EFFECT OF PHOTOPERIODICITY ON CO2 FIXATION BY Chlorella vulgaris Buitenzorg IN BUBBLE COLUMN PHOTOBIOREACTOR FOR FOOD SUPPLEMENT PRODUCTION

Wijanarko, Anondho; Dianursanti, Dianursanti; Witarto, Arief Budi; Soemantojo, Roekmijati Widaningroem

doi:10.7454/mst.v8i2.254

Cited by 9 publications

(12 citation statements)

References 18 publications

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“…This is for the reason that the incidence of adequate light energy under continuous light in the 1 st week of cultivation, during cell metabolism process, therefore, C. vulgaris is able to grow speedily. Similar observations were reported by scientists [22].After 3 rd week onwards, in both sets of continuous light, the growth and chlorophyll content were reduced and carotenoid content was increased rapidly. This is because of photooxidation reaction in the cells, owed to excess light that cannot be absorbed by the photosynthetic apparatus.…”

supporting

confidence: 78%

Effects of Culture Conditions on Growth and Biochemical Profile of Chlorella Vulgaris

Sharma¹,

Singh²,

Sharma³

2012

J Plant Pathol Microb

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The effects of culture conditions at different temperature and light regimes on growth and the contents of chlorophyll-a, chlorophyll-b, total carotenoids, total protein and total free amino acids of Chlorella vulgaris were determined. The growth of C. vulgaris in terms of optical density (0.42 at 670 nm), cell count (440 x 104 cells/ml) and dry weight (30.2 mg/50 ml), and the amount of chlorophyll-a (2.16%), chlorophyll-b (0.59%) and total protein, was found higher at the temperature 25-30°C and natural day light receiving through the north facing window of the growth room. Although, the amount of total carotenoids (0.440%) and free amino acids (834 µg/gm fresh weight) were found maximum in continuous light at 30-35°C, not much differences in the amount of carotenoids (0.385%) and free amino acids (822 µg/gm fresh weight) were found at 25-30°C and natural day light. The natural day light at 25-30°C was also proved proficient, as distinct banding pattern with unique polypeptides such as 15KDa, 47KDa and 50KDa, on the other hand, 23KDa, 26KDa and 36KDa appeared in all samples, these bands were not affected by light and temperature. Our results indicate that among all five culture conditions tested, the cultures kept at north facing window receiving natural day light at temperature 25-30°C, show best growth and higher contents of biochemicals that will be beneficial to use Chlorella for high nutritive purpose. In order to find out the optimum culture condition, the cultures were subjected to five different conditions of temperature and light regimes. In the growth room, light was provided by fluorescent tube lights (40 W) having 2500 Lux intensity, and were fixed at a distance of 64 cm from the cultures in constant and alternate light conditions. One set of five different culture conditions were placed in the laboratory in window, which was facing north, for providing natural day and dark periods. The intensity of natural day light was as an average 2700 Lux. The following culture conditions set in the growth room were used in the present study: Effects of Culture Conditions on Growth and Biochemical Profile of Chlorella Vulgaris Rekha Growth measurement Estimation of pigmentsChlorophyll content of the samples were extracted in 90% (v/v) acetone, and chlorophyll-a, b estimated by Parson and Strickland method [14]. Carotenoid content of the samples were extracted in 80% (v/v) acetone, and estimated by Jensen method [15]. Estimation of proteinsProtein content of the samples was estimated quantitatively by Lowry [16] by dry biomass, using bovine serum albumin (BSA) as standard. Qualitatively, the proteins were estimated by the SDS-PAGE analysis, which was carried out according to Laemmli [17]. For extraction of proteins, the samples were homogenized with lysis buffer containing 0.5M Tris-HCl, 8M Urea, 5% (w/v) SDS, 20% (v/v) Glycerol and 10% (v/v) β-Mercaptoethanol; final pH 6.8 and centrifuged at 4°C for 20 min at 10,000 rpm. Protein extract was used for SDS-PAGE analysis, using a 12% polyacrylamide gel co...

