Effect of pollination time, the hour of daytime, pollen storage temperature and duration on pollen viability, germinability, and fruit set of date palm (Phoenix dactylifera L.) cv "Deglet Nour"

Kadri, Karim; Elsafy, Mohammed; Makhlouf, Souhayla; Awad, Mohamed A.

doi:10.1016/j.sjbs.2021.09.062

Cited by 17 publications

(12 citation statements)

References 9 publications

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“…The obtained fresh pollen grains had 92% viability and 94% germination as determined by acetocarmine staining and in vitro germination tests, respectively. Recently, In vitro tests revealed that fresh pollens had the highest viability (96%) and germination (85%) and result in the greatest fruit percentage as compared with stored pollens (Kadri et al, 2022). Thus, increasing pollen application in female inflorescences does not guarantee high FS unless the pollen used is viable and has a high degree of germination (Kav et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

“…Thus, increasing pollen application in female inflorescences does not guarantee high FS unless the pollen used is viable and has a high degree of germination (Kav et al, 2014). Success of artificial pollination with viable pollens is a crucial process in the production of date palm (Kadri et al, 2022).…”

Section: Resultsmentioning

confidence: 99%

“…Awad and Al-Qurashi (2012) reported comparable findings for spray pollination, which had a decreased FS. The observed differences in FS percentage between date palm cultivars in response to different pollination methods could be due to the variation in the pollination period following spathe cracking, which usually lasts up to 40 d and varies greatly depending on cultivar and climatic conditions, particularly temperature (Kadri et al, 2022). Generally, depending on the date palm cultivar, the length of the receptivity period of pistillate flowers can range from 3 to 10 d (8 d for Ghars and Jihel, 7 d for Deglet Nour, and %3 d for Boufeggous, Medjhool, and Iklane) (Shaheen et al, 1986).…”

Section: Resultsmentioning

confidence: 99%

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Innovative and Effective Spray Method for Artificial Pollination of Date Palm Using Drone

Alyafei

Dakheel

Almoosa³

et al. 2022

horts

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Traditional pollination of date palms is a time-consuming and labor-intensive process. Hence, the objectives of this study were 1) to develop a rapid, efficient, and low-cost pollination method that results in an acceptable level of fruit set (FS) with smaller amount of pollen grains, and labor costs using a platform-mounted robotic (drone) supplemented with water-suspended pollen grains, and 2) to investigate the effect of this method on date palm FS percentage, pollination efficiency (PE), fruit retention, total yield, and fruit quality. Date palm cultivars Barhi, Lulu, and Khesab were pollinated using traditional hand pollination (HP), spray pollination (HS), and drone pollination (DS) with water-suspended pollen grains (3 g/L). The results revealed that DS had a significantly lower FS percentage than the HP and HS methods in the Lulu and Khesab cultivars, but the difference was not statistically significant in Barhi cultivar. Fruit PE was unaffected by any of the pollination methods in Barhi and Lulu cultivars, but in the Khesab cultivar, DS pollination had lower PE (0.81) than HS (0.94) and HP (0.99) methods. DS had significantly lower levels of fruit retention and bunch weight than other methods. DS significantly improved fruit physical quality at harvest in all cultivars compared with HP and HS methods. Nevertheless, fruit color, firmness, total soluble solids (TSS) %, acidity, pH, and vitamin C level were unaffected by any of the pollination methods. Although the DS method produced lower FS percentage and bunch weight, the obtained FS percentages were within the commercially acceptable range for all cultivars. To the best of our knowledge, this is the first field study to indicate the possibility of pollinating date palm using drones with water-suspended pollen grain. To validate the commercial application, more research is needed to justify the optimum time and duration of application, which may vary according to cultivar and region.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Innovative and Effective Spray Method for Artificial Pollination of Date Palm Using Drone

Alyafei

Dakheel

Almoosa³

et al. 2022

horts

View full text Add to dashboard Cite

show abstract

“…Pollen preservation is important for producing new varieties through hybridization ( Báez et al., 2002 ; Kadri et al., 2022 ). Pollen longevity can be extended when it has a low moisture content and when stored at low temperatures ( Akond et al., 2012 ; Özcan, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Optimization of culture medium for in vitro germination and storage conditions of Exochorda racemosa pollen

Jia

Wang²,

Mi³

et al. 2022

Front. Plant Sci.

