Effect of prolactin on freshwater survival and on plasma osmotic pressure of hypophysectomized Tilapia mossambica

Dharmamba, Muthusamy; Handin, Robert I.; Nandi, Jean; Bern, Howard A.

doi:10.1016/0016-6480(67)90064-0

Cited by 62 publications

(13 citation statements)

References 18 publications

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“…PRL is essential for maintaining osmoregulatory homeostasis by euryhaline teleosts in FW (Pickford and Phillips, 1959;Dharmamba et al, 1967). Prolactin acts by reducing water permeability and increasing ion uptake, at least in part by upregulating NCC mRNA expression in the gills (Breves et al, 2010b).…”

Section: Discussionmentioning

confidence: 99%

“…The hypophyseal hormone, prolactin (PRL), plays an essential role in FW osmoregulation in euryhaline teleosts (Pickford and Phillips, 1959;Dharmamba et al, 1967;Bern, 1983;Manzon, 2002). Consistent with its activity in FW, PRL release in Mozambique tilapia increases in response to physiologically relevant reductions in extracellular osmolality both in vivo and in vitro (Grau et al, 1981;Yada et al, 1994;Seale et al, 2002Seale et al, , 2006bSeale et al, , 2012c.…”

Section: Introductionmentioning

confidence: 97%

“…Extensive work has provided an understanding of how tilapia in FW and SW adapt to acute osmotic challenges (Dharmamba et al, 1967(Dharmamba et al, , 1973Ayson et al, 1993;Yada et al, 1994;Heijden et al, 1997;Sakamoto et al, 1997;Shepherd et al, 1999;Seale et al, 2002Seale et al, , 2006bSeale et al, , 2012bInokuchi et al, 2008Inokuchi et al, , 2009Ouattara et al, 2009;Breves et al, 2010bBreves et al, ,c,d, 2011Velan et al, 2011). While the effects of a salinity change on PRL cells and branchial mediators of ion transport are well documented in tilapia subjected to one-way transfers between two salinities, less is known about fish subjected to cyclic variations in salinity.…”

Section: Introductionmentioning

confidence: 99%

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The effects of acute salinity challenges on osmoregulation in Mozambique tilapia reared in a tidally changing salinity

Moorman

Lerner

Grau

et al. 2015

Journal of Experimental Biology

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This study characterizes the differences in osmoregulatory capacity among Mozambique tilapia, Oreochromis mossambicus, reared in freshwater (FW), in seawater (SW) or under tidally driven changes in salinity. This was addressed through the use of an abrupt exposure to a change in salinity. We measured changes in: (1) plasma osmolality and prolactin (PRL) levels; (2) pituitary expression of prolactin (PRL) and its receptors, PRLR1 and PRLR2; (3) branchial expression of PRLR1, PRLR2, Na− co-transporter (NKCC), α1a and α1b isoforms of Na + /K + -ATPase (NKA), cystic fibrosis transmembrane conductance regulator (CFTR), aquaporin 3 (AQP3) and Na + /H + exchanger 3 (NHE3). Mozambique tilapia reared in a tidal environment successfully adapted to SW while fish reared in FW did not survive a transfer to SW beyond the 6 h sampling. With the exception of CFTR, the change in the expression of ion pumps, transporters and channels was more gradual in fish transferred from tidally changing salinities to SW than in fish transferred from FW to SW. Upon transfer to SW, the increase in CFTR expression was more robust in tidal fish than in FW fish. Tidal and SW fish successfully adapted when transferred to FW. These results suggest that Mozambique tilapia reared in a tidally changing salinity, a condition that more closely represents their natural history, gain an adaptive advantage compared with fish reared in FW when facing a hyperosmotic challenge.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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The effects of acute salinity challenges on osmoregulation in Mozambique tilapia reared in a tidally changing salinity

Moorman

Lerner

Grau

et al. 2015

Journal of Experimental Biology

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“…In fish, PRL is considered to be the major fresh water adapting hormone, given its ability to regulate the hydromineral balance in the euryhaline teleosts (Hirano 1986). In tilapia, PRL has been shown to be essential for the survival in fresh water of hypophysectomized fish (Dharmamba et al 1967). Among other osmoregulatory actions, PRL is known to reduce Na + efflux and water permeability at the level of the gill (Dharmamba & Maetz 1972, Dharmamba et al 1976, Wendelaar Bonga & Van der Meij 1981.…”

Section: Introductionmentioning

confidence: 99%

Expression of the prolactin receptor (tiPRL-R) gene in tilapia Oreochromis niloticus: tissue distribution and cellular localization in osmoregulatory organs

