Effect of Poly(N-vinyl-pyrrolidone)-block-poly(d,l-lactide) as Coating Agent on the Opsonization, Phagocytosis, and Pharmacokinetics of Biodegradable Nanoparticles

Abstract

The effect of the coating polymer poly(N-vinyl-pyrrolidone) (PVP) on the protein adsorption, phagocytosis, and pharmacokinetics of poly(D,L-lactide)-based nanoparticles was evaluated in vitro and in vivo. Control poly(ethylene glycol) (PEG)-coated nanoparticles were included for comparison. While no difference between PEG-and PVPdecorated nanoparticles in terms of amount of adsorbed protein was evident upon incubation in single protein solutions (BSA, IgG), incubation in serum revealed a protein adsorption pattern both quantitatively and qualitatively distinct. Larger amounts of complement components and immunoglobulins were found to adhere to PVP-coated particles, whereas PEG particles showed preferential adsorption of apolipoproteins. Furthermore, preopsonization in fresh rather than heat-inactivated serum enhanced uptake of both types of particles by murine RAW 264.7 macrophages. However, when isolated rat Kupffer cells were employed, activation of the complement system significantly enhanced the uptake of PVP-coated nanoparticles compared to PEG particles. Ultimately, PVPcoated nanoparticles exhibited considerably shorter circulation times compared to their PEG counterparts when administered intravenously to rats.

Protein corona: a new approach for nanomedicine design

“…The protein-NP interaction is studied by incubating NPs in protein solutions for a period of time, either a single protein solution such as BSA, fibronectin and IgG, [11][12][13][14] or biological fluid containing multiple proteins such as serum, 15,16 plasma, cytosol, 17 fetal bovine serum (FBS), 18 fetal calf serum, synovial fluid, and cerebrospinal fluid. 19 Protein-NP complexes are then separated from the protein solution via instrumental analysis.…”

Efficient delivery of ursolic acid by poly(N-vinylpyrrolidone)-block-poly (ε-caprolactone) nanoparticles for inhibiting the growth of hepatocellular carcinoma in vitro and in vivo

“…11 Recently, it was reported that the capability of PVP to prevent protein adsorption was correlated with its coating and molecule weight. 13,14 It was found that the nanoparticles coated by PVP, with molecular weight of 2,500 and 4,800 Da, were rapidly removed from blood due to complement components and immunoglobulins adsorption. 14 In this report, we loaded UA into poly(N-vinylpyrrolidone)-block-poly (ε-caprolactone) (PVP-b-PCL) nanoparticles and performed physiochemical characterization.…”

“…13,14 It was found that the nanoparticles coated by PVP, with molecular weight of 2,500 and 4,800 Da, were rapidly removed from blood due to complement components and immunoglobulins adsorption. 14 In this report, we loaded UA into poly(N-vinylpyrrolidone)-block-poly (ε-caprolactone) (PVP-b-PCL) nanoparticles and performed physiochemical characterization. In vitro and in vivo antitumor effects of the UA-loaded PVP-b-PCL nanoparticles (UA-NPs) were evaluated.…”

Polyethylene glycol gold-nanoparticles: Facile nanostructuration of doxorubicin and its complex with DNA molecules for SERS detection

“…As an example of biocompatible functionalization, polyethylene glycol (PEG) and poly N-vinyl-2-pyrrolidone (PVP) were exploited to modify nanovectors for gene drug/delivery [17][18][19] , tumor targeting 20,21 A c c e p t e d M a n u s c r i p t and x-ray imaging 22 . For in vivo applications using PEG-gold nanoparticles, all the preparations were based on conventional methods (i.e.sodium citrate or sodium borohydrate reduction) and post-addition of thiolated PEG leading to gold particles usually larger than 20 nm 23 .…”