Effect of potassium iodide on growth of Sporothrix schenckii in yeast form

Bareja, Rajesh; Grover, Prem Singh; Mehra, S K

doi:10.5455/spatula.20160414060306

Cited by 3 publications

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“…On the seventh day the suspension of YNB medium with S. schenckii was adjusted to 90% transmission at 540 nm on the photo-colorimeter. 4 Master culture thus prepared was used for subsequent analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Potassium iodide (KI) has been used in the treatment of sporotrichosis, however, the exact mechanism of action remains unknown. 4 Therefore, it was planned to undertake the present study to estimate the transaminases enzymes in the mycelial form of S. schenckii and to study the effect of KI with increasing concentration on the production of these enzymes in-vitro.…”

Section: Introductionmentioning

confidence: 99%

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Expression of transaminase enzymes and effect of potassium iodide on its production in mycelial form of Sporothrix schenckii

Bareja¹,

Shinu²

2021

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Background : Sporotrichosis is chronic, pyogranulomatous fungal infection of cutaneous or subcutaneous. It is caused by the dimorphic fungus Sporothrix schenckii (S. schenckii). Aims: This study describes the in-vitro effect of potassium iodide (KI) on the transaminases enzymes, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) produced by the filamentous form of S. schenckii. Methods and Material: A master culture of S. schenckii was prepared in YNB (Yeast nitrogen base) medium and was incubated at 25ºC (mould). KI was added into the YNB medium in increasing concentrations. One mL suspension of master culture was inoculated into each bottle and incubated at 25ºC for different time period, 4 th day (early-log period), 9 th day (mid-log period) and 14 th day (peak of growth) respectively. After centrifuging, a 5% homogenate was prepared that was used for transaminases enzyme assay. Results: The mean aspartate aminotransferase level of control specimen was 12.4 ± 3.30, 22.4 ± 3.69 and 53.6 ± 8.46 IU on day 4, 9 and 14 respectively. The mean aspartate aminotransferase level of test specimen was ranged from 3.5 ± 0.80 (KI 6.4 gram %) to 12.4 ± 4.66 IU (KI 0.1 gram %), 2.6 ± 0.21 (KI 6.4 gram %) to 29.5 ± 7.31 IU (KI 0.05 gram %) and 2.5 ± 0.23 (KI 6.4 gram %) to 42.3 ±3.70 IU (KI 0.2 gram %) on day 4, 9 and 14 respectively. The mean alanine aminotransferase level of control specimen was 17.5 ± 5.93, 24.6 ± 3.59 and 32.6 ± 7.54 IU on day 4, 9 and 14 respectively. The mean alanine aminotransferase level of test specimen was ranged from 2.2 ± 0.00 (KI 6.4 gram %) to 15.4 ± 2.36 IU (KI 0.1 gram %), 2.7 ± 0.81 (KI 6.4 gram %) to 29.5 ± 2.75 IU (KI 1.6 gram %) and 3.5 ± 1.37 (KI 6.4 gram %) to 29.6 ± 2.82 IU (KI 0.2 gram %) on day 4, 9 and 14 respectively. Conclusions: At the entire test concentrations mean value was lower as compared to control. The low activity of the transaminases enzymes indicates that KI has inhibitory effect on the growth of S. schenckii (mould) and has led to decrease in the activity of these enzymes.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression of transaminase enzymes and effect of potassium iodide on its production in mycelial form of Sporothrix schenckii

Bareja¹,

Shinu²

2021

IJMMTD

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“…A master culture was prepared by doing the subculture of S. schenckii from slope of Sabouraud's dextrose agar (SDA) in 50 mL of YNB (Yeast nitrogen base, HiMedia, Mumbai) medium that was incorporated in a screw-capped bottle and was incubated at 37ºC. On day seven, growth of S. schenckii in the bottle was adjusted to 90% transmission at 540 nm by adding the appropriate amount of YNB medium as described by Bareja et al 2 Master culture thus prepared was used for subsequent analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Sporotrichosis (mainly cutaneous and lymphocutaneous sporotrichosis) caused by Sporothrix schenckii (S. schenckii) when it penetrates through the broken skin and differentiates into the yeast form within the infected tissue. 1,2 A number of drugs and other therapies have been recommended for the treatment of sporotrichosis. These include Potassium Iodide (KI), itraconazole, fluconazole, terbinafine, amphotericin B and thermotherapy.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of acid phosphatase enzyme and influence of potassium iodide on its production in the yeast form of Sporothrix schenckii

