Effect of pre-treatment of sperm with progesterone and cholesterol on in-vitro embryo production in cattle

Minireview: Functions of the cumulus oophorus during oocyte maturation, ovulation, and fertilization

“…Progesterone for instance is present in the medium after IVM and IVF of bovine COCs (Tanghe et al, 2001). It has been suggested that progesterone is a possible inducer of acrosome reaction in humans (Bronson et al, 1999), cattle (Giritharan et al, 1999), horses (Cheng et al, 1998), guinea pigs (Jia et al, 1997), hamsters (Llanos and Anabalon, 1996), and mice (Herrero et al, 1997). Progesterone interacts with speci®c sperm binding sites located on the sperm plasma membrane and induces a dramatic increase in cytosolic Ca 2 that leads to membrane fusion.…”