EFFECT OF PHOTOBIOMODULATION ON MUSCLE REPAIR AND OXIDATIVE STRESS CAUSED BY BOTHROPS JARARACUSSU VENOM

Abstract

INTRODUCTIONEnvenoming caused by snakes of the genus Bothrops is often associated with severe and complex local pathological manifestations, with dermonecrosis and myonecrosis being the most severe damage. The specific treatment for Bothrops accident is the use of the antivenom, with the function to neutralize the circulating venom, but its action does not extend the local manifestations. Previous studies in our group have shown that photobiomodulation using low intensity laser (LBI) increases the viability of C2C12 muscle cells when incubated with B. jararacussu venom (BjV) and further promotes the proliferation of these cells.AIMto analyze the effect of LBI on muscle repair and the oxidative stress caused by B. jararacussu venom on C2C12 myoblast cells.METHODOLOGYMyoblasts cells were plated at 1×104 cells/well and incubated for 24 hours for cell adhesion. BjV (12.5ug/ml) was applied and the cells were immediately irradiated with LBI (660 and 780 nm, power of 100 mW, irradiated time 10 sec, energy density of 4 J/cm2) and incubated for 1 h. Then, a wound was created by scratching cells with a sterile pipette tip and the cell migration into the wound surface was analyzed 24 h after the venom incubation. Oxidative stress (NO, H2O2, SOD, Gpx and TBARS) were analyzed 1 h after BjV incubation. The NO concentration was evaluated by the GRIESS and the H2O2 by the phenol red method. The concentration of SOS and Gpx was made by spectrometry. The analysis of TBARS by the Elisa method.RESULTSResults showed that the LBI was able to almost completely close the risk of the wound, similar to the control group. C2C12 cells were able to produce both NO and H2O2 one hour after the addition of the venom being statistically different from the control. LBI irradiation significantly reduced NO release, but did not alter the release of H2O2. No significant difference was observed of anti‐oxidant enzymes in C2C12 cells incubated to BjV. LBI Irradiation induced a significant increase of SOD in comparison to the venom group, but not modify Gpx release. BjV did not cause lipid peroxidation and LBI treatment did not modify this effect.CONCLUSIONPhotobiomodulation caused muscle cell protection and this protection seems to be related to decrease of reactive species of oxygen and nitrogen and increase of anti‐oxidants.Support or Funding InformationComissão de Aperfeiçoamento de Pessoal do Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.