Effect of Pre-treatment Solution on Iris hollandica Flower: Vase life, Anthocyanin Content, and Peroxidase Activity

Abstract

<jats:p><jats:italic>Iris hollandica</jats:italic> `Blue Magic' was treated with deionazed water as a control, 3% sucrose (Suc), 3% sucrose plus 0.4 m<jats:sc>m</jats:sc> silver thiosulphate (Suc+STS), 3% sucrose plus 200 mg·L<jats:sup>-1</jats:sup> 8-hydroxyquinoline sulphate (Suc+HQS) and 3% sucrose plus 100 mg·L<jats:sup>-1</jats:sup> benzyl amino-purine (Suc+BA) for 4hrs and then transferred to tap water. The vase life treated with Suc+BA was extended 4 days longer than that of control. The treatment Suc+STS or Suc+HQS did not improve vase life. The amounts of water uptake and transpiration by all treatments decreased after harvest, but those values were higher in cut iris treated with Suc+BA than in those with control. Cut flowers treated with by Suc+BA markedly improved water balance, comparing with control which was quickly changed to minus value. Anthocyanin content in petals of cut flower treated with Suc+BA was 3.5 fold higher than that of control. The treatment by Suc+BA delayed discoloration in petals and senescence of cut Iris. Peroxidase (POD) activities of all treatments were reached maximum at 4th day after treatment and decreased thereafter. POD activity was highest when the cut iris was treated with Suc+BA. These results show that the use of Suc+BA is most effective treatment for improving the vase life and quality of cut Iris flowers.</jats:p>

Androgenesis in tomato (Solanum lycopersicum L.)-Effect of genotypes, microspore development stage, pre-treatments and media composition on induction of haploids

“…Similar results were observed in pepper (Capsicum annuum L.) where embryogenesis was improved and one to three plants per 100 anthers were obtained when 4°C temperature for 48 h was applied as a pre-treatment to flower buds (Thriveni et al, 2020). However, a different kind of observation was also documented in pepper and there were no significant effects of cold pre-treatment on flower buds (Vagera & Havranek, 1985;Kim et al, 2004;Jha et al, 2021) The results suggested that various genotypes had different requirement of nutrients and growth regulators. The highest callus induction frequency for anthers from different size of fresh flower buds for different genotypes were TA8(MS + 2iP (0.5 mg L -1 ) + NAA (0.5 mg L -1 )) for LE 1230 and PKM1, TA1(MS + 2iP (1.0 mg L -1 ) + IAA (2.0 mg L -1 )) for CO 3, LE 1238 and LE 1256 and TA7 (MS + 2iP (0.5 mg L -1 ) + Kinetin (1.5 mg L -1 ) + NAA (1.0 mg L -1 )) for TLCV2 and the least was always recorded in TA0 (MS basal).…”

“…According to Supena et al (2006) and İlhan and Kurtar (2022), the determining factor for successful anther culture is the selection of flower buds containing over 50 per cent microspores in the late-uninucleate phase. However, the early binucleate microspore stage is also amenable to androgenesis induction (Kim et al, 2004(Kim et al, , 2008González-Melendi et al, 2005). Kotyal et al (2022) reported that anthers containing over 75 per cent microspores in early binucleate phase were optimal for embryo production in isolated microspore cultures.…”

Effects of Pretreatment of NaOCl, Sucrose, and Benzyladenine on Vase Life and Quality of Cut Flower in Standard Chrysanthemum ‘Jinba’

“…Sucrose is widely used as a floral preservative. When 3% sucrose in cut rose and iris 'Blue Magic' (Bang et al, 2002;Kim et al, 2005), 10% sucrose in cut sweet pea and Lilium elegans (Ichimura and Hiraya, 1999;, and 20% sucrose in Agapanthus orientalis and Iris 'Discovery' (Macnish et al, 2010;Mor et al, 1984) were treated, quality and vase life of cut flower were effectively improved.…”