Effect of praziquantel on the strobilar development of <i>Mesocestoides corti</i> in vitro

Britos, Leticia; Domínguez, Laura; Ehrlich, Ricardo; Marı́n, Mónica

doi:10.1017/s0022149x00000433

Cited by 23 publications

(15 citation statements)

References 16 publications

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“…There are some examples of agents that stimulate cestode larval development while reversibly disrupt tegument. That is the case of PKC activators in Schistosoma mansoni (Wiest et al, 1994), praziquantel in M. corti (Britos et al, 2000), and N-lauroyl sarcosinate in Mesocestoides vogae (Hrckova et al, 1998), which concomitantly increased larvae segmentation rates and induced morphological damage on their surfaces. Likewise, bile salts, such as sodium taurocholate, stimulate cestode development (Smyth and Davies, 1974;Esch and Smyth, 1976;Kawamoto et al, 1986;Saldana et al, 2001Saldana et al, , 2003.…”

Section: Discussionmentioning

confidence: 99%

Early post-larval development of the endoparasitic platyhelminthMesocestoides corti: Trypsin provokes reversible tegumental damage leading to serum-induced cell proliferation and growth

et al. 2005

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Mesocestoides corti is a suitable in vitro model for studying the development of human endoparasitic platyhelminthes. Treatment with trypsin, supplemented with fetal bovine serum (FBS), induces M. corti development from larvae (tetrathyridia) to segmented adult worm; however, the role of this protease and of FBS in post-larval development induction remains unknown. To characterize the participation of trypsin enzymatic activity and of FBS in the induction of tetrathyridia growth and development, both stimuli were added to the larvae either together or sequentially. Additionally, specific inhibition of trypsin activity was also monitored. Finally, the effect of the enzyme on the parasite tegument as well as the proliferative activity and location of proliferating cells after induction of tetrathyridia development were also studied. We conclude that trypsin-induced tetrathyridia development to adult worm is FBS-dependent and that the effect of serum factors is dependent upon a previous trypsin-induced reversible damage to the larva tegument. In dividing and non-dividing tetrathyridia, proliferative activity of cells is mainly located within the apical massif in the anterior region and nerve cords of larvae, respectively. In tetrathyridia stimulated to develop to adult worms, an intense proliferative activity is evident along the nerve cords. Our results suggest that in natural infections the tetrathyridia tegument is temporally made permeable to growth factors by proteolytic enzyme activity in the intestine juice of the definitive host, thus leading to development to adult worms.

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Section: Discussionmentioning

confidence: 99%

Early post-larval development of the endoparasitic platyhelminthMesocestoides corti: Trypsin provokes reversible tegumental damage leading to serum-induced cell proliferation and growth

et al. 2005

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“…Parasite removal and in vitro culture was performed as described by Britos et al . [48]. Briefly, approximately 500 μl of tetrathyridia were cultured in 15 ml of modified RPMI 1640 with 10% bovine fetal serum.…”

Section: Methodsmentioning

confidence: 99%

Stem cell proliferation during in vitro development of the model cestode Mesocestoides corti from larva to adult worm

et al. 2010

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BackgroundIn free-living flatworms somatic differentiated cells do not divide, and a separate population of stem cells (called neoblasts) is responsible for cell proliferation and renewal. In cestodes, there is evidence that similar mechanisms of cell renewal exist.ResultsIn this work, we have characterized proliferative cells during the development of the model cestode Mesocestoides corti from larva (tetrathyridium) to young segmented worm. This was done by two complementary strategies with congruent results: characterizing cells in S phase and their progeny by incorporation of 5-bromo-2'-deoxyuridine, and characterizing cells in M phase by arresting mitotic cells with colchicine and studying their morphology and distribution. Proliferative cells are localized only in the inner parenchyma, particularly in close proximity to the inner muscle layer, but not in the cortical parenchyma nor in the sub-tegumental tissue. After proliferation some of these cells migrate to the outer regions were they differentiate. In the larvae, proliferative cells are more abundant in the anterior regions (scolex and neck), and their number diminishes in an antero-posterior way. During the development of adult segments periodic accumulation of proliferative cells are observed, including a central mass of cells that constitutes the genital primordium, which grows at least in part due to in situ proliferation. In later segments, the inner cells of genital primordia cease to proliferate and adopt a compact distribution, and proliferative cells are also found in the testes primordia.ConclusionsProliferative cells have a characteristic localization and morphology throughout development from larva to adult of Mesocestoides corti, which is similar, and probably evolutionary conserved, to that described in other model cestodes. The characteristics of proliferative cells suggest that these consist of undifferentiated stem cells.

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“…Parasite removal and culture were made following Britos et al (2000). Tetrathyridia were cultured in vitro to obtain segmented worms, showing an elongate body and numerous proglottids, and separated manually.…”

Section: Methodsmentioning

confidence: 99%

Hox Genes in the Parasitic Platyhelminthes Mesocestoides corti, Echinococcus multilocularis, and Schistosoma mansoni: Evidence for a Reduced Hox Complement

2009

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Little is known about the Hox gene complement in parasitic platyhelminthes (Neodermata). With the aim of identifying Hox genes in this group we performed two independent strategies: we performed a PCR survey with degenerate primers directed to the Hox homeobox in the cestode Mesocestoides corti, and we searched genomic assemblies of Echinococcus multilocularis and Schistosoma mansoni. We identified two Hox genes in M. corti, seven in E. multilocularis, and nine in S. mansoni (including five previously reported). The affinities of these sequences, and other previously reported Hox sequences from flatworms, were determined according to phylogenetic analysis, presence of characteristic parapeptide sequences, and unusual intron positions. Our results suggest that the last common ancestor of triclads and neodermatans had a Hox gene complement of at least seven genes, and that this was probably derived by gene loss from a larger ancestral Hox complement in lophotrochozoans.

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Effect of praziquantel on the strobilar development of Mesocestoides corti in vitro

Cited by 23 publications

References 16 publications

Early post-larval development of the endoparasitic platyhelminthMesocestoides corti: Trypsin provokes reversible tegumental damage leading to serum-induced cell proliferation and growth

Early post-larval development of the endoparasitic platyhelminthMesocestoides corti: Trypsin provokes reversible tegumental damage leading to serum-induced cell proliferation and growth

Stem cell proliferation during in vitro development of the model cestode Mesocestoides corti from larva to adult worm

Hox Genes in the Parasitic Platyhelminthes Mesocestoides corti, Echinococcus multilocularis, and Schistosoma mansoni: Evidence for a Reduced Hox Complement

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