidoglycan of Staphylococcus Aureus on Apoptosis of Bovine Mammary Gland Lymphocytes

Sláma, Petr; Zavadilova, Terezie; Kratochvilova, Lucie; Kharkevich, Kristina; Uhrinčať, M.; Tančin, V.

doi:10.15414/jmbfs.2019.9.special.445-446

Cited by 2 publications

(4 citation statements)

References 9 publications

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“…Stimulation of the mammary gland by LPS or MDP caused statistically significant enhancement of the proportion of apoptotic lymphocytes 48 h following the induction of the inflammatory response. A similar trend was reported in a previous study on the effect of peptidoglycan on lymphocyte apoptosis [ 16 ]. However, a gradual increase in lymphocyte apoptosis was noted while using S. aureus or Str.…”

Section: Discussionsupporting

confidence: 90%

“…A gradual increase of apoptosis in lymphocytes during inflammation of the bovine mammary gland has been reported [ 15 ]. In another study, we investigated the effect of peptidoglycan of S. aureus on apoptosis of lymphocytes and the apoptosis of lymphocytes was found to be induced in the initial stage of mastitis [ 16 ]. The distribution of different subpopulations of lymphocytes in mammary gland secretions also changes during lactation or inflammation [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland Lymphocytes

Sláma

Kabourková

Sládek

et al. 2020

Animals

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The aim of this study was to evaluate whether apoptosis of lymphocytes is modulated by stimulation by lipopolysaccharide (LPS) of Escherichia coli or muramyl dipeptide (MDP). Cell populations were obtained by lavaging of the mammary glands 24, 48, 72, and 168 h following intramammary induced inflammation. The portion of apoptotic lymphocytes peaked at 48 h after treatment with LPS or MDP. The analysis of CD44 expression of the same cell populations showed a higher percentage of CD44-positive lymphocytes 24- and 48-h following induction of inflammation by LPS or MDP. The results demonstrate that during both experimental infection of bovine mammary glands with LPS or MDP, apoptosis of lymphocytes was induced in the initial phase of the inflammatory response and CD44 was also overexpressed at the beginning of inflammation. These data suggest a connection of lymphocyte apoptosis with the expression of CD44 receptors.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland Lymphocytes

Sláma

Kabourková

Sládek

et al. 2020

Animals

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“…In our previous study, we investigated the apoptosis of lymphocytes, which can be delayed during experimentally induced mastitis by S. uberis [ 9 ] or in in vitro through the use of the lipopolysaccharide of Escherichia coli [ 10 ]. Contrary to that, the peptidoglycan of Staphylococcus aureus is able to induce lymphocyte apoptosis during the initial stages of mastitis [ 11 ]. Similar results have been obtained in experiments involving the lipopolysaccharide of E. coli or muramyl dipeptide [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…In our previous studies [ 9 , 11 , 12 ], we have only investigated whole lymphocyte populations. Each s lymphocyte subpopulation may have a different sensitivity level for the induction of apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Effect of Streptococcus uberis on Gamma Delta T Cell Phenotype in Bovine Mammary Gland

Sláma

Zavadilova

Pavlík

et al. 2021

Animals

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In this study, we focused analyzing γδ T cells during bovine mammary gland inflammation induced by Streptococcus uberis. A mammary gland cell suspension was obtained using lavage 24, 48, 72, and 168 h after intramammary-induced infection. The proportion of lymphocytes increased during the entire week in which inflammation was present. The γδ T cells were also elevated during inflammation, reaching their peak at 72 h following induced inflammation. The percentage of apoptotic lymphocytes continually increased, with the highest proportion occurring 168 h after S. uberis infection. The results show that γδ T cells may be involved in the resolution of inflammation in bovine mammary glands, with the apoptosis of those cells potentially playing an important role.

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Effect of Peptidoglycan of Staphylococcus Aureus on Apoptosis of Bovine Mammary Gland Lymphocytes

Cited by 2 publications

References 9 publications

Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland Lymphocytes

Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland Lymphocytes

Effect of Streptococcus uberis on Gamma Delta T Cell Phenotype in Bovine Mammary Gland

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