Effect of Phosphorus Accumulation on Acetate Metabolism in the Biological Phosphorus Removal Process

Mino, Takashi; Arun, Viswanath; Tsuzuki, Yoshiaki; Matsuo, Tetsuji

doi:10.1016/b978-0-08-035592-4.50009-2

Cited by 198 publications

(169 citation statements)

References 9 publications

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“…This reserve polymer is metabolized in the subsequent aerobic phase, where readily metabolizable exogenous carbon and energy sources are no longer available, to supply the energy needed for PAO to assimilate phosphate from the mixed liquor and store it as intracellular polyphosphate granules. It now appears that the energy needed for the anaerobic uptake of volatile fatty acids and PHA synthesis is derived from hydrolysis of the intracellular polyphosphate stores, while reducing power and additional energy is generated from glycolysis of intracellular glycogen stores (Mino et al, 1987), anaerobic operation of the tricarboxylic acid (TCA) cycle (Comeau et al, 1986;Louie et al, 2000) or both (Pereira et al, 1996;Wexler et al, 2009;Zhou et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

A metabolic model for members of the genus Tetrasphaera involved in enhanced biological phosphorus removal

Kristiansen

Nguyen²,

Saunders³

et al. 2012

The ISME Journal

206

154

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Members of the genus Tetrasphaera are considered to be putative polyphosphate accumulating organisms (PAOs) in enhanced biological phosphorus removal (EBPR) from wastewater. Although abundant in Danish full-scale wastewater EBPR plants, how similar their ecophysiology is to 'Candidatus Accumulibacter phosphatis' is unclear, although they may occupy different ecological niches in EBPR communities. The genomes of four Tetrasphaera isolates (T. australiensis, T. japonica, T. elongata and T. jenkinsii) were sequenced and annotated, and the data used to construct metabolic models. These models incorporate central aspects of carbon and phosphorus metabolism critical to understanding their behavior under the alternating anaerobic/aerobic conditions encountered in EBPR systems. Key features of these metabolic pathways were investigated in pure cultures, although poor growth limited their analyses to T. japonica and T. elongata. Based on the models, we propose that under anaerobic conditions the Tetrasphaerarelated PAOs take up glucose and ferment this to succinate and other components. They also synthesize glycogen as a storage polymer, using energy generated from the degradation of stored polyphosphate and substrate fermentation. During the aerobic phase, the stored glycogen is catabolized to provide energy for growth and to replenish the intracellular polyphosphate reserves needed for subsequent anaerobic metabolism. They are also able to denitrify. This physiology is markedly different to that displayed by 'Candidatus Accumulibacter phosphatis', and reveals Tetrasphaera populations to be unusual and physiologically versatile PAOs carrying out denitrification, fermentation and polyphosphate accumulation.

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Section: Introductionmentioning

confidence: 99%

A metabolic model for members of the genus Tetrasphaera involved in enhanced biological phosphorus removal

Kristiansen

Nguyen²,

Saunders³

et al. 2012

The ISME Journal

206

154

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“…However, recognizing the apparent propensity for wild microbial consortia to synthesize the polymer (14)(15)(16), commercial production of PHA would theoretically appear to be a natural extension of wastewater treatment. In fact PHA synthesis is empirically associated with certain municipal wastewater treatment processes (17)(18)(19), although biological synthesis of PHA in full-scale wastewater treatment facilities, estimated at upwards of 4% (w/w) (data not shown), falls short of quantities necessary for commercial exploitation. Nevertheless, recognizing that many waste streams are rich in PHA precursors, the potential exists for PHA-production concurrent with wastewater treatment.…”

Section: Introductionmentioning

confidence: 99%

Functional stability of a mixed microbial consortium producing PHA from waste carbon sources

Coats

Loge

Smith

et al. 2007

Appl Biochem Biotechnol

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Polyhydroxyalkanoates (PHAs) represent an environmentally-effective alternative to synthetic thermoplastics; however, current production practices are not sustainable. In this study, PHA production was accomplished in sequencing batch bioreactors utilizing real wastewaters and mixed microbial consortia from municipal activated sludge as inoculum. Polymer production reached 85%, 53%, and 10% of the cell dry weight from methanol-enriched pulp-and-paper mill foul condensate, fermented municipal primary solids, and biodiesel wastewater, respectively. Employing denaturing gradient gel electrophoresis of 16S-rDNA from PCR-amplified DNA extracts, distinctly different communities were observed between and within wastewaters following enrichment.Most importantly, functional stability was maintained despite differing and contrasting microbial populations.

