Effect of preparation conditions on the nanostructure of hydroxyapatite and brushite phases

Mansour, S.F.; El-Dek, S.I.; Ahmed, Mohamed; Abdelwahab, Sherif; Ahmed, M. K.

doi:10.1007/s13204-015-0509-4

Cited by 88 publications

(25 citation statements)

References 39 publications

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“…The reduction in theoretical density calculated by x-ray diffraction means that the arrangement of atoms in the unit cell tends to be loose and irregular, and the interaction between the atoms is weakened, the reduction in density indicates less stability. This is consistent with the result that the x-ray diffraction peaks broadened and shifted [14][15][16][17]. Quantitative experiments were further conducted for structural analysis.…”

Section: Resultssupporting

confidence: 86%

“…-CO 3 2 -containing BCP and pure BCP from fish bone To measure the phase composition and crystal structure of the inorganic components in the prepared materials, slow-scan fullname (XRD) (D8 ADVANCE, Germany) was used to determine the exact peak positions, and the working conditions were as follows: Cu-Kα as the radiation source and scanning at a step of 0.03°and a rate of 4°/min in the angular range of 10-60°. Using SGI workstation Ccrius2 (version 4.2) software package of American MSI Corporation and DWBS941l software to refine the powder XRD analysis data of the two samples by Rietveld refinement, the unit cell parameters of the two groups of samples were obtained and the unit cell structure integrity was compared, both samples have a two-phase structure, the theoretical density was determined using the formula = Dx ZM NV (where M is the molecular weight of the sample, N is Avogadro's number, Z is the number of molecules per unit cell and V is the unit cell volume) calculate the density of each single phase of each sample, and then calculate the calculated density of the two samples according to the formula = + Dx AD BD x x 1 2 (where A, B are the relative contribution ratios of the existing crystalline phases) [14][15][16][17]. The molecular functional groups of the sample, especially the -CO 3 2 vibration, were analyzed by fullname (FTIR) spectroscopy (1750, Perkin-Elmer, USA).…”

Section: The Characterization Ofmentioning

confidence: 99%

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Design and construction of a microporous CO 3 2 − -containing HA/β-TCP biphasic ceramic as a novel bone graft material

Dou

Chen

et al. 2020

Mater. Res. Express

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Biphasic calcium phosphate (BCP) ceramics, viewed as the first-line therapeutic materials in clinical practice, are still confronted with challenges such as undesirable biocompatibility and bioactivity given their structural and chemical deficiency. In this study, we designed a novel microporous -CO 3 2 -containing BCP ceramic from Salmo salar bone in a CO 2 atmosphere via two-step hightemperature sintering, and its physicochemical properties and biocompatibility were explored. A without -CO 3 2 -BCP bioceramic material prepared in a single gas atmosphere was used as a comparison. The two scaffolds were characterized by x-ray diffraction (XRD), fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM) , measured the size of its porosity and specific surface area by an instrument and in vitro cell experiment was used to test its biocompatibility. Such synthetic strategy endowed the BCP products with increased β-tricalcium phosphate (β-TCP) percentage from 37.18wt% to 45.3wt% (compared to BCP without -CO 3 2 ), with the -CO 3 2 content calculated to be 1.98wt%, which is close to that in human bone tissue. The amount of -CO 3 2 incorporation, the a-axes of the lattice parameters have decreased significantly from 9.4845 nm to 9.2942 nm by Rietveld structure refinement. Especially, the novel -CO 3 2 containing BCP exhibits a lower degree of crystallinity than that without CO3 2-. In addition, the natural microporosity remarkably increased from 49.6% for BCP without -CO 3 2 to 64.06% for -CO 3 2 -containing BCP, and the specific surface area increased from 5.829 m 2 g −1 to 17.161 m 2 g −1 . Besides, two materials were non-toxic to human bone marrow mesenchymal stem cells (hBMSCs), and the -CO 3 2 -containing BCP had more factor expression in the early stage of osteogenesis induction, such as Runx2 and Smad 4. Overall, microporous -CO 3 2 -containing BCP were prepared via a simple preparation method from natural bone, combining the structural and chemical preponderance, as well as in vitro performance it should be considered as a promising biomaterial for further explorations in the fields of bone graft materials from natural bone to satisfy versatile clinical requirements.

