Effect of Planting Media and Different Concentration of Wheat Grass Juice (Triticum aestivum L.) on Anemic Male Mice (Mus musculus L.) Leukocytes

Abstract

Iron deficiency can cause anemia. Changes in iron status in anemia can affect the immune system, making infection easy. When the number of infections increases, efforts made to overcome anemia are one of them by consuming wheat grass containing iron. This study was an experiment with factorial complete randomized design (CRD). The number of samples was 40 male mice aged 8-10 weeks, weighing 20-30 g which were grouped into 8 treatment groups and each consisted 5 of mice. Treatment K1: not given wheat grass juice (control), K2: not given juice wheat grass after induction of NaNO2 (anemia treatment), P1T: group given 50% wheat grass juice which was planted with soil after anemic treatment. P2T: a group that was given wheat grass juice with a concentration of 75% which was planted with soil after anemic treatment and P3T: a group that was given wheat grass juice with a concentration of 75% planted with soil after anemic treatment. P1H: groups given 50% wheat grass juice planted hydroponically after anemic treatment. P2H: a group given 75% wheat grass juice which was planted hydroponically after anemic treatment. P3H: group given 100% wheat grass juice which is planted hydroponically after anemic treatment. Taking blood was taken before and after giving wheat grass juice for 14 days through the tip of the tail of mice. Data were analyzed using ANOVA followed by DMRT follow-up with a level of 5%. The results showed that the planting medium had no effect on male leukocytes where Fcount> Ftable. Giving wheat grass juice with different concentrations has an effect on Leukocytes where Fcount> Ftable. The most obvious treatment for the wheat grass juice with 100% concentration.