Effect of pressure on the integrity of the duct-acinar system of the pancreas

Pirola, Romano C.

doi:10.1136/gut.11.1.69

Cited by 28 publications

(1 citation statement)

References 9 publications

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“…These pressures have been closely studied in different species (man, dog, rat, cat) by several investigators;47 median values with the ranges during basal fasting conditions are bile duct 8cmH2O, [4][5][6][7][8][9][10][11][12][13][14][15][16][17], pancreatic duct 12cmH2O and duodenum 8cmH2O [4][5][6][7][8][9][10][11][12]. Although there is considerable variation between animals of the same species and even the same animal tested on different days, the pressure in the, pancreatic ducts is, in most instances, greater than that in the biliary system.…”

Section: Importantmentioning

confidence: 99%

Pressure, volume and the pancreas.

Armstrong¹,

Taylor²,

Torrance³

1985

Gut

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for eight hours, were carefully weighed and anaesthetised with 1*2 mg intraperitoneal sodium pentobarbitone, and maintained at a constant temperature of 37°C. Through a midline laparotomy wound the duodenum and bile-pancreatic duct were identified. In the rat multiple pancreatic ducts drain into a common bile-pancreatic duct which empties into the second part of the duodenum.9 10 The greater part of the bile-pancreatic duct is enveloped in pancreas but at each end there is an exposed segment 1-2 mm long allowing free access to the duct. The common bile duct was ligated close to the liver and the animals left for 30 minutes to enable the bile-pancreatic duct to clear of bile. A preliminary experiment on 10 animals showed that bile duct ligation did not affect pancreatic structure or function over a 24 hour period. Through a small duodenotomy a thin Portex polyethylene cannula (id 0-4mm, od 0-8mm) was inserted through the ampulla into the bile-pancreatic duct and tied in place ( Fig. 1) with care taken to avoid any pancreatic damage. The cannula was connected to a three way tap; one arm attached to a constant infusion pump (Slow infusion apparatus, Scientific

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Section: Importantmentioning

confidence: 99%

Pressure, volume and the pancreas.

Armstrong¹,

Taylor²,

Torrance³

1985

Gut

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Effects of bile, infection and pressure on pancreatic duct integrity

Armstrong

Taylor

Torrance

1985

Journal of British Surgery

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Ionic flux, potential difference and mucosal ultrastructure have been studied in the rat bile-pancreatic duct and the effects of pressure, bile and infection on the duct evaluated. The duct remained stable after perfusion with control solution under low pressure and high pressure produced widening of intercellular spaces only. Perfusion with a bacterial solution of Escherichia coli did not effect significant changes. Sterile human bile disturbed the integrity of the duct by increasing ionic flux, altering potential difference and producing reversible ultrastructural changes of cell oedema. High pressure increased these changes. Infected human bile under high or low pressure was by far the most toxic substance tested. Perfusion with infected bile led to irreversible duct damage and complete loss of duct integrity. Pressure and infected bile may have a role in damaging duct integrity and could thus play an integral part in the genesis of acute gallstone pancreatitis.

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Pancreatic duct pressure, duct permeability and acute pancreatitis

Harvey

Wedgwood

Austin

et al. 1989

Journal of British Surgery

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The relationship between pancreatic duct pressure, duct permeability to macromolecules and the development of acute pancreatitis was studied in a cat model. Perfusion of the pancreatic duct with 15 mM glycodeoxycholic acid, ethanol administration, or secretagogue-stimulated pancreatic secretion against greater than 50 per cent duct obstruction resulted in an increase in peak pancreatic duct pressure in all animals. Duct permeability to 20,000 molecular weight dextran molecules was increased in 22 of 29 experimental animals compared with two of 22 control animals (P less than 0.01). Perfusion of the pancreatic duct with activated pancreatic enzymes resulted in acute pancreatitis in 24 of 29 experimental animals compared with three of 22 control animals (P less than 0.01). These results suggest that pancreatic ductal hypertension, resulting in increased ductal permeability to large molecules, may be a common early event in gallstone and alcoholic pancreatitis.

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Effect of pressure on the integrity of the duct-acinar system of the pancreas

Cited by 28 publications

References 9 publications

Pressure, volume and the pancreas.

Pressure, volume and the pancreas.

Effects of bile, infection and pressure on pancreatic duct integrity

Pancreatic duct pressure, duct permeability and acute pancreatitis

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