Effect of pre-milking antiseptic treatment on the bacterial contamination of cow teats’ skin

Abstract

SummaryMišeikienė, R., J. Rudejevienė & G. Gerulis, 2015. Effect of pre-milking antiseptic treatment on the bacterial contamination of cow teats' skin. Bulg. J. Vet. Med., 18, No 2,[159][160][161][162][163][164][165][166] The primary objective of premilking udder preparation and teats antiseptic is to achieve an acceptable level of decontamination of teat skin. This aids in reducing the spread of microorganisms and incidence of new intramammary infections, and in minimising the number of bacteria. The aim of our studies was to investigate the influence of pre-milking teat antiseptic solutions on total bacterial contamination of teat skin. Three udder antiseptics were applied: Dermisan 0.5% (active ingredientaminopropyl laurylamine), 0.2% solution with active ingredient iodine, and foaming solution of natural compounds (lactic acid+glycerol+allantoin). Cow teats were swabbed before and after application of udder preparations. The total bacterial contamination on cows teat skin was determined employing serial dilutions and plate count method. Microorganisms were identified under uniform conditions on McConkey (for Gram-negative bacteria), Columbia blood agar medium (for staphylococci), containing 5% sheep's blood, Edwards (for streptococci) agars. The statistical analysis of the data was performed using descriptive statistics and independent-sample T test procedures in SPSS 13.0 for Windows. The udder applications with lactic acid and iodine had the highest probability (P<0.05) of reducing total bacterial contamination. The use of udder antiseptics for premilking teats preparation reduced the levels of coliforms, coagulase negative staphylococci and Streptococcus uberis but with exception of iodine, no effect was found on reducing Candida genus yeasts.

A review of test protocols for the evaluation of teat disinfectants

“…(), Mišeikienė et al . () and Baumberger et al . () used a teat swabbing protocol to determine the efficacy of a range of premilking teat disinfectants.…”

“…) or performed by triple rotary motions around the surface of the teat close to the tip from the front teat only (Mišeikienė et al . ). Different types of media have been used to store the sample swabs: 4 mL of buffered peptone water (Baumberger et al .…”

“…Swab samples are usually diluted to 10 −2 to 10 −3 for postdisinfectant swab samples (Mišeikienė et al . ; Baumberger et al . ) and 10 −4 for predisinfectant swab samples (Baumberger et al .…”

“…Mišeikienė et al . () used the teat swabbing protocol to evaluate a 0.2% w/w iodine disinfectant, 0.5% w/w aminopropyl‐laurylamine disinfectant and a lactic acid‐based disinfectant. The authors concluded that lactic acid and iodine had the highest probability of reducing total bacterial contamination, compared to 0.5% w/w aminopropyl‐laurylamine.…”

Effect of premilking teat sanitation on milk composition, somatic cell count and test day milk yield in Holstein cows

“…Reports have emphasized that good hygiene practices before the milking times are powerful processes to remove microorganisms from the teat-skin area and to obtain raw milk with low SCC (Gibson et al, 2008;Sandrucci et al, 2014). In normal, microorganisms enter raw milk either from the environment of cow's or from the udder (Miseikiene et al, 2015). That's why, proper milking hygiene practice is a key subject for ensuring high quality raw milk.…”