Effect of polycaprolactone scaffolds containing different weights of graphene on healing in large osteochondral defect model

başal, özgür; Özmen, Özlem; Deliormanlı, Aylin M.

doi:10.1080/09205063.2022.2042035

Cited by 9 publications

(7 citation statements)

References 38 publications

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“…A possible explanation for this observation may be related to some GPN release from the scaffold in such a concentration that it impairs cell viability. However, in accordance with previous studies, the regular morphology and high proliferation/migration capacity demonstrated by the BM MSCs cultured on 3D PCL/GPN scaffolds (Figure 8) support their suitable application in tissue-engineering strategies, particularly focusing on bone regeneration [24,75,76].…”

Section: Discussionsupporting

confidence: 91%

Additive Manufactured Poly(ε-caprolactone)-graphene Scaffolds: Lamellar Crystal Orientation, Mechanical Properties and Biological Performance

et al. 2022

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Understanding the mechano–biological coupling mechanisms of biomaterials for tissue engineering is of major importance to assure proper scaffold performance in situ. Therefore, it is of paramount importance to establish correlations between biomaterials, their processing conditions, and their mechanical behaviour, as well as their biological performance. With this work, it was possible to infer a correlation between the addition of graphene nanoparticles (GPN) in a concentration of 0.25, 0.5, and 0.75% (w/w) (GPN0.25, GPN0.5, and GPN0.75, respectively) in three-dimensional poly(ε-caprolactone) (PCL)-based scaffolds, the extrusion-based processing parameters, and the lamellar crystal orientation through small-angle X-ray scattering experiments of extruded samples of PCL and PCL/GPN. Results revealed a significant impact on the scaffold’s mechanical properties to a maximum of 0.5% of GPN content, with a significant improvement in the compressive modulus of 59 MPa to 93 MPa. In vitro cell culture experiments showed the scaffold’s ability to support the adhesion and proliferation of L929 fibroblasts (fold increase of 28, 22, 23, and 13 at day 13 (in relation to day 1) for PCL, GPN0.25, GPN0.5, and GPN0.75, respectively) and bone marrow mesenchymal stem/stromal cells (seven-fold increase for all sample groups at day 21 in relation to day 1). Moreover, the cells maintained high viability, regular morphology, and migration capacity in all the different experimental groups, assuring the potential of PCL/GPN scaffolds for tissue engineering (TE) applications.

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Section: Discussionsupporting

confidence: 91%

Additive Manufactured Poly(ε-caprolactone)-graphene Scaffolds: Lamellar Crystal Orientation, Mechanical Properties and Biological Performance

et al. 2022

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“…PCL is preferred due to its low processing temperature, biocompatibility, and mechanical stability. 98 In the study by Basal et al, 35 graphene incorporated 3D printed PCL scaffolds were produced. A needle with a 410 μm diameter was used and printing was carried out with a printing speed of 12 mm/s and 3 bar air pressure.…”

Section: D Printed Polyester Scaffoldsmentioning

confidence: 99%

“…5,32−34 Moreover, cells are encapsulated in 3D printed scaffolds to enable more effective treatment of osteochondral defects. 35 In the studies on osteochondral research with 3D printing, the most exploited biomaterials were alginate, gelatin methacryloyl (GelMa), polyethylene glycol diacrylate (PEGDA)-based hydrogels, and polyester scaffolds (polycaprolactone (PCL), polylactic acid (PLA) and poly(lactideco-glycolide) (PLGA)). These common biomaterials are reinforced with other materials to further reinforce their physical and biological properties.…”

Section: Introductionmentioning

confidence: 99%

“…3D printing comes forth as a promising technology to print anatomically matched implants . In this way, the rigidity, diffusivity, and tissue density of articular cartilage may be more precisely mimicked. ,− Currently the three most popular 3D printing techniques of hydrogels are (i) extrusion-based (fused deposition modeling (FDM), low temperature deposition modeling (LDM)), (ii) vat-polymerization (stereolithography (SLA), digital light processing (DLP)), and (iii) jetting-based (electrohydrodynamic jet printer). ,− Moreover, cells are encapsulated in 3D printed scaffolds to enable more effective treatment of osteochondral defects …”

Section: Introductionmentioning

confidence: 99%

“… 5 , 32 − 34 Moreover, cells are encapsulated in 3D printed scaffolds to enable more effective treatment of osteochondral defects. 35 …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Is 3D Printing Promising for Osteochondral Tissue Regeneration?

Ege

Hasırcı²

2023

ACS Appl. Bio Mater.

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Osteochondral tissue regeneration is quite difficult to achieve due to the complexity of its organization. In the design of these complex multilayer structures, a fabrication method, 3D printing, started to be employed, especially by using extrusion, stereolithography and inkjet printing approaches. In this paper, the designs are discussed including biphasic, triphasic, and gradient structures which aim to mimic the cartilage and the calcified cartilage and the whole osteochondral tissue closely. In the first section of the review paper, 3D printing of hydrogels including gelatin methacryloyl (GelMa), alginate, and polyethylene glycol diacrylate (PEGDA) are discussed. However, their physical and biological properties need to be augmented, and this generally is achieved by blending the hydrogel with other, more durable, less hydrophilic, polymers. These scaffolds are very suitable to carry growth factors, such as TGF-β1, to further stimulate chondrogenesis. The bone layer is mimicked by printing calcium phosphates (CaPs) or bioactive glasses together with the hydrogels or as a component of another polymer layer. The current research findings indicate that polyester (i.e. polycaprolactone (PCL), polylactic acid (PLA) and poly(lactide-co-glycolide) (PLGA)) reinforced hydrogels may more successfully mimic the complex structure of osteochondral tissue. Moreover, more recent printing methods such as melt electrowriting (MEW), are being used to integrate polyester fibers to enhance the mechanical properties of hydrogels. Additionally, polyester scaffolds that are 3D printed without hydrogels are discussed after the hydrogel-based scaffolds. In this review paper, the relevant studies are analyzed and discussed, and future work is recommended with support of tables of designed scaffolds. The outcome of the survey of the field is that 3D printing has significant potential to contribute to osteochondral tissue repair.

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