Effect of Pomegranate Peel Extract on Submandibular Salivary Glands of Streptozotocin-Induced Diabetes in Rats:  Histological, Immunohistochemical and Ultrastructural Study

Mahmoud, Elham F.; Mahmoud, Mahmoud F.

doi:10.9734/jabb/2017/34101

Cited by 9 publications

(8 citation statements)

References 30 publications

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“…Regarding the immunohistochemical analysis of Bcl-2 protein expression in submandibular salivary glands, this work revealed that sections of rats in (C) group that were exposed to normal saline or in (Se) group exhibited strong cytoplasmic patterns expression in the stromal cells between acini of the salivary glands, while those of rats of (CY) group showed mild or negative cytoplasmic patterns expression in the stromal cells between acini. These findings were in accordance with another study showed the anti-apoptotic effect of selenium by increasing the BCL-2 protein expression [34].…”

Section: Discussionsupporting

confidence: 93%

The role of Selenium in Mitigating The adverse Effect of Cyclophosphamide on The rat Submandibular Salivary Glands

Alnuaimi

Mammdoh

Allaf

2022

Egyptian Journal of Veterinary Sciences

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T HE goal of this study is to see if selenium can counteract the effects of cyclophosphamide on albino rats' submandibular salivary gland tissues. 24 male rats were separated into four groups, each with 6 rats aged 12 -16 weeks weighing 300-400 grams: the first was given physiological saline orally, the second was given selenium (200 mg/kg) orally for 14 days, and the third group was given cyclophosphamide orally once (150 mg /kg). On day 8, group 5 th group(IV) got selenium (200 Mg/kg) and cyclophosphamide (150 mg/kg) intraperitoneally (i.p.). On day 15, all rats were sedated and slaughtered, and the salivary glands of the submandibular region were collected. In comparison to the other groups, the third group lost weight significantly. The histopathological study revealed mucosal acini necrosis with edema surrounding the striated ducts, as well as mucosal acini cell atrophy and vacuoles in the serous acini. A decrease in the number of granulocytic convoluted tubules, an increase in fibrous tissue surrounding the interlobular ducts, and serous acinar apoptosis characteristics were also seen. In comparison to the other groups with moderate to severe foci, immunohistochemical results in this group demonstrated modest to the negative expression of (anti-apoptotic Bcl-2 protein) in stromal cells among the acinar cells of the salivary glands. We concluded that cyclophosphamide caused structural alterations in the submandibular salivary glands of rats and that selenium alleviated these side effects, as seen by histological investigation and immunohistochemistry analysis of Bcl-2 expression.

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Section: Discussionsupporting

confidence: 93%

The role of Selenium in Mitigating The adverse Effect of Cyclophosphamide on The rat Submandibular Salivary Glands

Alnuaimi

Mammdoh

Allaf

2022

Egyptian Journal of Veterinary Sciences

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“…In the present study the stem cell treated group III showed a statistically significant strong positive immunoreaction of Bcl-2 with mean value (38.84±3.904) in submandibular gland tissue, this finding was in accordance with Mahmoud, Mahmoud, 2017 (42) . This finding confirms the antiapoptotic effect of MSCs where these cells have been observed to increase the antiapoptotic Bcl-2 protein expression and suppress the apoptotic The various effects of MSCs appeared to occur through a paracrine signalling pathways in the surrounding environment and also through direct cell communication with the formation of intercellular nanotubes with surrounding cells (44).…”

Section: Discussionsupporting

confidence: 89%

Impact of Bone Marrow Stem Cells Application on the Methotrexate Induced Submandibular Salivary Gland Degenerative Changes in Albino Rats (Histological and Immuno-Histochemical Study)

