Effect of plant growth regulators, temperature and sucrose on shoot proliferation from the stem disc of Chinese jiaotou (Allium chinense) and in vitro bulblet formation

Zhang, Xu; Um, Yeongcheol; Kim, Chun-Hwan; Lü, Gang; Guo, Danlu; Liu, Hailin; Bah, Amadou Apho; Mao, Aining

doi:10.1007/s11738-008-0150-x

Cited by 40 publications

(19 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This was shown by Su et al (2011) who found that a low concentration of auxin combined with cytokinin might aid in shoot initiation. Hence, the ratio of cytokinin to auxin is a critical determinant of organogenesis in plant tissue culture (Xu et al, 2008). Aggarwal et al (2010) found that the combination of auxin and cytokinin is effective in inducing shoot from the leaf explant of Eucalyptus tereticornis.…”

Section: Shoot Induction From In Vitro Stem Explantsmentioning

confidence: 99%

Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata

Ling

Tan

Hussein

2013

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Abstract:Objective: Labisia pumila var. alata, commonly known as 'Kacip Fatimah' or 'Selusuh Fatimah' in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medicinal properties. Its various pharmaceutical applications cause an excessive harvesting and lead to serious shortage in natural habitat. Thus, this in vitro propagation study investigated the effects of different plant growth regulators (PGRs) on in vitro leaf and stem explants of L. pumila. Methods: The capabilities of callus, shoot, and root formation were evaluated by culturing both explants on Murashige and Skoog (MS) medium supplemented with various PGRs at the concentrations of 0, 1, 3, 5, and 7 mg/L. Results: Medium supplemented with 3 mg/L indole-3-butyric acid (IBA) showed the optimal callogenesis from both leaf and stem explants with (72.34±19.55)% and (70.40±14.14)% efficacy, respectively. IBA was also found to be the most efficient PGR for root induction. A total of (50.00±7.07)% and (77.78±16.47)% of root formation were obtained from the in vitro stem and leaf explants after being cultured for (26.5±5.0) and (30.0±8.5) d in the medium supplemented with 1 and 3 mg/L of IBA, respectively. Shoot formation was only observed in stem explant, with the maximum percentage of formation ((100.00±0.00)%) that was obtained in 1 mg/L zeatin after (11.0±2.8) d of culture. Conclusions: Callus, roots, and shoots can be induced from in vitro leaf and stem explants of L. pumila through the manipulation of types and concentrations of PGRs.

show abstract

Section: Shoot Induction From In Vitro Stem Explantsmentioning

confidence: 99%

Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata

Ling

Tan

Hussein

2013

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

show abstract

“…Therefore, MS medium was the most effective for growth in I. odaesanensis. BA plays a key role in shoot regeneration in vitro (Ayabe et al 1995;Ayabe et al 1998;Guo et al 2005;Xu et al 2008). In the present experiment, BA enabled shoot regeneration at a frequency of up to 100% when rhizome explants were cultured on medium with 1.0 or 3.0 mg/l BA, although BA at a level of 3.0 mg/l showed a suppressive effect on shoot differentiation (Table 2).…”

Section: Resultsmentioning

confidence: 50%

Plant regeneration from callus of Iris odaesanensis Y. N. Lee native to Korea via organogenesis

Bae¹,

Yoo²,

Lee³

et al. 2013

Journal of Plant Biotechnology

View full text Add to dashboard Cite

Iris odaesanensis Y. N. Lee. is an important endangered and native plant belonging to the family Iridaceae in Korea. This study describes a method for rapid micropropagation of this species via from leaf, rhizome and root explants derived calli. Leaf, rhizome and root explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) for callus induction. Rhizome explants yielded calli at a frequency of 72% when cultured at 1.0 mg/l 2,4-D. Calli were maintained at 1.0 mg/l 2,4-D. These calli were transferred to MS medium supplemented with 0, 0.5, 1.0, and 2.0 mg/l 2,4-D in combination with 0, 0.5, 1.0, and 3.0 mg/l BA for adventitious shoot induction. The highest number of adventitious shoot (228.9 per petri-dish) were formed at 1.0 mg/l 2,4-D and 1.0 mg/l BA. WPM medium was the best to convert calli into plantlets, where up to 98.2% of calli were regenerated into plantlets. This in vitro propagation protocol should be useful for conservation of this endangered plant.

show abstract

“…1F). BA plays a key role in shoot regeneration in vitro (Ayabe et al 1995;Ayabe and Sumi 1998;Guo et al 2005;Xu et al 2008). In the present experiment, BA induced shoot regeneration at the rate of 100% when cultured on medium with 1.0 or 3.0 mg･L -1 BA, although the highest BA concentration at 3.0 mg･L -1 appeared to show a suppressive effect on shoot differentiation (Table 2).…”

Section: Plantlet Production and Soil Transfermentioning

confidence: 48%

Callus induction and plant regeneration of Iris dichotoma Pall. in endangered species

Bae¹,

Yoo²,

Lee³

et al. 2012

Journal of Plant Biotechnology

View full text Add to dashboard Cite

Iris dichotoma Pall. is an important endangered plant belonging to the family Iridaceae. A method was developed for the rapid micropropagation of I. dichotoma through plant regeneration from leaf, rhizome, and root explant-derived calli. Leaf, rhizome, and root segments were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D; 0-3.0 mg･L IBA, on which 98% of the regenerated shoots developed roots with an average of 3.5 roots per shoot within 45 days.The plantlets raised in vitro were acclimatized and transferred to soil with 95% success. This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.

show abstract

Effect of plant growth regulators, temperature and sucrose on shoot proliferation from the stem disc of Chinese jiaotou (Allium chinense) and in vitro bulblet formation

Cited by 40 publications

References 33 publications

Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata

Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata

Plant regeneration from callus of Iris odaesanensis Y. N. Lee native to Korea via organogenesis

Callus induction and plant regeneration of Iris dichotoma Pall. in endangered species

Contact Info

Product

Resources

About