Effect of Pesticides on the Reproductive Output of Eisenia fetida

Yasmin, Shahla; D’Souza, Doris H.

doi:10.1007/s00128-007-9269-5

Cited by 95 publications

(50 citation statements)

References 16 publications

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“…Culturing of E. fetida E. fetida were obtained from the University of Agricultural Science, Gandhi Krishi Vignana Kendra, Bangalore. The age synchronized worms were maintained according to the procedure of Yasmin and D'Souza, (2007) with slight modification. The mixture contained 75% soil and 25% cow dung for the culture of worms.…”

Section: Methodsmentioning

confidence: 99%

Effects of acute gamma radiation on the reproductive ability of the earthworm Eisenia fetida

Sowmithra

Shetty

Harini

et al. 2015

Journal of Environmental Radioactivity

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a b s t r a c tEarthworms are the most suitable biological indicators of radioactive pollution because they are the parts of nutritional webs, and are present in relatively high numbers. Four months old Eisenia fetida were exposed to different doses of gamma radiation, namely 1, 2, 3, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 & 60 Gy to study the effects of radiation on different reproductive parameters. The number of cocoons laid and the hatchlings emerged were recorded for all the selected doses. There was no reduction in cocoon production, however; decreasing size and weight of the cocoons was observed from the samples exposed to 20 Gy and above doses. Significant reductions in the hatchlings were recorded in earthworms exposed to 10 Gy and above doses. The dose response curves for a percentage reduction in hatchlings were constructed. Exposure to radiation dose of 1 and 2 Gy did not show any reduction, however, there was z10%, z50% and z90% decrease in the hatchlings in samples exposed to 3, 15 and 45, 50, 55 and 60 Gy doses respectively. Delayed hatchability was also reported at al exposure level. Histology of irradiated earthworms revealed that the structural damage in the seminal vesicles was prominent at the exposed dose of 3 Gy onwards with complete degeneration on exposure to 60 Gy of gamma radiation.

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Section: Methodsmentioning

confidence: 99%

Effects of acute gamma radiation on the reproductive ability of the earthworm Eisenia fetida

Sowmithra

Shetty

Harini

et al. 2015

Journal of Environmental Radioactivity

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“…Therefore, no cosolvent was required, and both stock solutions of glyphosate and AMPA test items were prepared in deionized water (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) …”

Section: Test Substancesmentioning

confidence: 99%

Glyphosate and aminomethylphosphonic acid chronic risk assessment for soil biota

Mérey

Manson

Mehrsheikh

et al. 2016

Enviro Toxic and Chemistry

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Glyphosate is a broad-spectrum herbicide used widely in agriculture, horticulture, private gardens, and public infrastructure, where it is applied to areas such as roadsides, railway tracks, and parks to control the growth of weeds. The exposure risk from glyphosate and the primary soil metabolite aminomethylphosphonic acid (AMPA) on representative species of earthworms, springtails, and predatory soil mites and the effects on nitrogen-transformation processes by soil microorganisms were assessed under laboratory conditions based on internationally recognized guidelines. For earthworms, the reproductive no-observed-effect concentration (NOEC) was 472.8 mg glyphosate acid equivalent (a.e.)/kg dry soil, which was the highest concentration tested, and 198.1 mg/kg dry soil for AMPA. For predatory mites, the reproductive NOEC was 472.8 mg a.e./kg dry soil for glyphosate and 320 mg/kg dry soil for AMPA, the highest concentrations tested. For springtails, the reproductive NOEC was 472.8 mg a.e./kg dry soil for glyphosate and 315 mg/kg dry soil for AMPA, the highest concentrations tested. Soil nitrogen-transformation processes were unaffected by glyphosate and AMPA at 33.1 mg a.e./kg soil and 160 mg/kg soil, respectively. Comparison of these endpoints with worst-case soil concentrations expected for glyphosate (6.62 mg a.e./kg dry soil) and AMPA (6.18 mg/kg dry soil) for annual applications at the highest annual rate of 4.32 kg a.e./ha indicate very low likelihood of adverse effects on soil biota.

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“…fetida were obtained from the University of Agricultural Science, Gandhi Krishi Vignana Kendra, Bengaluru. The age synchronized worms were maintained according to the procedure of Yasmin and D'souza, 2007 [27] with slight modification. The mixture contained 75% soil and 25% cow dung for the culture of worms.…”

Section: Culturing Of E Fetidamentioning

confidence: 99%

Evaluation of genotoxicity of the acute gamma radiation on earthworm Eisenia fetida using single cell gel electrophoresis technique (Comet assay)

Sowmithra

Shetty

Jha

et al. 2015

Mutation Research/Genetic Toxicology and Environmental Mutagene

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a b s t r a c tEarthworms (Eisenia fetida) most suitable biological indicators of radioactive pollution. Radiation-induced lesions in DNA can be considered to be molecular markers for early effects of ionizing radiation. Gamma radiation produces a wide spectrum of DNA. Some of these lesions, i.e., DNA strand breaks and alkali labile sites can be detected by the single-cell gel electrophoresis (SCGE) or comet assay by measuring the migration of DNA from immobilized nuclear DNA. E. fetida were exposed to different doses of gamma radiation, i.e., 1, 5, 10, 20, 30, 40 and 50 Gy, and comet assay was performed for all the doses along with control at 1, 3 and 5 h post irradiation to evaluate the genotoxicity of gamma radiation in this organism. The DNA damage was measured as percentage of comet tail DNA. A significant increase in DNA damage was observed in samples exposed to 5 Gy and above, and the increase in DNA damage was dose dependent i.e., DNA damage was increased with increased doses of radiation. The highest DNA damage was noticed at 1 h post irradiation and gradually decreased with time, i.e., at 3 and 5 h post irradiation. The present study reveals that gamma radiation induces DNA damage in E. fetida and the comet assay is a sensitive and rapid method for its detection to detect genotoxicity of gamma radiation.

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Effect of Pesticides on the Reproductive Output of Eisenia fetida

Cited by 95 publications

References 16 publications

Effects of acute gamma radiation on the reproductive ability of the earthworm Eisenia fetida

Effects of acute gamma radiation on the reproductive ability of the earthworm Eisenia fetida

Glyphosate and aminomethylphosphonic acid chronic risk assessment for soil biota

Evaluation of genotoxicity of the acute gamma radiation on earthworm Eisenia fetida using single cell gel electrophoresis technique (Comet assay)

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