Effect of phophatidylcholine and phosphatidylethanolamine enrichment on the structure and function of yeast membrane

Lipids in the Structure and Function of Yeast Membrane

“…Based on the available experimental data, it could be concluded that the lipid composition of different subcellular fractions of yeast has a close resemblance to higher eukaryotic systems (Getz et al, 1968;Jakovcic et al, 1971 ;Trivedi et al, 1982b). The most predominant glycerophospholipid is PC, with exceptions in certain fractions of different strains of yeast (Anderson et al, 1964;Rank et al, 1978;Trivedi et al, 1982a). Most of the intracellular CL was found to be associated with the mitochondrial membranes.…”

“…The lipid, and especially glycerophospholipid composition of various membranous organelles, has been analyzed by several workers (Anderson et al, 1964;Getz et al, 1970Getz et al, , 1981Jakovcic et al, 1971;Cobon et al, 1974Cobon et al, , 1977Trivedi et al, 1982a). Membranes are the main structural features of most of the subcellular organelles; therefore, the relative amounts of various lipids reflect the overall cellular composition (Weete, 1980).…”

“…If grown at a temperature lower than its growth temperature, the yeast cell contains more of unsaturated fatty acids. More specific alterations in the lipids of yeast have been achieved by supplementing the growth medium of S. cerevisiae with choline or ethanolamine (Hossack et al, 1977;Trivedi et al, 1982a). Watson (1978), by growing different psychrophilic, mesophilic, and thermotolerant strains, demonstrated the effect of degree of fatty acyl unsaturation on glucose transport.…”

“…This change in fatty acyl unsaturation is accompanied by a change in the proportions of other lipids as well (Hunter and Rose, 1972). The specific enrichment of PC or PE resulted in the selective alteration of amino acid transport and osmotic fragility of S. cerevisiae cells (Hossack et al, 1977;Trivedi et al, 1982a). More specific alterations in the lipids of yeast have been achieved by supplementing the growth medium of S. cerevisiae with choline or ethanolamine (Hossack et al, 1977;Trivedi et al, 1982a).…”

“…Inositol-starved cells, where proportions of PI can be reduced to as low as 8% of total cellular phospholipids (compared with 28% in inositol-supplemented cells), undergo drastic changes in lipids, proteins, carbohydrates, and nucleic acid metabolism. Using a chol mutant of Atkinson et al (1980b), Prasad and co-workers (Trivedi et al, 1982a;Trivedi, 1982) suggested that a critical level of each phospholipid is essential for the proper functioning of yeast membrane (Section VII,A). For higher animals, various roles of PI (for example, in Ca 2+ transport and receptor functions, etc.)…”