Nowadays, the need for biobased materials is inevitable due to the effective implementation of sustainability concepts. Bamboo fibers have great potential to widen their application range due to their low density and high strength properties. Hence, this study investigates the chemical, mechanical, thermal, and morphological characteristics of untreated and alkaline (NaOH) treated bamboo fibers extracted from bambusa bamboo Indian species. Different instrumental methods, such as thermogravimetric analysis (TGA), X-ray diffraction (XRD), universal tensile testing, and scanning electron microscopy (SEM), have been used for the testing of bamboo fiber samples in both conditions. The result of the analysis reveals that the reduction of hemicellulose content was observed between 18.5 and 17.5% in the case of alkali-treated conditions. More importantly, alkali treatment increased the thermal stability due to the exposure of strong cellulose components on the fiber surface. The decreasing aspect ratio after the removal of unwanted layers could be the reason for the improved tensile strength of the treated bamboo fiber. A significant improvement in the crystallinity index (broad band width of the second peak) and thermal resistance was attained in the alkali-treated condition, which is commendable in comparison with some other natural fibers. Based on its performance, chemically treated bamboo fiber can be used to develop structural members in the automobile and construction industries.