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supporting

confidence: 78%

Effects of Culture Conditions on Growth and Biochemical Profile of Chlorella Vulgaris

Sharma¹,

Singh²,

Sharma³

2012

J Plant Pathol Microb

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“…The decrease of microbial CO 2 fixation ability in this serial reactor with periodic illumination is hardly different to the result with alteration of illumination [7][8][9]. Compare to the result in single reactor, It was predicted that individual cellular growth ability after dark period using CO 2 more efficient [6]. …”

Section: Y Qmentioning

confidence: 88%

“…continuous illumination, cycle illumination, and alteration illumination) at a single photo bioreactor [2][3][4][5][6]. This research uses several photo bioreactors configured in series to increase the amount of biomass and CO 2 fixation by using daily cycle periodic illumination.…”

Section: Introductionmentioning

confidence: 99%

BIOMASS PRODUCTION Chlorella vulgaris BUITENZORG USING SERIES OF BUBBLE COLUMN PHOTO BIOREACTOR WITH A PERIODIC ILLUMINATION

Wijanarko¹,

Dianursanti²,

Muryanto³

et al. 2010

MST

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Chlorella vulgaris Buitenzorg cultivation using three bubble column photo bioreactors arranged in series with a volume of 200 mL for 130 hours shows an increase of biomass production of Chlorella vulgaris Buitenzorg up to 1.20 times and a decrease of the ability of CO 2 fixation compared to single reactor at a periodic sun illumination cycle. The operation conditions on cultivation are as following: T, 29.0 o C; P,1 atm.; U G , 2.40 m/h; CO 2 , 10%; Benneck medium; and illumination source by Phillip Halogen Lamp 20W /12V/ 50Hz. Other research parameters such as microbial carbon dioxide transferred rate (qco 2 ), CO 2 transferred rate (CTR), energy consumption for cellular formation (E x ), and cultural bicarbonate species concentration [HCO 3 ] also give better results on series of reactor.

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“…High cell activity allows a higher CO 2 concentration gradient between bulk and cell membranes. This concentration gradient becomes an influential driving force, as predicted by Wijanarko et al (2004).…”

Section: Resultsmentioning

confidence: 99%

Utilization of Chlorella vulgaris to Fixate a High Concentration of Carbon Dioxide in a Compost-based Medium

Ismail¹

2016

IJTech

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Massive use of fuels by industry increase carbon dioxide (CO 2 ) emissions significantly. Chlorella vulgaris (C. vulgaris) is well known for its ability to fixate CO 2 and synthesize it to a lipid. As industry usually emits high concentrations of CO 2 , it is necessary to investigate the behavior of microalgae in regard to CO 2 inflow. We studied cultivation of C. vulgaris in a photobioreactor (volume 18L) in a compost-basedmedium under illumination at 3000 lux for 90 hours. We show that initial cell density 0.137 g·dm -3 is able to fixate CO 2 up to 30.31 g·dm -3 · day -1 (93.56%) under a CO 2 inflow of 23.80 g·hour -1 with biomass productivity 0.44 g·dm -3

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EFFECT OF PHOTOPERIODICITY ON CO2 FIXATION BY Chlorella vulgaris Buitenzorg IN BUBBLE COLUMN PHOTOBIOREACTOR FOR FOOD SUPPLEMENT PRODUCTION

Cited by 9 publications

References 18 publications

Effects of Culture Conditions on Growth and Biochemical Profile of Chlorella Vulgaris

Effects of Culture Conditions on Growth and Biochemical Profile of Chlorella Vulgaris

BIOMASS PRODUCTION Chlorella vulgaris BUITENZORG USING SERIES OF BUBBLE COLUMN PHOTO BIOREACTOR WITH A PERIODIC ILLUMINATION

Utilization of Chlorella vulgaris to Fixate a High Concentration of Carbon Dioxide in a Compost-based Medium

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