Emam

Salam

Ghazy

2019

Egyptian Dental Journal

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Introduction: cytotoxic drugs produce a structural changes in salivary glands that results in disturbances in their function. Patients treated with these cytotoxic drugs complain from xerostomia that results in difficulties in swallowing, speech, taste sensation, oropharyngeal pain and oral infections. Aim: This is study was conducted to evaluate the effect of local injection of bone marrow stem cells on submandibular glands of rats receiving Methotrexate as chemotherapeutic drug histologically and immunohistochemically. Materials and Methods:Thirty male adult albino rats (150-200 gm). They were randomly divided into three groups (n=10). Group I: rats were received single intraperitoneal injection of Phosphate buffered saline PBS 1 ml at the day one of the experiment. Group II: rats received single intraperitoneal injection of 40 mg/kg Methotrexate (MTX) dissolved in 1ml saline at day one of the experiment. Groups III: rats were handled as those in group II; however, rats were subjected to intra glandular injection of bone marrow stem cells (BMMSCs) suspended in 0.1 ml PBS 7 days after the start of the experiment at both sides of submandibular salivary gland (SMG). Animals of all groups were euthanized at three weeks after the start of experiment and two pairs of (SMG) were dissected out, processed and the prepared sections were examined histologically by H&E, immunohistochemical stain for Bcl-2 and morphomtrical analysis. Data obtained from immunohistochemical analysis were statistically described in terms of mean ± standard deviation (± SD).Results: Histological examination of Group I revealed the normal histological structure of the gland and mild positive immunoreaction of Bcl-2. Group II has glandular disorganization. The nuclei of the acinar cells revealed different sizes and shape (polymorphism) and negative immunoreactivity for Bcl-2. Group (III) revealed marked improvement in cells of acini as well as cells of ducts lining and the acini relatively preserved their shape with strong cytoplasmic (1752)

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“…during the first night after induction to avoid sudden hypoglycemic post [22]. Mice were declared diabetic if on the 7th day after induction of blood sugar levels ≥ 200 mg / dL checked using Accu check (0197, EasyTouch GCU, Bioptik Technology Inc., Taiwan) [22,23].…”

Section: Diabetic Rats Modelmentioning

confidence: 99%

Regeneration of Salivary Gland Defects of Diabetic Wistar Rats Post Human Dental Pulp Stem Cells Intraglandular Transplantation on Acinar Cell Vacuolization and Interleukin-10 Serum Level

Narmada

Laksono

Nugraha

et al. 2019

Pesqui. bras. odontopediatria clín. integr.

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Objective: To investigate the regeneration of rat's salivary gland diabetic defect after intraglandular transplantation of Human Dental Pulp Stem Cells (HDPSCs) on acinar cell vacuolization and Interleukin-10 (IL-10). Material and Methods: HDPSCs isolated from the dental pulp of first premolars #34. HDPSCs from the 3rd passage was characterized by immunocytochemistry of CD73, CD90, CD105 and CD45. Twenty-four male Wistar rats, 3month-old, 250-300 grams induced with Streptozotocin 30 mg/kg body weight to create diabetes mellitus (DM) divided into 4 groups (n=6); positive control group on Day-7; positive control group on Day-14; treatment group Day-7 (DM+5.105HDPSCs); treatment group on Day-14. On Day-7 and Day-14, rats were sacrificed. Histopathological examination performed to analyze acinar cells vacuolization while Enzyme-linked Immunoabsorbent Assay to measure IL-10 serum level. Data obtained were analyzed statistically using multiple comparisons Bonferroni test, Kruskal Wallis, Shapiro-Wilk and Levene's test result. Results: The highest acinar cell vacuolization found in control group Day 14 (0.239 ± 0.132), meanwhile the lowest acinar cell vacuolization found in treatment group Day 7 (0.019 ± 0.035) with significant difference (p=0.003). The highest IL-10 serum level found in treatment group Day 14 (175.583 ± 120.075) with significant difference (p=0.001). Conclusion: Transplantation of HDPSC was able to regenerate submandibular salivary gland defects in diabetic rats by decreasing acinar cell vacuolization and slightly increase IL-10 serum level.

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Effect of Pomegranate Peel Extract on Submandibular Salivary Glands of Streptozotocin-Induced Diabetes in Rats: Histological, Immunohistochemical and Ultrastructural Study

Cited by 9 publications

References 30 publications

The role of Selenium in Mitigating The adverse Effect of Cyclophosphamide on The rat Submandibular Salivary Glands

The role of Selenium in Mitigating The adverse Effect of Cyclophosphamide on The rat Submandibular Salivary Glands

Impact of Bone Marrow Stem Cells Application on the Methotrexate Induced Submandibular Salivary Gland Degenerative Changes in Albino Rats (Histological and Immuno-Histochemical Study)

Regeneration of Salivary Gland Defects of Diabetic Wistar Rats Post Human Dental Pulp Stem Cells Intraglandular Transplantation on Acinar Cell Vacuolization and Interleukin-10 Serum Level

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