Effect of polymorphisms in the FASN, OLR1, PPARGC1A, PRL and STAT5A genes on bovine milk‐fat composition

Abstract

The aim of our study was to estimate effects of polymorphisms in the ATP-binding cassette G2 (ABCG2), fatty acid synthase (FASN), oxidized low-density lipoprotein receptor 1 (OLR1), peroxysome proliferator-activated receptor-gamma coactivator-1alpha (PPARGC1A), prolactin (PRL) and signal transducer and activator of transcription 5A (STAT5A) genes on milk production traits and detailed milk-fat composition. Milk-fat composition phenotypes were available for 1905 Dutch Holstein-Friesian cows. First, the presence of each SNP in the Dutch Holstein-Friesian population was evaluated by direct sequencing of the PCR product surrounding the SNP in 22 proven Dutch Holstein-Friesian bulls. The ABCG2 SNP did not segregate in the bull population. Second, we genotyped the cows for the FASN(g.16024G>A), FASN(g.17924A>G), OLR1(g.8232C>A), PPARGC1A(c.1790+514G>A), PPARGC1A(c.1892+19G>A), PRL(g.8398G>A) and STAT5A(g.9501G>A) polymorphisms, and estimated genotype effects on milk production traits and milk-fat composition. FASN(g.17924A>G) and OLR(g.8232C>A) had a significant effect (P < 0.05) on milk-fat percentage. However, we were not able to confirm results reported in the literature that showed effects of all evaluated polymorphisms on milk-fat percentage or milk-fat yield. All polymorphisms showed significant effects (P < 0.05) on milk-fat composition. The polymorphisms in FASN and STAT5A, which had an effect on C14:0 and were located on chromosome 19, could not fully explain the quantitative trait locus for C14:0 that was previously detected on chromosome 19 in a genome-wide scan using linkage analysis.

Genotype and allele frequencies of polymorphisms in ABCG2, PPARGC1A and OLR1 genes in indigenous cattle breeds in Turkey

“…In our study, CC genotype at the c.3359T>C locus, was found at very high levels as 0.65 and 0.80 in SAR and EAR, respectively while no TC heterozygote genotype was found in neither breed. Although our fi ndings are in agreement with those genotype frequencies reported by Weikard [6,8,18,21]. Unlike the fi ndings of Kowalewska-Luczak et al (2010) in the Jersey breed, AC genotype at the c.3359A>C locus was found at the highest level in SAR and EAR [20].…”

“…Similarly, Kowalewska -Luczak et al (2010) did not fi nd any relation between c.1892T>C and c.3359A>C polymorphisms of PPARGC1A gene and milk yield traits in Jersey cattle [20]. In the previous studies, the highest genotype frequency for CC genotype was found 0.68 in German Holstein [6], 0.53 in Polish Holstein-Friesian cattle [8], 0.56 in German Holstein-Friesian [21], 049 in Chinese Holstein cattle [18] while CT genotype was found at the highest frequency of 0.65 in the University of Wisconsin dairy herd [7], and 0.72 in Jersey breed [20]. Kowalewska-Luczak et al (2010) found the frequency of PPARGC1A gene c.3359A>C polymorphism genotype AA to bethe highest in the Jersey breed cattle while they did not fi nd genotype CC [20].…”

Association of bovine PPARGC1A and OPN genes with milk production and composition in Holstein cattle

“…The highest allele frequency was reported for C allele in this study and other researches (White et al, 2007;Komisarek and Dorynek 2009;Schennink et al 2009). The frequencies were reported in c.3359A>C position of PPARGC1A gene as follows: the highest frequency was found for the AC genotype and the lowest frequency for the CC genotype.…”

“…Weikard et al (2005) showed that cows with the TT genotypes of c.1892T>C position yielded higher milk fat than the other Kowalewska-Luczak et al (2010) in Jersey cows. Schennink et al (2009) found an association between c.1892T>C and milk fat composition in Dutch Holstein-Friesian cattle. Significant relationships were found between the c.8514C>T genotypes and FAT P2X and PRO PER305 traits (Table 2).…”

Genetic effects of ABCG2 polymorphism on milk production traits in the Chinese Holstein cattle

“…Single nucleotide polymorphisms (SNPs) in the diacylglycerol acyltransferase 1 (DGAT1), stearoyl-CoA desaturase 1(SCD1), fatty acid synthase (FASN), oxidised low-density lipoprotein receptor 1 (OLR1), prolactin (PRL), signal transducer activator of transcription 5A (STAT5A) and growth hormone receptor (GHR) genes have been shown to affect the composition of bovine milk in different cattle populations (Mele et al 2007;Moioli et al 2007;Schennink et al 2008Schennink et al , 2009Sun et al 2009). Polymorphisms in several other genes that play a role in metabolism pathways have been associated with milk production and composition.…”