Effect of polycation nanostructures on cell membrane permeability and toxicity

Wytrwał-Sarna, Magdalena; Knobloch, Paulina; Lasota, Sławomir; Michalik, Marta; Nowakowska, Maria; Kępczyński, Mariusz

doi:10.1039/d1en01156a

Cited by 16 publications

(13 citation statements)

References 41 publications

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“…Although cationic polymer-based delivery systems have been actively developed in recent decades, their toxicity hampers the clinical translation for tissue regeneration and disease treatment [ 36 , 40 ]. While the deleterious effects of polycations could damage cell membranes and induce apoptosis [ 41 , 42 ], suitable in vitro and in vivo biocompatibility of PEAD-based Coa has been demonstrated through a series of studies [ 14 , 15 , 36 ]. Interestingly, the administration of bolus AAL provided a more abundant dose of available AAL in the surrounding environment during the initial interaction with cancer cells, but facilitated cancer cell death was detected in mPEG-Coa-mediated AAL treatment.…”

Section: Discussionmentioning

confidence: 99%

Coacervate-mediated novel pancreatic cancer drug Aleuria Aurantia lectin delivery for augmented anticancer therapy

2022

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Background Pancreatic cancer, one of the cancers with the highest mortality rate, has very limited clinical treatment. Cancer cells express abnormal glycans on the surface, and some lectins with a high affinity for the glycans induce apoptosis in cancer. In this study, the efficacy of Aleuria Aurantia lectin (AAL) for the treatment of pancreatic cancer was evaluated and the efficacy improvement through AAL delivery with mPEGylated coacervate (mPEG-Coa) was investigated. Methods AAL was treated with pancreatic cancer cells, PANC-1, and the expression level of caspase-3 and subsequent apoptosis was analyzed. In particular, the anticancer efficacy of AAL was compared with that of concanavalin A, one of the representative anticancer lectins. Then, methoxypolyethylene glycol-poly(ethylene arginylaspartate diglyceride), a polycation, was synthesized, and an mPEG-Coa complex was prepared with polyanion heparin. The AAL was incorporated into the mPEG-Coa and the release kinetics of the AAL from the mPEG-Coa and the cargo protection capacity of the mPEG-Coa were evaluated. Finally, improved anticancer ability through Coa-mediated AAL delivery was assessed. Results These results indicated that AAL is a potential effective pancreatic cancer treatment. Moreover, mPEG-Coa rapidly released AAL at pH 6.5, an acidic condition in the cancer microenvironment. The initial rapid release of AAL effectively suppressed pancreatic cancer cells, and the continuous supply of AAL through the Coa transporter effectively inhibited proliferation recurrence of cancer cells. Conclusion AAL is a potential novel drug for the treatment of pancreatic cancer therapeutic agent. In addition, a continuous supply of drugs above the therapeutic threshold using mPEG-Coa could improve therapeutic efficacy.

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Section: Discussionmentioning

confidence: 99%

Coacervate-mediated novel pancreatic cancer drug Aleuria Aurantia lectin delivery for augmented anticancer therapy

2022

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“…10 Alterations in the structure and components of cell membranes are likely crucial factors that contribute to cellular responses induced by NPs, including but not limited to toxicological effects. 11 The complexity in membrane components and structures makes it almost impossible to understand the mechanism of NP–bio interactions by using cells themselves. Because synthetic small unilamellar vesicles (SUVs) and supported lipid bilayers (SLBs) make the structural and chemical control of model membranes much simpler, they are often used as model systems of cell membranes to study fundamental biophysical processes.…”

Section: Introductionmentioning

confidence: 99%

Continuous-time binding kinetics of graphene oxide quantum dots and lipid bilayers dominated by hydrogen bonding: effect of nanoparticles' protein corona and membrane components

Ren,

Wang,

et al. 2024

Environ. Sci.: Nano

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“…11 Thus, in situ characterizations of these three parameters allow the assessment of the metabolic and electrophysiological activities of the cardiac tissue. Various techniques have been applied for monitoring oxygen concentration around cells (such as a Clark-type electrode 12 ), characterization of membrane perme- ability (e.g., electron microscopy, 13 microplate reader, 14 and spectrophotometer 15 ), and measuring contraction behavior of cardiomyocytes (such as microelectrode array, 16 patch clamp, 17 and fluorescence imaging method 18 ). However, the metabolic and electrophysiological states of cardiac tissue change rapidly once applied with electric stimulation.…”

Section: ■ Introductionmentioning

confidence: 99%

Effect of Exogenous Electric Stimulation on the Cardiac Tissue Function In Situ Monitored by Scanning Electrochemical Microscopy

Zhao

et al. 2023

Anal. Chem.

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Cardiac tissue is sensitive to and can be easily damaged by exogenous electric stimulation. However, due to the thermal−electric coeffect and the limitation of in situ and quantitative information on the cardiac tissue function under electric stimulation, the detailed effect and the underlying mechanism of exogenous electric stimulation on the cardiac tissue remain elusive. To address this, in this work, we first constructed an in vitro cardiac tissue model and established a thermal−electric coupled theoretical model for simulating the electric field and temperature distributions around the cardiac tissue, from which we selected the electric field strengths (1.19, 2.37, and 3.39 kV cm −1 ) and electrical energies (0.001, 0.005, and 0.011 J) for electric stimulations without inducing a thermal effect. Then, we applied electric field stimulations on the cardiac tissue using these parameters and scanning electrochemical microscopy (SECM) to in situ and quantitatively monitor the dynamic changes in the key parameters of the cardiac tissue function, including respiratory activity, membrane permeability, and contraction frequency, after electric field stimulations. The SECM results showed that the oxygen consumption, cell membrane permeability coefficient, and contraction frequency of the cardiac tissue were strongly dependent on electrical energy, especially when the electrical energy was higher than 0.001 J. Our work, for the first time, achieves the in situ and quantitative monitoring of the cardiac tissue function under electric stimulation using SECM, which would provide important references for designing an electric stimulation regime for cardiac tissue engineering and clinical application of electrotherapy.

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Effect of polycation nanostructures on cell membrane permeability and toxicity

Abstract: The interaction of nanometric synthetic materials with cell membranes is one of the key factors determining their possible cytotoxicity. This work investigated the interaction of polycation nanostructures with lipid and...

Cited by 16 publications

References 41 publications

Coacervate-mediated novel pancreatic cancer drug Aleuria Aurantia lectin delivery for augmented anticancer therapy

Coacervate-mediated novel pancreatic cancer drug Aleuria Aurantia lectin delivery for augmented anticancer therapy

Continuous-time binding kinetics of graphene oxide quantum dots and lipid bilayers dominated by hydrogen bonding: effect of nanoparticles' protein corona and membrane components

Effect of Exogenous Electric Stimulation on the Cardiac Tissue Function In Situ Monitored by Scanning Electrochemical Microscopy

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