Effect of Pregnancy Sera on Isolated Lysosomes

Hempel, Karl; Fernandez, Linda A.; Persellin, Robert H.

doi:10.1038/225955a0

Cited by 37 publications

(17 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…0.1 ml of serum was mixed with 0.8 ml of the intact lysosome suspension before the addition of 0.1 ml of 0.1%o Triton X-100 and the absorbance change measured. This method for the detection of serum membrane reactants that modify organelle permeability has been previously described (9)(10). A lysosomal stabilizer in the serum will retard the rate of absorbance change followed addition of the membranolytic detergent.…”

Section: Methodsmentioning

confidence: 99%

Effects of Steroid Hormones on Human Polymorphonuclear Leukocyte Lysosomes

Persellin¹,

Ku²

1974

J. Clin. Invest.

Self Cite

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Effects of Steroid Hormones on Human Polymorphonuclear Leukocyte Lysosomes

Persellin¹,

Ku²

1974

J. Clin. Invest.

Self Cite

View full text Add to dashboard Cite

“…In separate experiments we have demonstrated that isolated lysosomes were protected from Triton X-100 lysis by pregnancy serum (38). This humoral stabilizing activity retarded the release of lysosomal enzymes and was shown to act on the membrane of the subcellular organelles.…”

Section: Discussionmentioning

confidence: 89%

Studies Of The Effects Of Pregnancy Serum On Polymorphonuclear Leukocyte Functions

Persellin

Leibfarth

1978

Arthritis & Rheumatism

Self Cite

View full text Add to dashboard Cite

The phagocytosis of both viable and heat-killed Staphylococcus aureus by normal human neutrophils was diminished in the presence of pregnancy serum.Particle ingestion was reduced significantly ( P < 0.0025) after 5 minutes of incubation when leukocytes were in 15% serum obtained during the latter phases of gestation. Control sera from normal adult females or males or from cord blood all functioned normally in support of phagocytosis. Intracellular metabolic events occurring after ingestion, including bacterial killing and nitroblue tetrazolium reduction, were decreased in proportion to the number of particles ingested. Differential studies involving leukocytes obtained from either preg-

show abstract

“…Since then such activity has been reported in granuloma pouch exudate in intact and adrenalectomized rats [2] arthritic and normal rat plasma [3,4] human pregnancy serum [5] anti-lymphocytic serum [6] human peritoneal fluid [7] extracts from regenerating rat liver damaged in vivo by dimethylnitrosamine (DMNO) [8] sponge-induced exudates in rat [9] and in human rheumatoid synovial fluid [10].…”

Section: Introductionmentioning

confidence: 99%

Putrescine — a potent endogenous anti-inflammatory substance in inflammatory exudates

1983

View full text Add to dashboard Cite

Sponge-induced exudate from the rat and saline extracts of normal rat liver and rat liver damaged in situ by dimethylnitrosamine have been assayed for putrescine, spermidine and spermine levels. The three physiological oligoamines have been examined for anti-inflammatory activity by the carrageenan-induced oedema rat model and spermidine and putrescine were found to be anti-inflammatory with putrescine being about ten times as active as spermidine. Putrescine was also anti-inflammatory in the adjuvant arthritic rat model. Thioacetamide and theophylline were also anti-inflammatory and theophylline doubled the level of putrescine in rat liver. It was concluded that putrescine was a potent anti-inflammatory factor in the inflammatory exudates and extracts examined.

show abstract

Effect of Pregnancy Sera on Isolated Lysosomes

Cited by 37 publications

References 7 publications

Effects of Steroid Hormones on Human Polymorphonuclear Leukocyte Lysosomes

Effects of Steroid Hormones on Human Polymorphonuclear Leukocyte Lysosomes

Studies Of The Effects Of Pregnancy Serum On Polymorphonuclear Leukocyte Functions

Putrescine — a potent endogenous anti-inflammatory substance in inflammatory exudates

Contact Info

Product

Resources

About