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“…These findings suggest that the genus Flavobacterium may have important environmental roles. Activated-sludge processes with cyclic changes of anaerobic and aerobic conditions have been used to remove phosphate from wastewater and are increasingly used to reduce the eutrophication process in lakes (Mino et al, 1987;Jeon & Park, 2000). An insight into the bacterial community responsible for phosphorus removal is a prerequisite for understanding and controlling the enhanced biological phosphorus removal mechanism and its processes.…”

mentioning

confidence: 99%

Flavobacterium croceum sp. nov., isolated from activated sludge

Park

Ryu

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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A Gram-negative, non-motile, rod-shaped bacterium, designated strain EMB47 T , was isolated from activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor. Growth was observed between 10 and 40 6C (optimum, 25-35 6C) and between pH 5?0 and 8?5 (optimum, pH 7?5-8?0). The predominant fatty acids of strain EMB47 T were iso-C 16 : 0 3-OH, iso-C 15 : 1 G, C 15 : 0 , iso-C 15 : 0 , iso-C 14 : 0 and iso-C 16 : 0 and it contained phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylcholine as polar lipids. The G+C content of the genomic DNA was 40?8 mol% and the major quinone was MK-6. Comparative 16S rRNA gene sequence analyses showed that strain EMB47 T formed a distinct phyletic line within the genus Flavobacterium. The levels of 16S rRNA gene sequence similarity with respect to Flavobacterium species were below 94?7 %. On the basis of the phenotypic, chemotaxonomic and molecular data, strain EMB47 T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium croceum sp. nov. is proposed. The type strain is EMB47 T (=KCTC 12611 T =DSM 17960 T ).Since the genus Flavobacterium, belonging to the phylum Bacteroidetes (formerly the Cytophaga-FlavobacteriumBacteroides group), was emended (Bernardet et al., 1996), several novel Flavobacterium species have been described: these have been isolated from diverse habitats such as micromats, fresh water, seawater, Antarctic lakes, soil, the gut of an earthworm and from sediments (McCammon & Bowman, 2000;Van Trappen et al., 2002, 2004Zhu et al., 2003; Horn et al., 2005). The physiological characteristics of members of the genus Flavobacterium are also diverse: they can be psychrophilic, psychrotolerant or mesophilic; they can be halotolerant, halophilic or sensitive to salts; and they produce a variety of enzymes (Humphry et al., 2001;Tamaki et al., 2003;Aslam et al., 2005). These findings suggest that the genus Flavobacterium may have important environmental roles. Activated-sludge processes with cyclic changes of anaerobic and aerobic conditions have been used to remove phosphate from wastewater and are increasingly used to reduce the eutrophication process in lakes (Mino et al., 1987;Jeon & Park, 2000). An insight into the bacterial community responsible for phosphorus removal is a prerequisite for understanding and controlling the enhanced biological phosphorus removal mechanism and its processes. Therefore, efforts have been made in our laboratory to isolate and characterize members of the bacterial community found in activated sludge that perform enhanced biological phosphorus removal. Here we describe the taxonomic characterization of a novel species belonging to the genus Flavobacterium.Strain EMB47 T was isolated from activated sludge that was performing enhanced biological phosphorus removal in a laboratory-scale sequencing batch reactor. Sodium acetate was supplied as a sole carbon source, and the operation of the sequencing batch reactor was as described elsewhere (Jeon et al., 2003)....

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Effect of Phosphorus Accumulation on Acetate Metabolism in the Biological Phosphorus Removal Process

Cited by 198 publications

References 9 publications

A metabolic model for members of the genus Tetrasphaera involved in enhanced biological phosphorus removal

A metabolic model for members of the genus Tetrasphaera involved in enhanced biological phosphorus removal

Functional stability of a mixed microbial consortium producing PHA from waste carbon sources

Flavobacterium croceum sp. nov., isolated from activated sludge

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