Effect of pretreatment with artichoke extract on carbon tetrachloride-induced liver injury and oxidative stress

Mehmetçik, Güldal; Özdemirler, Gül; Koçak‐Toker, Necla; Çevikbaş, Uğur; Uysal, Müjdat

doi:10.1016/j.etp.2008.04.014

Cited by 91 publications

(66 citation statements)

References 28 publications

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“…dose of CCl 4 at 1.5 mg/kg administration caused very severe acute liver damage in rats, demonstrated by excessive elevation in serum ALT and AST activities as well as histopathological findings. This elevation of the activities of ALT and AST are consistent with the findings of Mehmetçik et al [26] which is similar acute CCl 4 toxicity study. Pretreament with A. lividus at 250 and 500 mg/kg and silymarin for 9 days prior to the CCl 4 administration, efficiently prevented the CCl 4 induced elevation in the serum ALT and AST activities and TBil levels which suggest that A. lividus maintains the stabilization of plasma membrane and thus protects the liver against CCl 4 induced damage.…”

Section: Histopathological Observationssupporting

confidence: 81%

Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats

Yilmaz-Ozden

2015

Turk J Bioch

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March 5, 2015] and evaporated to dryness under reduced pressure in a rotary evaporator (Buchi R210, Switzerland). The water extract of A. lividus was yielded a dark-brown solid residue, weighing 26 g (26%, w/w) which was stored at -20 o C. A. lividus is generally cooked with water before being consumed, for this reason water extract of A. lividus was used in this study. AnimalsMale albino Wistar rats (180-280 g) were obtained from the Institute of Experimental Medicine of Istanbul University and acclimatized to their environment for one week prior to experimentation. The animals were housed in an air-conditioned room with a 12 h light/dark cycle at controlled temperature and humidity conditions and supplied with standard laboratory diet and tap water ad libitum. The experimental procedure used in this study was approved by the Animal Assays Ethics Committee of Istanbul University (No:18527/16.07.2007). TreatmentsThe water extracts of A. lividus at 250 and 500 mg/kg [9] and the standard hepatoprotective drug silymarin (25 mg/ kg) [10] were administered orally as a fine suspension in carboxymethyl cellulose (CMC, 0.1%, w/v). These solutions were freshly prepared at each day of process. Liver damage was induced by intraperitoneal (i.p.) administration of a single dose of an equal mixture of CCl 4 and olive oil (1.5 mL/kg) [11]. The CCl 4 mixture was prepared immediately before treatment.Male albino Wistar rats were randomly divided into 7 groups. Group I, II and III served as control groups (n=5) which received orally CMC (4 mL/kg), A. lividus (500 mg/kg) and silymarin (25 mg/kg), respectively, daily for 9 days. Group IV, V, VI and VII served as experimental groups (n=7) which received orally CMC (4 mL/kg), A. lividus (250 mg/kg), A. lividus (500 mg/kg) and silymarin (25 mg/kg), respectively, daily for 9 days. On the 10th day, rats in the experimental groups (IV-VII) were treated with CCl 4 (1.5 mL/kg, 1:1 in olive oil, i.p.) while rats in the control groups (I-III) were treated with olive oil (1.5 mL/kg, i.p.). 24 h after CCl 4 administration, the animals were anesthetized with diethyl ether and sacrificed by collecting blood via cardiac puncture. Blood was allowed to coagulate for 30 min and serum was separated by centrifugation at 1,016 x g for 5 min at 4 o C. Serum was kept at -85 o C until it was used in further biochemical assays. Livers were quickly excised and washed in 0.9% NaCl to remove as much blood as possible and then tissue samples were immediately frozen at -85 o C for later use. The right upper lobe of the liver of each animal was used for the biochemical and histopathological analysis. Serum biochemical assayAlanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were assayed in the serum

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Section: Histopathological Observationssupporting

confidence: 81%

Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats

Yilmaz-Ozden

2015

Turk J Bioch

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“…The third group received orally liquid form of artichoke leaf extract at 1.5 g/kg dose for 14 d. The last group received single dose of 3 mg/kg alphaamanitine ip and then they were given liquid form of artichoke leaf extract at 1.5 g/kg dose for 14 d. All groups were decapitated under anesthesia at the end of day 14. Toxicity formation and administration route of the extract were defined according to previous studies in the literature [12][13][14] .…”

Section: Methodsmentioning

confidence: 99%

Alfa-Amanitin İle Oluşturulmuş Karaciğer Hasari Üzerine Enginar (Cynara scolymus) Sulu Yaprak Ekstresinin Etkileri

Kaymaz¹,

Kandemir²,

Pamukçu³

et al. 2016

Kafkas Univ Vet Fak Derg

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“…In addition, Mehmetcik et al [46] indicate that phytochemicals in grains and fruits have obtained great attention for their potential roles in human disease. Phenolics were considered as a major group of compounds that contribute to that antioxidant activity.…”

Section: Liver Enzyme Activitymentioning

confidence: 99%

The Potential Effect of Special Formulas on Cirrhotic Rats

Hassan¹,

El-Kherbawy²,

Ali³

et al. 2013

FNS

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Liver protective effect of special formulas (1 and 2) was assessed against carbon tetra chloride (CCl 4 ) which induced liver damage in Wister albino rats. The two prepared formulas reduced the changes in body weight and liver weight caused by CCl 4 in rats. The toxicity of CCl 4 is related to loss in body weight and increase in liver weight in rats. The weight ratios of liver to body weight (LW/BW) significantly increased in rats treated with CCl 4 followed by other groups. Formulas 1 and 2 could play an important role in improvement of hematological indices in liver cirrhosis rats. Feeding treated rats on special formulas showed improvement in liver function compared to rats fed on basal diet, reflected by significant reduction of the activity of transaminases (ALT and AST), alkaline phosphatase (ALP) and total bilirubin. There was a significant increase in total protein, albumin and globulin in serum. Significant increase in liver weight in rats treated with CCl 4 . There are no histopathological changes in all groups under study except for group 4 (CCl 4 treated rats fed on basal diet) which orally administrated with CCl 4 and had congestion of central vein and hepatic sinusoids.

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Effect of pretreatment with artichoke extract on carbon tetrachloride-induced liver injury and oxidative stress

Cited by 91 publications

References 28 publications

Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats

Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats

Alfa-Amanitin İle Oluşturulmuş Karaciğer Hasari Üzerine Enginar (Cynara scolymus) Sulu Yaprak Ekstresinin Etkileri

The Potential Effect of Special Formulas on Cirrhotic Rats

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