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Pollen morphology, pollen vigor, and long-term pollen storage are critical for plant cross-breeding and genetic improvement of Exochorda racemosa. We developed a protocol for viability determination and storage of E. racemosa pollen for breeding new varieties. The medium components for E. racemosa pollen germination was optimized by using an Orthogonal Assay Test Strategy (OATS). The germination rates of E. racemosa pollen were investigated after storing at different temperatures and different storage periods. The size of E. racemosa pollen was medium with three germination ditches, and the sculptural type of pollen was striate. Red ink and 2,3,5-triphenyl tetrazolium chloride (TTC) can effectively distinguish viable pollen from the unviable pollen of E. racemosa. The most suitable medium (CK2) for E. racemosa was composed of 150 g· L–1 sucrose, 100 mg·L–1 boric acid, 150 mg· L–1 Ca(NO3)2 and 50 mg· L–1 GA3. Low-temperature stress produced the greater inhibition of pollen tube growth compared with high-temperature conditions. The CK2 medium at pH 6.5 resulted in the highest pollen germination rate and most extended pollen tube length. The optimal temperature for storage of dried pollen was –80°C (P < 0.01), and the germination rate was 53.60% after storage for 390 days. Thawing in a 35°C water bath produced the best viability of E. racemosa pollen after storage at –20°C and –80°C. The short-term storage of E. racemosa fresh pollen at 4°C was better than that at –20°C and –80°C (P < 0.01). It is possible to evaluate pollen quality and store pollen grains for E. racemosa by the parameters defined in this study.

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“…Kadri found the best fruit set for date palm (Phoenix dactylifera L.) was achieved by pollination between 12:00 p.m. to 15:00 p.m. with fresh pollen performed on the same day of spathe cracking (Kadri et al, 2021), higher storage temperature and longer storage duration can decrease the fruit set rate to as 45% or less. Pitaya blooms at 8:00 p.m. and fades around 8:00 a.m. the next day.…”

Section: Introductionmentioning

confidence: 99%

Pollen germination and hand pollination in pitaya (Selenicereus spp.)

Shi

Dai

et al. 2022

Emir J Food Agric

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Hand pollination is a necessary assisting method for pitaya (Selenicereus spp.) production to achieve a high yield. With the cultivated area increasing at an exponential rate in recent years, a comprehensive study of the pollination process was conducted. We developed an ideal medium for pitaya pollen in vitro germination in this study, then tested the activity of pollen collected from or stored for various time periods. We discovered that those collected between 2 h before blooming and 6 h after blooming had the higher germination rates (27.2–65.1%), the highest activity was at 2 h after blooming, and that storing them at 4°C for 24 hours reduces their germination rate from 65.2 percent to 35.5 percent and their production to about 82%. As a result, pollinating plants with pollen that has been held for more than 24 hours is not recommended unless a breakthrough in pollen storage is achieved. We also discovered that stigma receptivity and pollen activity are synchronized, which together determines the rate of fruit setting and the size of the fruit. Pollination within 6 hours after flowering offers the optimum fruit setting percentage and size, while pollination at 6:00 pm, 2 hours before blooming, is also a good alternative; however, pollination at 6:00 am the next morning is expected to result in a 23 % drop in productivity. These findings will be beneficial for reproductive biology research, as well as laying the groundwork for hand pollination to boost pitaya output and breeding efficiency.

show abstract

Effect of pollination time, the hour of daytime, pollen storage temperature and duration on pollen viability, germinability, and fruit set of date palm (Phoenix dactylifera L.) cv "Deglet Nour"

Cited by 17 publications

References 9 publications

Innovative and Effective Spray Method for Artificial Pollination of Date Palm Using Drone

Innovative and Effective Spray Method for Artificial Pollination of Date Palm Using Drone

Optimization of culture medium for in vitro germination and storage conditions of Exochorda racemosa pollen

Pollen germination and hand pollination in pitaya (Selenicereus spp.)

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