Field experiment was conducted for two consecutive years from kharif 2015 and summer 2017at research farm of Division of Agronomy, Skuast Jammu to study the effect of differential substitution of nutrients through organics on yield, nitrogen uptake and balance sheet of nitrogen of four crops basmati rice (1st crop) - knolkhol (2nd crop) - knolkhol (3rd crop) - green gram (4th crop) in cropping sequence for two years in Irrigated subtropical conditions of Jammu and Kashmir. The result of two year studied showed that significantly highest yield of all the four crops in two crop cycles in the rice based cropping system viz., basmati rice, knolkhol-1, knolkhol-2 and green gram were recorded with 100 % recommended dose of fertilizer which was found statistically at par with yields recorded with treatments 75 % NPK +25 % N through vermicompost and Fym (1:1),25% yearly replacement of RDF through vermicompost and Fym (1:1) on N basis,75% NPK+25% N through vermicompost,25 % yearly replacement of RDF through vermicompost on N basis,75 % NPK+ 25 % N through Fym and 25% yearly replacement of RDF through Fym on N basis. Further, highest mean total nitrogen uptake (544.95 kg/ha) in rice based cropping system were recorded with treatment 25% yearly replacement of RDF through Fym on N basis followed by treatment 75 % NPK+ 25 % N through Fym (541.29 kg/ha) whereas highest build-up of available nitrogen in soil after two crop cycles (8.79 kg/ha) was recorded with treatment 100 % N through Farmyard manure followed by treatment 100% N through Vermicompost whereas lowest build-up of available nitrogen (4.53 kg/ha) in soil was recorded with treatment 100 % Recommended dose of fertilizer in rice based cropping system.