Effect of prolonged aflatoxin B₁ administration on blood serum oestradiol-17β and progesterone concentrations of goats during the anoestrus period --

Abstract

ABSTRACT:The effect of prolonged aflatoxin B 1 (AFB 1 ) administration on blood serum oestradiol-17β and progesterone concentrations in goats during the anoestrus period was investigated. Thirty Greek indigenous goats were used; 10 goats received, per os, 50 μg (treated group T50) and 10 goats received 100 μg (treated group T100) AFB 1 /day/head, respectively, for 31 days, while 10 goats served as controls (C). Blood samples were collected from each goat twice a week to determine serum oestradiol-17β and progesterone concentrations by radioimmunoassay. Multiple linear regression analysis revealed a significant (P < 0.05) positive dependence of blood serum oestradiol-17β concentration over group (C = 0, T50 = 50, T100 = 100), in a dosedependent manner, and a significant (P < 0.05) negative dependence over time (0-31 days, from the onset to the end of AFB 1 administration). Linear regression analysis revealed a significant (P < 0.05) positive dependence of blood serum progesterone concentration over group (C = 0, T50 = 50, T100 = 100), in a dose-dependent manner. Pearson's correlation coefficient revealed a significant (P < 0.05) positive correlation between blood serum oestradiol-17β and progesterone concentration, but only in the goats of the control group. In conclusion, the prolonged administration of 100 or even of 50 μg AFB 1 /day/head during the anoestrus period, increased blood serum oestradiol-17β and progesterone concentrations of goats, in a dose-dependent manner, and in the treated groups disturbed the positive correlation between oestradiol-17β and progesterone concentration shown in the goats of the control group.

Effect of aflatoxin B1 on blood serum oestradiol-17² and progesterone concentrations during the luteal phase and the synchronized oestrus of goats

“…Furthermore, the studies that have already been performed, mainly in laboratory animals, use quite higher concentrations and/or shorter periods of AFB 1 administration. In a previous study ( Kourousekos et al ., 2015 ) the prolonged administration of 100 or even of 50 μg AFB 1 /day/head increased blood serum oestradiol-17β and progesterone concentrations in anoestrous goats, in a dose dependent manner. Thus, the present study was conducted in order to investigate the possible effects by AFB 1 administration on blood serum oestradiol-17β and progesterone concentrations, during the luteal phase and the synchronized oestrus and consequently on ovarian activity and oestrus cycle of goats being in oestrus period.…”

“…The authors proposed either a direct effect of AFB 1 on testes secreting cells or on the hypothalamus-hypophysis-testes axis. Furthermore during the anoestrus period of the goats the prolonged per os administration of 100 or even of 50 μg AFB 1 /day/head increased blood serum oestradiol-17β and progesterone concentrations, in a dose dependent manner ( Kourousekos et al ., 2015 ).…”

“…The experiment lasted approximately 1.5 month, during which the goats of T50 or T100 groups received 50 or 100 μg AFB 1 /day/head, respectively [doses by which AFM 1 is excreted into the milk, exceeding the maximum permissible level (50 ng/L) set by the European Union (Kourousekos et al, 2012)]. Ten mg of pure AFB 1 (AFB 1 from Aspergillus flavus, A 6636-10 MG, SIGMA, Sigma Chemical Co, St. Louis, MO, USA) were dissolved in 100 or 200 mL methanol and 1 mL of this dilution was received per os by each goat of T50 or T100 treated groups, respectively.…”

Caffeic acid mitigates aflatoxinB1‐mediated toxicity in the male rat reproductive system by modulating inflammatory and apoptotic responses, testicular function, and the redox‐regulatory systems

“…Typically, decreases in steroidogenesis is a feature AFB 1 ‐mediated toxicity to the reproductive system including spermatogenesis (Supriya et al, 2014), quantity, quality, and morphology of sperm due to aflatoxicosis (Kourousekos & Theodosiadou, 2018). It has also been shown to negatively influence the concentrations of hormones, causing hormonal disruptions and toxicity to the cells (Ortatatli et al, 2002) and impair experimental animals’ sexual development and behavior (Shuaib et al, 2010; Kourousekos et al, 2016; Marijani et al, 2019). The toxicity of AFB 1 is not limited to the reproductive system.…”