Effect of Probiotic Bacillus megaterium PTB 1.4 on the Population of Intestinal Microflora, Digestive Enzyme Activity and the Growth of Catfish ( Clarias sp.)

Afrilasari, Wahyu; Widanarni, Widanarni; Meryandini, Anja

doi:10.1016/j.hjb.2016.12.005

Cited by 54 publications

(45 citation statements)

References 20 publications

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“…Given probiotics with the right dose helps postlarva tiger shrimp feed optimally utilize as needed nutrients in the body [9]. Probiotics in raising tiger shrimp have many benefits, especially for absorbing nutrients from feed [10].…”

Section: Growth Of Tiger Shrimpmentioning

confidence: 99%

Effect of Yeast and Lactic Acid Bacteria Probiotic on The Growth of Tiger Shrimp (Penaeus monodon), Microbiology and Water Quality

et al. 2020

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Yeast and lactic acid bacteria are often used as probiotics. This study aimed to determine the effect of giving yeast and LAB probiotic using fruit waste as a microbial medium on the productivity of tiger shrimp postlarvae (Panaeus monodon). As many as 120 tiger shrimp post-larvae were acclimatized for 2 days. The tiger shrimps post-larvae were divided into 4 treatments; a control group tiger shrimp post larvae without given yeast and LAB probiotic (P0); treatment 1 (P1); treatment 2 (P2) and treatment 3 (P3) in sequence shrimp post larvae given 25 ml; 50 ml; and 75 ml yeast and LAB probiotic/15 L of water. Yeast and LAB probiotic was given in shrimps post-larvae live media for 3 weeks(once per week). Data were analyzed using analysis of variance (ANOVA) following the Duncan test. The results of this study showed that yeast and LAB probiotic at a dose 25ml/15 L of water can increase the growth of tiger shrimp post-larvae, reduce the amount of Vibrio sp. and maintain the quality of tiger shrimp post larvae environment.

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Section: Growth Of Tiger Shrimpmentioning

confidence: 99%

Effect of Yeast and Lactic Acid Bacteria Probiotic on The Growth of Tiger Shrimp (Penaeus monodon), Microbiology and Water Quality

et al. 2020

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“…From this literature review, we can highlight the novel investigations carried out in Oncorhynchus mykiss, Seriola dumerili, and Sparus aurata in which it is shown that the probiotics of the genera Bacillus, Lactobacillus, and Enterococcus have the capacity to influence the immune system. In this regard, the most widely used probiotics in aquaculture are Bacillus and Lactobacillus because they have better yield in feed conversion, growth rate, weight gain [22,42,43], increase in digestive activity [44], increase in the growth performance of the fish [45], immunostimulant [46] and antagonistic activity against Vibrios [47]. In addition, according to the literature, it is common to find probiotics of the genus Pseudoalteromonas sp.…”

Section: Application Of Probiotics In Aquaculture Of Fishmentioning

confidence: 99%

Probiotic Bacteria as an Healthy Alternative for Fish Aquaculture

Sayes¹,

Leyton²,

Riquelme³

2018

Antibiotic Use in Animals

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One of the problems of the aquaculture industry is the presence of pathogenic microorganisms whose proliferation is enhanced when the healthy quality of the culture systems do not meet comply with physical-chemical-biological parameters. In order to improve these problems, less aggressive alternatives to the environment have been sought. This is why probiotic bacteria are proposed as an alternative to the same systems where they will be applied, since they generate greater interest in not presenting a threat to the ecosystem, favor survival, improve the immune system of organisms and have antibacterial properties against pathogenic bacteria. This chapter reviews current research related to the search for marine probiotics for application in the aquaculture industry. Additionally, we deliver results from our work related to the research and application of probiotics. The reported studies demonstrate the positive effects of marine bacteria for their aquaculture application. The evidences found in our work allow us to conclude that larval survival is favored by the application of probiotics in the use of vectors such as rotifers, artemia and biofilms. However, depending on the species of interest, it is necessary to study the market for the biotechnological application of probiotics, to evaluate the feasibility of its production on a larger scale and its commercial feasibility.

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“…The preparation for gnotobiotic catfish was done by adding different types of antibiotics into the water, they were 250 mg/L of ampicillin, 125 mg/L of rifampicin, and 250 mg/L of chloramphenicol for the reduction of bacteria in the outside part of the fish body. After that, the fish was being fast for 24 hours, until then they were given feed that containing 100 µg/mL of rifampicin for the reduction of the bacteria in the digestive tract for 4-5 days (Afrilasari, 2017). The utilization of rifampicin is based on the sensitivity result of catfish's intestinal bacteria that showed the intestinal bacteria of catfish tend to be sensitive to rifampicin more than other antibiotics.…”

Section: The Experimental Preparation Of Normal Catfish and Gnotobiotmentioning

confidence: 99%

“…The density of probiotics P. pentosaceus E2211 that added into the feed for viability test is 10 8 and 10 10 CFU/ml (Table 3). Afrilasari et al (2017) reported that the results of viability test of probiotic Bacillus megaterium PTB 1.4 (the density of 10 8 CFU/ mL) that added into feed is able to survive with a cell density of 10 6 CFU/mL and significantly affect the total intestinal bacteria of catfish, while Zare et al (2015) reported that the addition of probiotics Pediococcus acidilactici with density of 10 10 CFU/ml also give the significant effects towards the total number of LAB in intestine.…”

Section: The Viability Of Probiotic Bacteria In Fish Feedmentioning

confidence: 99%

Selection of lactic acid bacteria as a probiotic and evaluated its performance on gnotobiotic catfish Clarias sp.

