Effect of pH adjustment, homogenization and diafiltration on physicochemical, reconstitution, functional and rheological properties of medium protein milk protein concentrates (MPC70)

Abstract

Poor solubility is the major limiting factor in commercial applications of milk protein concentrates (MPC) powders. Retentate treatments such as pH adjustment using disodium phosphate (NaHPO), also responsible for calcium chelation with homogenization and; its diafiltration with 150 mM NaCl solution were hypothesized to improve the functional properties of treated MPC70 powders. These treatments significantly improved the solubility, heat stability, water binding, dispersibility, bulk density, flowability, buffer index, foaming and emulsifying capacity of treated powders over control. Rheological behaviour of reconstituted MPC solutions was best explained by Herschel Bulkley model. Compared to rough, large globular structures with dents in control; majorly intact, separate, smaller particles of smooth surface, without any aggregation were observed in SEM micrograph of treated powders. Applied treatments are easy, cost-effective and capable to improve functional properties of treated powders that could replace control MPC70 powder in various food applications where protein functionality is of prime importance.

Effect of change in pH, heat treatment and diafiltration on properties of medium protein buffalo milk protein concentrate

“…Several changes have been reported to take place in casein micelles during UF process which include alteration in delicate salt equilibrium (Meena et al 2017), dissolution of colloidal calcium phosphate (CCP) nanoclutsers (Alexander et al 2011), swelling of casein micelles due to movement of calcium from micelles and migration of caseins, particularly j-casein from the micelle to the serum (McKenna 2000). Thereafter, aggregation of dissociated proteins during evaporation leads to migration of these protein to the air-droplet interface and their denaturation during spray drying (Bhaskar et al 2003;Meena et al 2018). Colloidal calcium also gets concentrated in retentate along with milk proteins during UF concentration of skim milk.…”

Discover of No-Side Effect Phenytoin Drug from Catharanthus Roseus Plant

“…Toxicity rate against MCF-7 cell lines will increase with the increase within the concentration of CR-NPs, as shown in Figure 7 MCF-7 activity than others reported earlier. [24][25][26][27] The CR-NPs with different concentrations was performed on MCF-7 cell and presented in Table 2 and…”

Production of Liquid Milk Protein Concentrate with Antioxidant Capacity, Angiotensin Converting Enzyme Inhibitory Activity, Antibacterial Activity, and Hypoallergenic Property by Membrane Filtration and Enzymatic Modification of Proteins

“…Furthermore, ceramic tubular membrane was used to prepare milk protein concentrate by several investigators [132][133][134][135][136]. Single stage membrane filtration [19,127,129,131,136] and ultrafiltration with diafiltration were used by several investigators to prepare milk protein concentrate [137,138]. Both continuous and discontinuous diafiltration were adopted to reduce major whey proteins, such as α-lactalbumin and β-lactoglobulin from casein fraction by ultrafiltration or nanofiltration membrane.…”