Effect of pH on the solubilities of divalent and trivalent amino acids in water at 298.15K

Lee, Ching-Yi; Chen, Jui-Tang; Chang, Wen-Thong; Shiah, I-Min

doi:10.1016/j.fluid.2013.01.010

Cited by 42 publications

(22 citation statements)

References 13 publications

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“…of HCl had been introduced to water, it is not clear, what is meant by "solute", i.e., which formula weight should be used to convert to molality. The result closest to that of Lee et al [86] suggests that "solute" refers to the zwitterion XH 2 species, not salt form, NaXH.H 2 O, that was actually added to the solution. Such ambiguities happen quite often in the nearly 850 citations in the solubility database [4].…”

Section: L-glutamic Acid Polymorphsmentioning

confidence: 62%

“…In Figure 15 the data from Sakata [85], processed using pDISOL-X, are presented in a comparison with similarly treated data from Lee et al [86]. Figure 15a shows the expected parabolic-shaped logS-pH profile of L-glutamic acid, without indication of the polymorphic form.…”

Section: L-glutamic Acid Polymorphsmentioning

confidence: 99%

“…Figure 15b shows the result of a single-point analysis of L-glutamic acid, known to be in the β-form [85], where the refined S 0 = 7.7 mg/mL. The comparable S 0 values suggest that Lee et al [86] had performed measurements on the stable β-form. It is worth pointing out that on examination of the Sakata publication [85], one encounters several shortcomings in method description, which are, unfortunately, rather common when examining published results.…”

Section: L-glutamic Acid Polymorphsmentioning

confidence: 99%

“…Figure 15. The data from Sakata [85] processed using pDISOL-X in a comparison with similarly treated data from [86].…”

Section: L-glutamic Acid Polymorphsmentioning

confidence: 99%

See 3 more Smart Citations

Equilibrium solubility measurement of ionizable drugs – consensus recommendations for improving data quality

et al. 2016

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Section: L-glutamic Acid Polymorphsmentioning

confidence: 62%

Section: L-glutamic Acid Polymorphsmentioning

confidence: 99%

Section: L-glutamic Acid Polymorphsmentioning

confidence: 99%

“…Figure 15. The data from Sakata [85] processed using pDISOL-X in a comparison with similarly treated data from [86].…”

Section: L-glutamic Acid Polymorphsmentioning

confidence: 99%

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Equilibrium solubility measurement of ionizable drugs – consensus recommendations for improving data quality

et al. 2016

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“…Despite sharing common characteristics, the aqueous solubility of amino acids range from for l -tyrosine to for l -proline (Haynes 2013 ). Although the solubility of amino acids increases under basic and acidic conditions (Lee et al 2013 ; Tseng et al 2009 ), the exploitation of this phenomenon is limited in the development of CCM, because culturing cells requires a pH near neutrality. At this pH, most amino acids are in their least soluble zwitterionic form.…”

Section: Chemical Properties Of Amino Acidsmentioning

confidence: 99%

Amino acids in the cultivation of mammalian cells

2016

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Amino acids are crucial for the cultivation of mammalian cells. This importance of amino acids was realized soon after the development of the first cell lines, and a solution of a mixture of amino acids has been supplied to cultured cells ever since. The importance of amino acids is further pronounced in chemically defined mammalian cell culture media, making the consideration of their biological and chemical properties necessary. Amino acids concentrations have been traditionally adjusted to their cellular consumption rates. However, since changes in the metabolic equilibrium of amino acids can be caused by changes in extracellular concentrations, metabolomics in conjunction with flux balance analysis is being used in the development of culture media. The study of amino acid transporters is also gaining importance since they control the intracellular concentrations of these molecules and are influenced by conditions in cell culture media. A better understanding of the solubility, stability, dissolution kinetics, and interactions of these molecules is needed for an exploitation of these properties in the development of dry powdered chemically defined media for mammalian cells. Due to the complexity of these mixtures however, this has proven to be challenging. Studying amino acids in mammalian cell culture media will help provide a better understanding of how mammalian cells in culture interact with their environment. It would also provide insight into the chemical behavior of these molecules in solutions of complex mixtures, which is important in the understanding of the contribution of individual amino acids to protein structure.Electronic supplementary materialThe online version of this article (doi:10.1007/s00726-016-2181-8) contains supplementary material, which is available to authorized users.

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Characterization of tyrosine ammonia lyases from Flavobacterium johnsoniae and Herpetosiphon aurantiacus

Virklund

Jendresen²,

Nielsen

et al. 2023

Biotechnology Journal

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Abstractp‐Coumaric acid (pCA) can be produced via bioprocessing and is a promising chemical precursor to making organic thin film transistors. However, the required tyrosine ammonia lyase (TAL) enzyme generally has a low specific activity and suffers from competitive product inhibition. Here we characterized the purified TAL variants from Flavobacterium johnsoniae and Herpetosiphon aurantiacus in terms of their susceptibility to product inhibition and their activity and stability across pH and temperature via initial rate experiments. FjTAL was found to be more active than previously described and to have a relatively weak affinity for pCA, but modeling revealed that product inhibition would still be problematic at industrially relevant product concentrations, due to the low solubility of the substrate tyrosine. The activity of both variants increased with temperature when tested up to 45°C, but HaTAL1 was more stable at elevated temperature. FjTAL is a promising biocatalyst for pCA production, but enzyme or bioprocess engineering are required to stabilize FjTAL and reduce product inhibition.

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Effect of pH on the solubilities of divalent and trivalent amino acids in water at 298.15K

Cited by 42 publications

References 13 publications

Equilibrium solubility measurement of ionizable drugs – consensus recommendations for improving data quality

Equilibrium solubility measurement of ionizable drugs – consensus recommendations for improving data quality

Amino acids in the cultivation of mammalian cells

Characterization of tyrosine ammonia lyases from Flavobacterium johnsoniae and Herpetosiphon aurantiacus

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