Turnip

Widanarni

Meryandini

2018

Jurnal Akuakultur Indonesia

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ABSTRACT This study aimed to select lactic acid bacteria (LAB) as a potential probiotic that producing anti‒microbial compounds in order to treat motile aeromonads septicemia diseases caused by Aeromonas hydrophila on catfish Clarias sp. and evaluated its performance on gnotobiotic catfish. The in vitro assay was done to select several LAB isolates based on antagonistic activity against pathogenic bacteria. The selected isolate was tested in vivo to observe their ability to improve growth performances of catfish. The study was conducted with five treatments consists of K‒ (normal catfish without addition probiotic, without challenge test), K+ (normal catfish without addition of probiotic, with challenge test), Np (normal catfish with addition of probiotic and challenge test), G (gnoto catfish without addition of probiotic, with challenge test), and Gp (gnoto catfish with addition of probiotic and challenge test). The results showed that the addition of Pediococcus pentosaceus E2211 as selected probiotic could increase survival rate, specific growth rate, and immune response towards infection of A. hydrophila. The best survival rate after challenge test was obtained in Np and Gp treatments (88.46%), followed by G treatment (65.38%), while the K+ was only 53.84%. The conclusion of this study was P. pentosaceus E2211 potentially used as a probiotic candidate for normal and gnotobiotic catfish. The presence of normal microflora with P. pentosaceus E2211 in Np treatment showed the best probiotic performance with daily growth rate 3.28%, feed conversion ratio 1.79, and total intestinal bacteria reached 108 CFU/mL significantly different from other treatments (P<0.05).Keywords: Aeromonas hydrophila, catfish, LAB, probiotic, screening ABSTRAKTujuan penelitian ini adalah menyeleksi bakteri asam laktat (BAL) sebagai probiotik potensial penghasil senyawa antimikrob guna menanggulangi penyakit motile aeromonad septicemia akibat Aeromonas hydrophila pada ikan lele Clarias sp. dan evaluasi kinerjanya pada ikan lele gnotobiotik. Pengujian in vitro dilakukan untuk menyeleksi beberapa isolat BAL sebagai kandidat probiotik berdasarkan aktivitas antagonis terhadap bakteri patogen. Isolat terpilih kemudian diuji in vivo untuk mengetahui kemampuannya dalam meningkatkan performa tumbuh ikan lele. Penelitian ini menggunakan rancangan acak lengkap dengan lima perlakuan, yaitu: K‒ (lele normal tanpa probiotik dan tanpa tanpa diuji tantang), K+ (lele normal tanpa probiotik dan diuji tantang), Np (lele normal diberi probiotik dan diuji tantang), G (lele gnoto tanpa probiotik dan diuji tantang), dan Gp (lele gnoto diberi probiotik dan diuji tantang). Hasil penelitian menunjukkan pemberian probiotik terpilih BAL Pediococcus pentosaceus E2211 mampu meningkatkan sintasan, laju pertumbuhan, dan respons imun ikan lele terhadap infeksi A. hydrophila. Sintasan terbaik pascauji tantang diperoleh pada perlakuan Np dan Gp yaitu sebesar 88,46%, diikuti perlakuan G sebesar 65,38%, sementara pada K+ hanya mencapai 53,84%. Kesimpulan dari penelitian ini ialah isolat BAL terpilih P. pentosaceus E2211 berpotensi sebagai kandidat probiotik untuk ikan lele normal dan lele gnotobiotik Clarias sp. Keberadaan mikroflora normal yang berasosiasi dengan P. pentosaceus E2211 pada perlakuan Np menunjukkan kinerja probiotik terbaik dengan nilai laju pertubuhan harian 3,28%, rasio konversi pakan 1,79 dan total bakteri usus mencapai 108 CFU/mL yang berbeda signifikan dibanding perlakuan lainnya (P<0,05).Kata kunci: A. hydrophila, BAL, ikan lele, probiotik, seleksi

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Effect of Probiotic Bacillus megaterium PTB 1.4 on the Population of Intestinal Microflora, Digestive Enzyme Activity and the Growth of Catfish ( Clarias sp.)

Cited by 54 publications

References 20 publications

Effect of Yeast and Lactic Acid Bacteria Probiotic on The Growth of Tiger Shrimp (Penaeus monodon), Microbiology and Water Quality

Effect of Yeast and Lactic Acid Bacteria Probiotic on The Growth of Tiger Shrimp (Penaeus monodon), Microbiology and Water Quality

Probiotic Bacteria as an Healthy Alternative for Fish Aquaculture

Selection of lactic acid bacteria as a probiotic and evaluated its performance on gnotobiotic catfish Clarias sp.

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