Effect of platelet-rich plasma (PRP) concentration on the viability and proliferation of alveolar bone cells: an in vitro study

Choi, Byung Ho; Zhu, Shi Jiang; Kim, B. Y.; Huh, Jin Young; Lee, S. H.; Jung, Jae Hyung

doi:10.1016/j.ijom.2004.10.018

Cited by 216 publications

(173 citation statements)

References 20 publications

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“…In fact, Group AB/PRP-150 had a significantly greater RPA than all other groups. The cytotoxic effect of this volume of PRP may be explained by the findings of Choi et al 19 They demonstrated that cell response was more cytotoxic to PPP than to PRP. Because PPP is of the constituents of PRP and is directly related to its volume, a more intense cytotoxicity may be expected when a large volume of PRP is combined with autogenous bone graft.…”

Section: Discussionmentioning

confidence: 92%

“…One explanation for these findings is that when a small amount of graft is mixed with a large volume of PRP the bone cells in both the autograft and adjacent tissue are exposed to high PRP concentrations. In an in vitro study, Choi et al 19 showed that the viability and proliferation of alveolar bone cells are stimulated by low, but suppressed by high concentrations of PRP. In a recent in vivo study, Nagata et al 25 demonstrated that a large volume of PRP combined with autogenous bone graft decreased the expression of osteopontin.…”

Section: Discussionmentioning

confidence: 99%

“…11 The controversies regarding the influence of PRP on wound healing should be reexamined considering many variables, including the ratio of particulate autogenous bone graft/PRP. According to Choi et al, 19 a dilution of the PRP concentrations to a level suitable for cell proliferation may be necessary for use in bone grafts to enhance bone cell growth within the PRP-treated bone graft. Within the limits of this study, it can be concluded that the ratio autogenous bone graft/PRP influences bone healing in CSD in rat calvaria.…”

Section: Discussionmentioning

confidence: 99%

“…17 The literature lacks information regarding the biologic significance of different proportions of GFs within PRP preparations in humans and large animals. 18 In an in vitro study, Choi et al 19 found that the viability and proliferation of alveolar bone cells were suppressed by high PRP concentrations, but were stimulated by low concentrations. Furthermore, they suggested that variations in PRP concentration might influence bone formation within bone grafts.…”

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confidence: 99%

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Influence of the ratio of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects: A histologic and histometric study in rat calvaria

et al. 2009

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The purpose of this study was to analyze histomorphometrically the influence of the ratio of particulate autogenous bone (AB) graft/platelet-rich plasma (PRP) on bone healing in surgically created critical-size defects (CSD) in rat calvaria. Fifty rats were divided into five groups: Group C (control), Group AB, Group AB/PRP-50, Group AB/PRP-100, and Group AB/PRP-150. A 5-mm diameter critical-size defect was created in the calvarium of each animal. In Group C, the defect was filled by blood clot only. In Group AB, the defect was filled with 0.01 mL of AB graft. In Groups AB/PRP-50, AB/PRP-100, and AB/PRP-150, the defects were filled with 0.01 mL of AB graft combined with 50, 100, and 150 mL of PRP, respectively. All animals were euthanized at 30 days postoperative. Histomorphometry, using image analysis software, and histology analyses were performed. New Bone Area (NBA) and the remaining bone graft particles area (RPA) were calculated as a percentage of the total area of the original defect. Percentage data were transformed into arccosine for analysis. No defect completely regenerated with bone. Group AB/PRP-50 (41.78 AE 13.48%) had a significantly greater NBA than Groups C (19.29 AE 5.11%), AB (27.43 AE 10.90%) or AB/PRP-150 (19.17 AE 8.45%) (p < 0.05). No significant differences were observed between groups AB/PRP-50 and AB/ PRP-100 or among groups AB, AB/PRP-100, and AB/PRP-150 with regard to NBA (p > 0.05). Group AB/PRP-150 (31.59 AE 3.22%) had a significantly greater RPA than Groups AB (19.09 AE 5.21%), AB/PRP-50 (17.33 AE 4.43%), and AB/PRP-100 (19.72 AE 3.62%) (p < 0.001). No significant differences were observed among groups AB, AB/PRP-50, and AB/PRP-100 with regard to RPA (p > 0.05). The ratio AB graft/PRP influences bone healing in surgically created CSD in rat calvaria. ß

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Influence of the ratio of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects: A histologic and histometric study in rat calvaria

et al. 2009

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“…Arpormaeklong et al 13 (rat bone marrow stromal cells, rhBMP2, PPP = platelet-poor plasma) demonstrated the PRP as estimulator of the cellular proliferation (dose-dependent), and inhibitor of the activity of alkaline phosphatase and calcium deposition. Choi et al 37 (alveolar bone cells, PPP, PC = platelets concentrate without plasma) observed that PRP in high concentrations suppress the viability and the cellular proliferation, however there is a stimulation effect in low concentrations (1-5%). Also observed cytotoxic answers with the PPP, and with PC an increase in the viability and the proliferation cellular of significant form.…”

Section: Discussionmentioning

confidence: 99%

Effects of a highly concentrated platelet-rich plasma on the bone repair using non-critical defects in the calvaria of rabbits

et al. 2010

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PURPOSE: To verify the effect of highly concentrated platelet-rich plasma (hPRP) in the pathways of bone repair using non-critical defects in the calvaria of rabbits. METHODS: The hPRP was produced from collected venous blood of 21 rabbits. Four non-critical defects of 8 mm in diameter were created on the calvaria of each animal. The defects were all treated differently: autogenous particled bone (APB, group 1), autogenous particled bone associated with hPRP (APB + hPRP, group 2), isolated hPRP (group 3), and blood clot (control, group 4). Animals were submitted to euthanasia on the 2nd, 4th and 6th week postoperatively. Histological and histomorphometric analysis were carried through. RESULTS: After two weeks, groups 1 and 2 were in more advanced stage of repair than 3 and 4. At this period, comparing the groups 1 and 2, no significant differences were found between both, which also happened between the groups 3 and 4. However, after four and six weeks, the group 1 showed a more advanced stage of repair among all the other studied groups, while group 2 was in more advanced signs of bone repair than groups 3 and 4. Comparing groups 3 and 4, after four and six weeks, the least advanced stage of bone repair was found to be within group 3. CONCLUSION: The use of a highly concentrated PRP was considered prejudicial to the repair of non-critical defects in the calvaria of rabbits, either in the association of autogenous particled bone, when compared to autogenous particled bone alone, or in its isolated form, when compared to blood clot (control).

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Bone Bioengineering: Scaffolds, Growth Factors, and Stem Cells

Lee

Hermann

Gittens

et al. 2013

Stem Cells in Craniofacial Development and Regeneration

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Effect of platelet-rich plasma (PRP) concentration on the viability and proliferation of alveolar bone cells: an in vitro study

Cited by 216 publications

References 20 publications

Influence of the ratio of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects: A histologic and histometric study in rat calvaria

Influence of the ratio of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects: A histologic and histometric study in rat calvaria

Effects of a highly concentrated platelet-rich plasma on the bone repair using non-critical defects in the calvaria of rabbits

Bone Bioengineering: Scaffolds, Growth Factors, and Stem Cells

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