Effect of prolonged ouabain treatment on Na, K, Cl and Ca concentration and fluxes in cultured human cells

Lamb, J. F.; McCall, David

doi:10.1113/jphysiol.1972.sp009959

Cited by 84 publications

(30 citation statements)

References 22 publications

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“…The data of Table 2 do not directly support hypotheses invoking either constancy of transmembrane ion gradients (Thomas, 1972) (Lamb & McCall, 1972 Lewis (1952), Deffner (1961), Sorenson (1973) and McBride et al (1974). Again the estimates are quite variable, both within one set of analyses, and in one nerve analysed by different investigators (e.g.…”

Section: Protein and Lipidmentioning

confidence: 57%

Axoplasm chemical composition in Myxicola and solubility properties of its structural proteins.

Gilbert¹

1975

The Journal of Physiology

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SUMMARY1. The chemical composition of axoplasm extracted from the giant axon of Myxicola infundibulum has been analysed, and some of the factors which disperse its gel structure have been identified.2. The axoplasm contains about 3-6% protein, and 0-12% lipid. It is isosmotic with sea water and has a pH near 7 0.3. Inorganic ions in extracted axoplasm include: Na+, 13 m-mole/kg wet wt.; K+, 280; 24; Ca2 , 0 3; Mg2+, 3. 4. Free organic ions in axoplasm include: gly, 180 m-mole/kg wet wt.; cysteic acid, 120; asp, 75; glu, 10; ala, 7; tau, 5; thr, 2; gln and ser, trace; homarine, 63; isethionate, 0.5. The gel structure is dispersed by solutions containing 1-10 mM-Ca2+, because this ion activates an endogenous protease. The gel can also be dispersed without proteolysis by solutions containing 0 5 M-KCl, or 0 5 M guanidine hydrochloride, or 0 5 M urea, all of which break down neurofilaments.6. It is argued that many aspects of the composition and dispersal properties of Myxicola axoplasm are similar to those in other axons.

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Section: Protein and Lipidmentioning

confidence: 57%

Axoplasm chemical composition in Myxicola and solubility properties of its structural proteins.

Gilbert¹

1975

The Journal of Physiology

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“…1, 1982 22 T. IKEHARA, T. SAKAI, H. MIYAMOTO, and K. KANIIKE and K+ concentrations (ANNER, 1980), and also the ratio was changed as a function of cell Na+ in squid axons (MULLINS and BRINLEY, 1969;BRINLEY and MULLINS, 1974). In cultured heart cells, the coupling ratio was calculated at 1.5 under physiological conditions, whereas it was increased by treatment with low concentrations of ouabain (LAMB and MCCALL, 1972). These results imply that the Na+/K+ coupling ratio can vary when cellular ionic conditions are modified.…”

Section: Discussionmentioning

confidence: 99%

Interrelation between membrane transport and the contents of alkali metal cations in HeLa cells.

et al. 1982

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“…The growth of HeLa cells cultured in a medium supplemented with human serum was inhibited as the extracellular K+ concentration decreased (43). Ouabain is reported to reduce the rate of cell growth or protein synthesis by lowering either the K+ influx or its intracellular content (13,20,22,27). Thus, protein synthesis and cell growth are regarded as dependent on the intracellular K+ concentration.…”

Section: Discussionmentioning

confidence: 99%

Effect of Rb+ Substituted for K+ on HeLa Cells: Cellular Content and Membrane Transport of Monovalent Cations, and Cell Growth

Miyamoto

Sakai

Ikehara

et al. 1978

Cell Struct. Funct.

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ABSTRACT. The usefulness of Rb+ was investigated as an analog of K+ in membrane transport and in supporting the growth of HeLa cells. On replacement of extracellular K+ by Rb+, intracellular Rb+ content increased linearly at 15 min. K+ content declined in contrast with Rb+, but Na+ content was unaffected. In Rb+-preloaded cells, Rb+ and K+ were exchanged after replacement of extracellular Rb+ with K. A kinetic analysis of intracellular Rb+ content showed that most K+ was exchangeable with Rb+, but a small part of K+ was not. Both Rb+ and K+ influxes were equally inhibited by ouabain or furosemide. Rb+ efflux was repressed by furosemide, whereas K+ efflux was not influenced. When 90 % of K+ was replaced by Rb+ in serum-free chemically defined medium, cell growth and protein synthesis were inhibited. After total replacement of extracellular K+ by Rb+, cell growth ceased and cellular protein content decreased, while a small part of intracellular K+ remained unexchanged. HeLa cells seemed to accumulate Rb+ in preference to K+.

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Effect of prolonged ouabain treatment on Na, K, Cl and Ca concentration and fluxes in cultured human cells

Abstract: [K], by prolonged treatment increases the total Na fluxes and decreases the total K fluxes but * Present address and address for reprints: The Physiology Department, Bute

Cited by 84 publications

References 22 publications

Axoplasm chemical composition in Myxicola and solubility properties of its structural proteins.

Axoplasm chemical composition in Myxicola and solubility properties of its structural proteins.

Interrelation between membrane transport and the contents of alkali metal cations in HeLa cells.

Effect of Rb+ Substituted for K+ on HeLa Cells: Cellular Content and Membrane Transport of Monovalent Cations, and Cell Growth

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