Effect of plasma triglyceride concentrations on the accuracy of immunoturbidimetric assays of apolipoprotein B

Abstract

We have studied the influence of triglyceride-rich particles on the analytical bias of apolipoprotein B measurements by various immunoturbidimetric methods. Three commercially available methods grossly overestimate apolipoprotein B in samples with even moderately above-normal triglyceride concentrations. This effect is due to the increased relative reactivity of very-low-density lipoproteins in these reagent systems, and can be eliminated by including Tween 20 (2 g/L) in the reagent buffer. We have developed, and describe, an automated immunoturbidimetric method that allows the accurate determination of apolipoprotein B in the plasma of patients with hypertriglyceridemia.

A Comparison of the Effects of Raloxifene and Conjugated Equine Estrogen on Bone and Lipids in Healthy Postmenopausal Women

“…The triglyceride effect of CEE may have artifactually increased apolipoprotein B measurements by interfering with its assay. 30 Data from a subset of this study population and from another study suggest that there may also be differences in their effects on plasminogen activator inhibitor 1 and Creactive protein. 31,32 The present findings are similar to those in previous studies of estrogen and shorter-term studies of raloxifene.…”