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Section: Resultssupporting

confidence: 86%

Section: The Characterization Ofmentioning

confidence: 99%

Design and construction of a microporous CO 3 2 − -containing HA/β-TCP biphasic ceramic as a novel bone graft material

Dou

Chen

et al. 2020

Mater. Res. Express

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“…Bone’s apatite is not a pure HAP; it is naturally doped with different elements and concentrations 3 . Synthesized HAP is distinguished by its good biocompatibility, osteoconductivity and bone-attachment ability but its brittleness, rigidity and high degradation rate have limited HAP from being used in the broad spectrum of biomedical applications 4 5 6 7 .…”

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confidence: 99%

Physico-mechanical and morphological features of zirconia substituted hydroxyapatite nano crystals

Mansour

El-Dek

Ahmed

2017

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141

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Zirconia doped Hydroxyapatite (HAP) nanocrystals [Ca10(PO4)6−x(ZrO2)x(OH)2]; (0 ≤ x ≤ 1 step 0.2) were synthesized using simple low cost facile method. The crystalline phases were examined by X-ray diffraction (XRD). The crystallinity percentage decreased with increasing zirconia content for the as-synthesized samples. The existence of zirconia as secondary phase on the grain boundaries; as observed from scanning electron micrographs (FESEM); resulted in negative values of microstrain. The crystallite size was computed and the results showed that it increased with increasing annealing temperature. Thermo-gravimetric analysis (TGA) assured the thermal stability of the nano crystals over the temperature from room up to 1200 °C depending on the zirconia content. The corrosion rate was found to decrease around 25 times with increasing zirconia content from x = 0.0 to 1.0. Microhardness displayed both compositional and temperature dependence. For the sample (x = 0.6), annealed at 1200 °C, the former increased up to 1.2 times its original value (x = 0.0).

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“…The theoretical density (Dx) was calculated from XRD data using the following formula :

Dx = ZM / NV

where Z : the number of molecules per unit cell, M : the molecular weight of the sample, N : Avogadro's number, and V the unit cell volume calculated from XRD data.…”

Section: Methodsmentioning

confidence: 99%

Fe³⁺/⁻ dual doped nano hydroxyapatite: A novel material for biomedical applications

et al. 2017

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Dual ions substituted hydroxyapatite (HA) received attention from scientists and researchers in the biomedical field owing to their excellent biological properties. This paper presents a novel biomaterial, which holds potential for bone tissue applications. Herein, we have successfully incorporated ferric (Fe 31 )/selenate (SeO 22 4 ) ions into the HA structure (Ca 10-x-y Fe y (PO 4 ) 6-x (SeO 4 ) x (OH) 2-x-y O y ) (Fe-SeHA) through a microwave refluxing process. The Fe-SeHA materials were characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and field emission scanning electron microscopy (FESEM). XRD and FTIR analyses revealed that Fe-SeHA samples were phase pure at 9008C. FESEM images showed that formation of rod-like shaped particles was inhibited dramatically with increasing Fe 31 amount. The Vickers hardness (HV) test showed that hardness values increased with increasing Fe 31 concentrations. Optical spectra of Fe-SeHA materials contained broadband over (200-600) nm. In vitro degradation and bioactivity tests were conducted in simulated body fluid (SBF). The incorporation of Fe 31 /SeO 22 4 ions into the HA structure resulted in a remarkably higher degradation rate along with intense growth of apatite granules on the surface of the Fe-SeHA discs with Ca/P ratio of 1.35-1.47. In vitro protein adsorption assay was conducted in fetal bovine serum (FBS) and it was observed that the adsorption of serum proteins on Fe-SeHA samples significantly increased with increasing Fe 31 concentration. In vitro cytotoxicity tests were performed with human fetal osteoblast (hFOB)

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Effect of preparation conditions on the nanostructure of hydroxyapatite and brushite phases

Cited by 88 publications

References 39 publications

Design and construction of a microporous CO 3 2 − -containing HA/β-TCP biphasic ceramic as a novel bone graft material

Design and construction of a microporous CO 3 2 − -containing HA/β-TCP biphasic ceramic as a novel bone graft material

Physico-mechanical and morphological features of zirconia substituted hydroxyapatite nano crystals

Fe³⁺/⁻ dual doped nano hydroxyapatite: A novel material for biomedical applications

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Effect of preparation conditions on the nanostructure of hydroxyapatite and brushite phases

Cited by 88 publications

References 39 publications

Design and construction of a microporous CO 3 2 − -containing HA/β-TCP biphasic ceramic as a novel bone graft material

Design and construction of a microporous CO 3 2 − -containing HA/β-TCP biphasic ceramic as a novel bone graft material

Physico-mechanical and morphological features of zirconia substituted hydroxyapatite nano crystals

Fe3+/− dual doped nano hydroxyapatite: A novel material for biomedical applications

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Fe³⁺/⁻ dual doped nano hydroxyapatite: A novel material for biomedical applications