Sandra¹,

Rouzic²,

Cauty³

et al. 2000

Journal of Molecular Endocrinology

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The expression of the prolactin receptor (PRL-R) gene has been investigated in various tissues of tilapia (Oreochromis niloticus) reared in fresh or brackish water. Using a cDNA probe spanning the extracellular domain of the tilapia PRL-R and Northern blot analysis, the presence of tilapia PRL-R mRNA has been confirmed in the osmoregulatory organs and has been detected in other tissues, including the skin, the brain, the reproductive organs, and the two major hematopoietic organs (spleen and head kidney), as well as circulating lymphocytes. These findings suggest a conservation of the physiological processes regulated by prolactin throughout the vertebrates, including immunity and central nervous activity. A non-radioactive in situ hybridization procedure has allowed us to detect the expression of the tilapia PRL-R in the branchial chloride cells and the intestinal mucosal layer of fresh water animals, confirming the direct control exerted by prolactin on the water and ionic exchanges in tilapia. In all the tissues examined one unique PRL-R transcript has been detected with a similar size (3·2 kb) whatever the salinity conditions. Thus, the transcriptional expression of the tilapia PRL-R strongly differs from the complex RNA pattern reported for the higher vertebrates PRL-R and provides an additional argument for the existence of a single PRL-R for both prolactin isoforms in this fish species.

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“…Although an agreement is far from being reached [Adler et al, 1975;Berl et al, 1976;Epstein et al, 1977;Dearlove, 1981;Bliss and Lote, 1982], several reports have proposed an association between PRL and osmoregula tion, the hormone being osmotically regu lated [Buckman and Peake, 1973;Buckman et al, 1973;Relkin, 1974;Andersen et al, 1982] , or acting on osmotic regulation [Dharmamba et al, 1967;Josimovich et al, 1977;Ensor, 1978;Leontic et al, 1979;Gopalakrishnan et al, 1980;Mor ley et al, 1981]. Since loss of endogenous heat generated by contracting muscles can lead to marked blood fluid shifts [Senay, 1979], we postu lated that prolactinemic changes observed during exercise could be associated with vari ations in core temperature and/or in blood osmolality.…”

Section: Introductionmentioning

confidence: 99%

Exercise-Induced Blood Prolactin Variations in Trained Adult Males: A Thermic Stress More than an Osmotic Stress

Brisson¹,

Audet

Ledoux

et al. 1986

Horm Res

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Blood prolactin (PRL) variations have been linked to temperature and osmotic changes in several species. The latter factors are here explored to better understand blood PRL responses frequently induced during physical exercise. Since body heat generated by exercise can lead to marked body fluid shifts, it was postulated that PRL changes observed during exercise could be associated with variations in body temperature and/or blood osmolality (OSM). A wide range (38.5–40.5°C) of rectal temperatures (Tr; used here to appreciate core temperatures) were theoretically selected and randomly assigned as targets to male runners. Measured by thermistor probe, target Tr were obtained by a combination of factors: (a) ↑ heat production by treadmill running, and (b) ⇓ heat losses by appropriate clothing (⇓ evaporation) in warmed (⇓ radiation) and hypo ventilated (⇓ convection) laboratory conditions. For each subject, target Tr was attained not prior to 30 min after initiation of running, and had to be maintained for at least 10 min, for a mean ( ± SD) running time of 52.6 ± 10.0 min. In a first protocol, hypohydration was provoked in 26 runners (23.9 ± 4.7 years) by total restriction of water intake. In a second protocol (10 different runners: 22.3 ± 3.3 years), euhydration was maintained by water intake (20 ml/kg body weight). Venous blood was sampled at rest before and immediately after the run. PRL was assayed by RIA; OSM was measured by freezing point depression; sodium was analyzed by flame photometry. At rest, before the heat-producing exercise, mean PRL values were 9.4 ± 3.4 ng/ml for both eu/hypohydrated groups. In the hypohydrated runners, exercise-induced hyperthermia was significantly (r = 0.82; p < 0.0005) associated with blood PRL responses. Moreover, these changes in Tr were also significantly (r = 0.54; p < 0.0025) related to changes in OSM, the latter variations being mostly explained (78 %) by the accompanying hypernatremia. In the euhydrated group of runners, the hyperthermic exercise failed to induce significant changes in OSM (r = 0.22; p > 0.15) and, as expected, variations in blood sodium levels were also not significant under these conditions. However, hyperthermic running in these iso-osmolar conditions did not prevent blood PRL levels from rising (r = 0.77; p < 0.0005). It was thus concluded that, in male trained runners, exercise-induced blood PRL responses could be derived more from thermic than from osmolar stresses.

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Effect of prolactin on freshwater survival and on plasma osmotic pressure of hypophysectomized Tilapia mossambica

Cited by 62 publications

References 18 publications

The effects of acute salinity challenges on osmoregulation in Mozambique tilapia reared in a tidally changing salinity

The effects of acute salinity challenges on osmoregulation in Mozambique tilapia reared in a tidally changing salinity

Expression of the prolactin receptor (tiPRL-R) gene in tilapia Oreochromis niloticus: tissue distribution and cellular localization in osmoregulatory organs

Exercise-Induced Blood Prolactin Variations in Trained Adult Males: A Thermic Stress More than an Osmotic Stress

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