Effect of plant growth regulators on in vitro plant regeneration of sandalwood (Santalum album L.) via organogenesis

Singh, Chandrakant; Raj, Sandeep R.; Jaiswal, Pooja; Patil, V. R.; Punwar, B. S.; Chavda, J. C.; Subhash, Narayanan

doi:10.1007/s10457-015-9853-3

Cited by 37 publications

(21 citation statements)

References 15 publications

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“…Nevertheless, in our study, we verified a considerable increment of adventitious shoots with BAP supplementation. The reason for the increase in the number of shoots is because CKs promote the growth of axillary shoots and end apical dominance (Singh et al, 2016). On the other hand, with high CK concentrations, plants may face a higher level of stress, which can activate genes related to synthesis of cytokinin oxidases/dehydrogenases, which in turn bind to specific sites of the substrate, causing CK catalysis (Kopečný et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP

et al. 2020

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During in vitro propagation, cytokinins (CKs) and auxins (AUXs), such as 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA), are often used to induce adventitious shoots and roots, respectively. However, it is not clear how CKs affect plants over a long period of in vitro propagation as well as the synergy of direct exposure to AUX with previous CK treatments. The aim was to assess the physiological and anatomical responses of Alcantarea imperialis in function of the interaction of both previous BAP treatments and direct NAA exposure during in vitro propagation. Plants previously grown in vitro were transferred to media containing 0, 5, 10 or 15 μM BAP. After 60 days, the adventitious shoots from each previous BAP treatment were subcultured in media with 0, 2 or 4 μM NAA. Pigment content, anatomical and growth traits were assessed in the plants from each treatment. Both previous BAP treatments and direct NAA exposure altered the anatomy and pigment contents of plants as well as their growth traits. BAP induced negative effects over the long term on physiological status as well as changed the plants’ anatomy. NAA supplementation in the medium can partially reverse the negative effects induced by BAP. The application of 2 μM NAA during in vitro rooting improved the plants’ quality.

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Section: Discussionmentioning

confidence: 99%

Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP

et al. 2020

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“…During these two stages of the micropropagation process, the levels required by exogenous phytohormones in the culture medium may vary. Therefore, the success of in vitro plant regeneration is dependent on explant response by supplementation of phytohormones in the culture medium [ 183 ].…”

Section: Physiological Responses Of Plant Regenerationmentioning

confidence: 99%

Cellular, Molecular, and Physiological Aspects of In Vitro Plant Regeneration

Bidabadi

Jain

2020

Plants

162

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Plants generally have the highest regenerative ability because they show a high degree of developmental plasticity. Although the basic principles of plant regeneration date back many years, understanding the cellular, molecular, and physiological mechanisms based on these principles is currently in progress. In addition to the significant effects of some factors such as medium components, phytohormones, explant type, and light on the regeneration ability of an explant, recent reports evidence the involvement of molecular signals in organogenesis and embryogenesis responses to explant wounding, induced plant cell death, and phytohormones interaction. However, some cellular behaviors such as the occurrence of somaclonal variations and abnormalities during the in vitro plant regeneration process may be associated with adverse effects on the efficacy of plant regeneration. A review of past studies suggests that, in some cases, regeneration in plants involves the reprogramming of distinct somatic cells, while in others, it is induced by the activation of relatively undifferentiated cells in somatic tissues. However, this review covers the most important factors involved in the process of plant regeneration and discusses the mechanisms by which plants monitor this process.

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“…Considerable work have been carried out in sandalwood by many researchers all over the world using diverse explants such as embryo [7,8], hypocotyls [2,8], shoot tip [9,10], nodal segment [2, 9,11,12,13,14,15], leaf disc [5,16,17], seedling [18], endosperm [2], cotyledons [8], protoplast [4,19,20,21] and cell suspension cultures [22,23] with varying degree of success. Somatic embryogenesis in higher frequencies have been reported by Herawan et al [24], Ilah et al [25] and Isah [26].…”

Section: Introductionmentioning

confidence: 99%

Massive in vitro Cloning of Sandalwood (Santalum album Linn.) via Cultured Nodal Segments

Bele¹,

Tiwari²,

Tripathi³

et al. 2019

CJAST

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Nodal segments of sandalwood were cultured on MS medium amended with different plant growth regulators in varying concentrations to search out higher in vitro response leading to plantlet regeneration via somatic embryogenesis and/or organogenesis. Higher proportion of direct somatic embryogenesis, number(s) of somatic embryo per explant and plantlet regeneration via direct organogenesis were recorded on MS medium supplemented with a moderate concentration of TDZ (1.0 mgl-1) in combination with comparatively a lower concentration of NAA (0.5 mgl-1). A relative higher concentration of BAP (1.0-2.0 mgl-1) in combination with a lower concentration of NAA (0.5 mgl-1) promoted frequency of indirect somatic embryogenesis. Ratio of organ formation directly from surface of cultured explants was recovered from culture medium fortified with a higher concentration of BA at the concentration of 4.0 mgl-1 in combination with a lower concentration of NAA (0.5 mgl-1). Maximum plantlets regenerated via somatic embryogenesis (direct and/or indirect) on regeneration medium supplemented with 2.0 mgl-1TDZ in combination with 1.0 mg l-1GA3, while plantlets in higher frequencies via indirect organogenesis was attained with regeneration medium amended with comparatively lower concentration of TDZ (1.0 mg l-1) in combination with 0.5 mgl-1 GA3 and 0.5 mgl-1 NAA. The plantlets were transferred to pots and hardened in Environmental Growth Cabinet and Net House during preliminary weaning period and transferred to field successfully. Morphologically normal plants were recovered.

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Effect of plant growth regulators on in vitro plant regeneration of sandalwood (Santalum album L.) via organogenesis

Cited by 37 publications

References 15 publications

Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP

Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP

Cellular, Molecular, and Physiological Aspects of In Vitro Plant Regeneration

Massive in vitro Cloning of Sandalwood (Santalum album Linn.) via Cultured Nodal Segments

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