Effect of primary and secondary Fasciola gigantica infection on specific IgG responses, hepatic enzyme levels and weight gain in buffaloes

Wang, Jinhui; He, Kangxin; Zhendi, Wang; Wen, Chongli; Han, Xiao-Ge; Meng, Zhen; Yuan, Xiang-Xiang; Wu, Zhengjiao; Zhang, Weiyu; Di, Wenda

doi:10.1007/s00436-022-07519-2

Cited by 4 publications

(3 citation statements)

References 28 publications

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“…Here, seven cytokines were investigated, and no significant differences in secretion between primary and secondary infection were detected in all weeks tested. Furthermore, autopsies of buffaloes with primary and secondary infections indicated no significant difference in the fluke recovery rate between these groups ( 13 ). Combined with the dynamics of the cytokines, this verifies that the challenge infection could not induce resistance against F. gigantica in buffaloes.…”

Section: Discussionmentioning

confidence: 99%

“…ITT sheep acquire resistance to F. gigantica both in primary and secondary infection (12). Previous laboratory research found that buffaloes were susceptible to primary and secondary infection by F. gigantica 4 weeks after primary infection, as the flukes recovery rate was similar in primary and secondary infection (means of 21.2 and 23.5% burden, respectively) (13). Thus, it can be inferred that buffaloes are susceptible to secondary infection with F. gigantica, and that secondary infection at the fourth week cannot induce the robust adaptive immune response.…”

Section: Introductionmentioning

confidence: 98%

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Secondary infection of Fasciola gigantica in buffaloes shows a similar pattern of serum cytokine secretion as in primary infection

et al. 2023

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BackgroundAs a natural host of Fasciola gigantica, buffalo is widely infected by F. gigantica. Its impact on buffalo production has caused great losses to the husbandry sector, and repeat infection is non-negligible. In buffaloes experimentally infected with F. gigantica, primary and secondary infection have yielded the same rate of fluke recovery, indicating a high susceptibility of buffalo to F. gigantica, which contributes to the high infection rate. Determining the immunological mechanism of susceptibility will deepen the understanding of the interaction between F. gigantica and buffalo. Here, we explored the immune response of buffaloes against primary and secondary F. gigantica infection, with a focus on cytokines’ dynamics explored through serum cytokine detection.MethodsBuffaloes were assigned to three groups: group A (noninfected, n = 4), group B (primary infection, n = 3), and group C (secondary infection, n = 3). Group B was infected via oral gavage with 250 viable F. gigantica metacercariae, and group C was infected twice with 250 metacercariae at an interval of 4 weeks. The second infection of group C was performed simultaneously with that of group B. Whole blood samples were collected pre-infection (0 weeks) and at 1–6, 10, and 12 weeks after that. The serum levels of seven cytokines (IFN-γ, IL-4, IL-5, IL-10, IL-13, TGF-β, and IL-17) were simultaneously determined using ELISA and further analyzed.ResultsIn the present study, no significant changes in Th1-type cytokines production were detected in early infection, both in primary and secondary infections, while the Th2-type response was strongly induced. A comparison of primary and secondary infection showed no significant difference in the cytokine secretion, which may indicate that the re-infection at 4 weeks after primary infection could not induce a robust adaptive immune response. The full extent of interaction between buffalo and F. gigantica in re-infection requires further study.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Secondary infection of Fasciola gigantica in buffaloes shows a similar pattern of serum cytokine secretion as in primary infection

et al. 2023

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“…They were confirmed free from fluke infection through indirect FgESP enzyme-linked immunosorbent assays (ELISA; Supplementary Table S2) and coprological examination (Zhang et al, 2006). In week 0, the infection group was given a gelatine capsule containing 250 viable F. gigantica metacercariae, while the non-infection group were mock-inoculated with 0.85% sodium chloride solution without metacercariae, the mean numbers of flukes recovered were 55.5 ± 14.1 (22.2 ± 5.6 of infection dose) in infection group (Wang et al, 2022b). Whole blood was collected from the non-infection (3, 10, and 16 wpi) and infection (0, 1, 3, 6, 8, 10, 13, and 16 wpi) groups for serum preparation and stored at −80°C until needed.…”

Section: Preparation Of Buffalo Serum Representing Different Infectio...mentioning

confidence: 99%

Proteomic analysis of Fasciola gigantica excretory and secretory products (FgESPs) co-immunoprecipitated using a time course of infected buffalo sera

et al. 2022

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IntroductionWidespread Fasciola gigantica infection in buffaloes has caused great economic losses in buffalo farming. Studies on F. gigantica excretory and secretory products (FgESP) have highlighted their importance in F. gigantica parasitism and their potential in vaccine development. Identifying FgESP components involved in F. gigantica-buffalo interactions during different periods is important for developing effective strategies against fasciolosis.MethodsBuffaloes were assigned to non-infection (n = 3, as control group) and infection (n = 3) groups. The infection group was orally administrated 250 metacercariae. Sera were collected at 3, 10, and 16 weeks post-infection (wpi) for the non-infection group and at 0 (pre-infection), 1, 3, 6, 8, 10, 13, and 16 wpi for the infection group. FgESP components interacting with sera from the non-infection and infection groups assay were pulled down by co-IP and identified using LC–MS/MS. Interacting FgESP components in infection group were subjected to Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway and gene ontology (GO) functional annotation to infer their potential functions.Results and discussionProteins of FgESP components identified in the non-infection group at 3, 10, and 16 wpi accounted for 80.5%, 84.3%, and 82.1% of all proteins identified in these three time points, respectively, indicating surroundings did not affect buffalo immune response during maintenance. Four hundred and ninety proteins were identified in the infection group, of which 87 were consistently identified at 7 time points. Following GO analysis showed that most of these 87 proteins were in biological processes, while KEGG analysis showed they mainly functioned in metabolism and cellular processing, some of which were thought to functions throughout the infection process. The numbers of specific interactors identified for each week were 1 (n = 12), 3 (n = 5), 6 (n = 8), 8 (n = 15), 10 (n = 23), 13 (n = 22), and 16 (n = 14) wpi, some of which were thought to functions in specific infection process. This study screened the antigenic targets in FgESP during a dense time course over a long period. These findings may enhance the understanding of molecular F. gigantica-buffalo interactions and help identify new potential vaccine and drug target candidates.

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Qualitative analysis of Fasciola gigantica excretory and secretory products coimmunoprecipitated with buffalo secondary infection sera shows dissimilar components from primary infection sera

Zheng,

Kong,

Jiang

et al. 2024

Acta Tropica

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Effect of primary and secondary Fasciola gigantica infection on specific IgG responses, hepatic enzyme levels and weight gain in buffaloes

Cited by 4 publications

References 28 publications

Secondary infection of Fasciola gigantica in buffaloes shows a similar pattern of serum cytokine secretion as in primary infection

Secondary infection of Fasciola gigantica in buffaloes shows a similar pattern of serum cytokine secretion as in primary infection

Proteomic analysis of Fasciola gigantica excretory and secretory products (FgESPs) co-immunoprecipitated using a time course of infected buffalo sera

Qualitative analysis of Fasciola gigantica excretory and secretory products coimmunoprecipitated with buffalo secondary infection sera shows dissimilar components from primary infection sera

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