2019

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Background: Sporotrichosis is caused by a dimorphic fungal species, Sporothrix schenckii (S. schenckii). The enzyme acid phosphatase is pervasive among yeast and yeast like fungi. It has been studied in various fungi like Aspergillus oryzae, Candida albicans etc. but in S. schenckii little is known about enzyme acid phosphatase. The present study depicts the in-vitro influence of Potassium Iodide (KI) on the enzyme acid phosphatase produced by the S. schenckii (yeast form).Methods: A master culture was prepared by incorporating the standard strain of S. schenckii in YNB (Yeast Nitrogen Base) medium and was incubated at 37ºC. After preparing the increasing concentrations with KI in YNB medium, 1.0 mL suspension of master culture was inoculated into each bottle and incubated at 37ºC for different time period 6th, 12th, 18th day (early, mid, peak of log period) respectively. After centrifuging, a 5% homogenate was prepared, which was used for acid phosphatase enzyme assay.Results: The mean acid phosphatase level of control specimen was 20.9±2.01, 50.0±2.25, 45.0±5.10 μg and test specimens was ranged from 14.9±4.89 to 20.2±3.49, 10.2±4.19 to 40.0±6.39 and 10.0±1.81 to 34.7±6.08 μg on day 6, 12 and 18 respectively. The mean value was lower significantly for all the test concentrations as compared to control (p<0.05).Conclusions: The low activity of the enzyme acid phosphatase indicates that KI has inhibitory effect on the growth of S. schenckii that has led to decrease in the activity of the enzyme.

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Assessment of transaminase enzymes and effect of potassium iodide on its production in the yeast form of Sporothrix schenckii

Bareja,

Sumedha,

Pottathil

2024

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The dimorphic fungus () is the cause of sporotrichosis. Many fungi have transaminases, also known as aminotransferases, which are portions of proteins. However, nothing is known about this enzyme inThe current study shows how potassium iodide (KI) affects the transaminases enzymes aspartate aminotransferase (AST) and alanine aminotransferase (ALT) produced by yeast form in vitro.YNB (yeast nitrogen base) medium was used to create a master culture of, which was then incubated at 37ºC (yeast). The amount of KI added to the YNB medium increased. Each container received one millilitre (mL) of the master culture suspension, which was then incubated at 37ºC for different lengths of time – the early-log phase on day six, the mid-log period on day twelve, and the growth peak on day eighteen, respectively. A 5% homogenate was produced after centrifugation and used in the transaminases enzyme assay.On days 6, 12, and 18, the control specimen's mean aspartate aminotransferase level was 10.11 ± 3.09, 10.36 ± 2.33, and 17.62 ± 4.27 IU, respectively. On days 6, 12, and 18, the test specimen's mean aspartate aminotransferase level ranged from 9.80 ± 2.42 (KI 0.4 gramme %) to 19.59 ± 3.9 IU (KI 0.2 gramme %), from 4.52 ± 2.28 (KI 0.4 gramme %) to 28.46 ± 4.88 IU (KI 0.2 gramme %), and from 4.50 ± 1.02 (KI 0.8 gramme %) to 14.49 ± 3.60 IU (KI 0.05 gramme %). On days 6, 12, and 18, the control specimen's mean alanine aminotransferase level was 10.70 ± 3.82, 29.60 ± 3.02, and 19.74 ± 4.62 IU, respectively. Day 6, 12, and 18 saw variations in the test specimen's mean alanine aminotransferase level, which ranged from 11.40 ± 3.04 (KI 0.1 gramme %) to 18.52 ± 3.97 IU (KI 0.2 gramme %), 7.82 ± 1.50 (KI 0.8 gramme %) to 41.56 ± 4.56 IU (KI 0.2 gramme %), and 3.33 ± 0.70 (KI 0.8 gramme %) to 12.54 ± 1.92 IU.The transaminase enzymes' low activity indicates that KI has an inhibiting effect on the growth of (yeast), which has caused a drop in the enzymes' activity.The impact of KI on the lipids of may be monitored to comprehend the mechanism of action of KI in the future.

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Effect of potassium iodide on growth of Sporothrix schenckii in yeast form

Cited by 3 publications

References 0 publications

Expression of transaminase enzymes and effect of potassium iodide on its production in mycelial form of Sporothrix schenckii

Expression of transaminase enzymes and effect of potassium iodide on its production in mycelial form of Sporothrix schenckii

Assessment of acid phosphatase enzyme and influence of potassium iodide on its production in the yeast form of Sporothrix schenckii

Assessment of transaminase enzymes and effect of potassium iodide on its production in the yeast form of Sporothrix schenckii

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