Effect of pretreatment with different MMP inhibitors on shear bond strength of composite to primary teeth dentin after six months of aging

Abstract

Tell: +98-9171016736Effect of pretreatment with different MMP inhibitors on shear bond strength of composite to primary teeth dentin after six months of aging Abstract Background: loss of hybrid layer integrity compromises the resin-dentin bond stability. Subsequent release and activation of matrix metalloproteinase enzymes during dental restorative procedures or caries development are contributing factors to dentin-adhesive bond failure. Aim:To investigate the effect of pre-treatment with different MMP inhibitors on the shear bond strength (SBS) of an etch-and-rinse adhesive (Adper Single Bond 2) to primary teeth dentin after six months of aging.Methods: Sixty extracted anterior primary teeth ,due to orthodontic reasons ,were selected. A dentin block (6.0 mm × 6.0 mm × 2.0 mm) was obtained from each tooth. All the dentin blocks (n = 60) were pretreated for 60 s with either 0.01 M phosphate buffered saline (PBS, pH 7.2) in the control group, 2% : Chlorhexidine (CHX) solution, 2% Doxycycline (DO)solution and EDTA 17% after etching and before applying etch-and-rinse adhesive system (Adper Single Bond 2). Then composite was applied in clear teflon cylinders . The specimens were stored in artificial saliva for 6 months at 37ºC and then the SBS values were determined with a universal testing machine .Representative samples were selected for visualization of the failure mode under stereomicroscope and SEM.Results: No statistically significant difference observed between the SBS of the EDTA group, DO group and the control group (P value>0.05). CHX showed significantly higher SBS values compared to the other groups of the study after six months(P value=0.011). Conclusion:Chlorhexidine significantly preserved the SBS of composite resin to deciduous dentin using etch and rinse adhesive Adper Single Bond 2 after 6 months of aging in artificial saliva.

Modern Adhesive Systems and Techniques of Their Application for Direct Restoration of Hard Dental Tissues Defects

“…Незважаючи на багаторічну історію розвитку АС та реставраційних матеріалів після відновлення зубів нерідко реєструють ускладнення у вигляді підвищеної чутливості, запалення пульпи, виникнення вторинного карієсу, порушення крайового прилягання та фіксації фотокомпозита. Причинами таких випадків вважають недотримання протоколів адгезивної підготовки, надмірне потрапляння вологи на тверді тканини зубів або, навпаки, їх пересушування, усадку пломбувальних матеріалів, руйнівну дію ферментів дентину, бактерій, слини на гібридний шар [2,3,4].…”

“…Despite the many-year history of developing AS and restorative materials, complications in the form of increased sensitivity, pulp inflammation, secondary caries, impaired marginal fit and photocomposite fixation are often registered after tooth restoration. The reasons for such cases are non-compliance with adhesive preparation protocols, excessive ingress of moisture on the hard tissues of the teeth or, on the contrary, their drying, shrinkage of filling materials, the destructive effect of dentin enzymes, bacteria, saliva on the hybrid layer [2,3,4].…”