Effect of photodynamic therapy based on indocyanine green on expression of apoptosis-related genes in human gingival fibroblast cells

Gharesi, Samira; Pourhajibagher, Maryam; Chiniforush, Nasim; Raoofian, Reza; Hashemi, Mehrdad; Shahabi, Sima; Bahador, Abbas

doi:10.1016/j.pdpdt.2017.04.007

Cited by 16 publications

(11 citation statements)

References 30 publications

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“…These data are supported by a previous study that revealed PTT/PDT with ICG at 1000 μg/mL concentrations of ICG inhibited this signaling pathway. 25 Pourhajibagher et al 44 in their recent investigations observed a reduction in the viability of cells when exposed to a light source alone. Nowak et al 37 found that light exposure can produce thermal injury in RPE cells, whereas exposure to light for longer periods can lead to photochemical damage of treated cells.…”

Section: Discussionmentioning

confidence: 95%

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The Effect of Indocyanine Green Antimicrobial Photothermal/Photodynamic Therapy on the Expression of BCL-2 and BAX Messenger RNA Levels in Human Gingival Fibroblast Cells

Pourhajibagher

Gharesi

Chiniforush

et al. 2020

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Background: Antimicrobial photothermal/photodynamic therapy (PTT/PDT) with indocyanine green (ICG) is an adjuvant therapeutic approach in the treatment of periodontitis. To explore whether PTT/PDT with ICG causes cell death by apoptosis in human gingival fibroblast (HGF) cells, BAX and BCL-2 genes expression as key events for apoptosis were evaluated in this study. Materials and methods: HGF cells were treated with: 1) different concentrations (500–2000 µg/mL) of ICG alone, 2) Diode laser irradiation alone with a fluency of 39.06 J/cm2; 3) PTT/PDT combined different concentrations (500–2000 µg/mL) of ICG with an 808 nm diode laser with a fluency of 39.06 J/cm2, and 4) controls (untreated cells). After that, BAX and BCL-2 messenger RNA levels were evaluated by real-time quantitative reverse transcription PCR. Results: PTT/PDT with 500 µg/mL of ICG caused significant increases in the expression of the BAX gene, with an 8.5-fold increase, which was approximately 7- and 8.5-fold higher than PTT/PDT with ICG for 1500 and 2000 µg/mL of ICG, respectively, indicating induction of apoptosis in HGF cells. ICG (in different test concentrations), diode laser, and PTT/PDT with ICG (1500 and 2000 µg/mL of ICG) treatment displayed insignificant increases in expression levels of BAX (all p>0.05). Our experiments showed an insignificant increase (1.1–1.6-fold) in the expression of BCL-2 after ICG, diode laser, and PTT/PDT with ICG treatment (all p>0.05). Conclusions: This study suggests that various concentration of ICG can be the diverse expression of BAX responses to PTT/PDT on HGF cells.

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Section: Discussionmentioning

confidence: 95%

“…For experiments requiring seeding of HGF cells in microplates, in the third passages, a density of 2×10 6 HGF cells/well were seeded in flat-bottom 96-well cell culture microplates (Greiner Bio-One, Germany). 25…”

Section: Cell Culturementioning

confidence: 99%

The Effect of Indocyanine Green Antimicrobial Photothermal/Photodynamic Therapy on the Expression of BCL-2 and BAX Messenger RNA Levels in Human Gingival Fibroblast Cells

Pourhajibagher

Gharesi

Chiniforush

et al. 2020

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“…Photosensitizers such as chlorin e6 (Ce6) and methylene blue (MB), aminolevulinic acid (ALA), silicon phthalocyanine (Pc4), m‐tetrahydroxyphenylchlorin (mTHPC), indocyanine green (ICG), and mono‐ l ‐aspartyl chlorin e6 (NPe6), has the ability to generate singlet oxygen species. Ce6 is the most commonly used PS molecule due to its rapid removal from the body and high singlet oxygen generation efficiency, with absorption peaks around 410 and 665 nm.…”

Section: Gold Nanorodsmentioning

confidence: 99%

Advanced Near‐Infrared Light‐Responsive Nanomaterials as Therapeutic Platforms for Cancer Therapy

Chien

Chan

Anderson

et al. 2018

Advanced Therapeutics

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Cancer is one of the leading causes of death. Despite the huge progress in the field of cancer drugs and therapies, the treatment outcome is still bleak for most cancer patients. Nanotechnology has revolutionized cancer therapeutics. By using nano-sized particles as delivery systems, therapeutic biomolecules are transported efficiently to the target sites. Moreover, these particles are designed to carry out multiple cancer treatments simultaneously. Near-infrared (NIR) light-responsive nanomaterials have gained much attention as NIR light has a greater penetration depth, minimal phototoxicity, lower autofluorescence, and reduced light scattering. Among the available NIR light-responsive nanomaterials, gold nanorods, upconversion nanoparticles, carbon dots, transition metal dichalcogenide, metal oxides, black phosphorus, and polymeric nanomaterials have become attractive options owing to their excellent optical properties, ease of synthesis and modification, outstanding photodynamic and photothermal conversion properties, and most importantly, favorable toxicity level and biocompatibility which are prerequisites for biological applications. In this review, the outstanding properties, synthesis, and surface functionalization of the aforementioned NIR light-responsive nanomaterials are introduced in detail. Recent advances of these nanomaterials for various cancer treatment modalities are summarized to highlight their versatility and potential in cancer theranostics. Finally, a perspective is proposed on future research directions and their clinical translation.

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“…Also, recent researches confirmed its potential as antitumor, antioxidant, antimicrobial and anti-inflammatory (AGGARWAL et al, 2003;CRIVELLO et al, 2005;DUVOIX et al, 2005). It has a maximum absorption with blue LED on wavelength of 430 nm, presenting a strong phototoxic effect in microorganisms even when used in lower concentrations (GHARESI et al, 2017;SAITAWEE et al, 2018).…”

Section: Introductionmentioning

confidence: 98%

“…Briefly, molecules of MB incorporated to cellular structures of microorganisms are able to absorb photons and excite electrons. These electrons are transferred to a substrate or molecular oxygen, resulting in superoxides that can damage or kill microbial cells (CHIAVIELLO et al, 2011;GHARESI et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the cytotoxic effect of antimicrobial photodynamic therapy on fibroblasts (NIH/3T3) and expression of Bcl-2 family genes

Lamarque¹

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Effect of photodynamic therapy based on indocyanine green on expression of apoptosis-related genes in human gingival fibroblast cells

Cited by 16 publications

References 30 publications

The Effect of Indocyanine Green Antimicrobial Photothermal/Photodynamic Therapy on the Expression of BCL-2 and BAX Messenger RNA Levels in Human Gingival Fibroblast Cells

The Effect of Indocyanine Green Antimicrobial Photothermal/Photodynamic Therapy on the Expression of BCL-2 and BAX Messenger RNA Levels in Human Gingival Fibroblast Cells

Advanced Near‐Infrared Light‐Responsive Nanomaterials as Therapeutic Platforms for Cancer Therapy

Evaluation of the cytotoxic effect of antimicrobial photodynamic therapy on fibroblasts (NIH/3T3) and expression of Bcl-2 family genes

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