Effect of proton pump inhibitor on microbial community, function, and kinetics in anaerobic digestion with ammonia stress

Yu, Dawei; Zhang, Qingqing; Jaegher, Bram De; Liu, Jibao; Sui, Qianwen; Zheng, Xiang; Wei, Yuansong

doi:10.1016/j.biortech.2020.124118

Cited by 24 publications

(8 citation statements)

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“…There are several key enzymes involved in providing the proton pump that powers the ATP synthetase to catalyze the conversion of ADP to ATP, including NADH dehydrogenase, succinate dehydrogenase, cytochrome bd complex, and cytochrome c oxidase (Figure ), and the genes that encoded all these related enzymes basically had higher expressions with limited CaO 2 addition (Table S2). Specifically, NADH dehydrogenase plays a crucial role in maintaining a suitable NADH/NAD + ratio . The mitochondrial electron transport chain can utilize the reducing equivalents generated from carbohydrate metabolism to power the synthesis of ATP, and in this study, the promotion of the TCA cycle and the PPP indicated a higher NADH level, so the gene abundance for NADH dehydrogenase was improved to convert the generated NADH to NAD + ; otherwise, the cumulative NADH/NAD + ratio would impede related carbohydrate metabolism due to feedback inhibition and even damage the cell membrane owing to a potential difference overload .…”

Section: Resultsmentioning

confidence: 71%

“…Specifically, NADH dehydrogenase plays a crucial role in maintaining a suitable NADH/NAD + ratio. 60 The mitochondrial electron transport chain can utilize the reducing equivalents generated from carbohydrate metabolism to power the synthesis of ATP, 61 and in this study, the promotion of the TCA cycle and the PPP indicated a higher NADH level, so the gene abundance for NADH dehydrogenase was improved to convert the generated NADH to NAD + ; otherwise, the cumulative NADH/NAD + ratio would impede related carbohydrate metabolism due to feedback inhibition and even damage the cell membrane owing to a potential difference overload. 62 Succinate dehydrogenase is an important membrane-bound enzyme of the TCA cycle, and its improved expression might be associated with the promoted TCA cycle with limited CaO 2 addition.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Facilitating Digester Recovery from Acid Inhibition at High Organic Load Rates by Limited Calcium Peroxide Addition

Tian

et al. 2022

ACS Sustainable Chem. Eng.

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This study evaluated the performance of limited calcium peroxide (CaO2) addition in maintaining the system stability of anaerobic digestion using lignocellulose biomass as a substrate at high organic load rates. Results showed that an overloaded digester, which had been on the verge of system collapse, regained its stability after 20 mg/L CaO2 was periodically added. Especially, limited CaO2 addition significantly reduced the volatile fatty acid (VFA) concentration and restored pH levels, thus enhancing methane production from 24.6 ± 0.4 to 80.9 ± 0.7 mL/g·VS. By combining the reactor performance, carbon mass balance, cell vitality of the microbial community, and metagenomic analyses, this study found that limited CaO2 addition stimulated the aerobic conversion of the VFA precursor to CO2 for VFA level control by facultative bacteria, which triggered their higher energy metabolism. Additionally, after limited CaO2 addition, acetotrophic and hydrogenotrophic methanogeneses were both promoted.

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Section: Resultsmentioning

confidence: 71%

Section: ■ Results and Discussionmentioning

confidence: 99%

Facilitating Digester Recovery from Acid Inhibition at High Organic Load Rates by Limited Calcium Peroxide Addition

Tian

et al. 2022

ACS Sustainable Chem. Eng.

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“…Such variations might have stemmed from the wide range of ammonia concentrations found in PM (from 3–13 gN/L). ,,− , Higher ammonia concentrations can negatively impact microbial health by compromising cell membrane function, leading to decreased integrity, elevated permeability, depolarization, and diminished fluidity . Additionally, the energy expenditure for microbes to regulate intracellular pH through the potassium pump increases due to the diffusion of free ammonia. , Despite this, methanogens have shown an ability to withstand certain levels of ammonia toxicity, with thresholds identified at 4 and 7 gN/L for acetoclastic and hydrogenotrophic methanogenesis, respectively …”

Section: Resultsmentioning

confidence: 99%

“…128 Additionally, the energy expenditure for microbes to regulate intracellular pH through the potassium pump increases due to the diffusion of free ammonia. 129,130 Despite this, methanogens have shown an ability to withstand certain levels of ammonia toxicity, with thresholds identified at 4 and 7 gN/L for acetoclastic and hydrogenotrophic methanogenesis, respectively. 131 CM, which contributes nitrogen as protein (30−60%) and uric acid (40−70%), 132,133 demonstrated a consistent synergistic relationship in CM:LB AcoD (RSI = 1.70) across subgroups (ROM = 1.70 in both CM:LB (n = 14) and LB:CM (n = 2)) (Figure 1).…”

Section: Co-digestion Of Agricultural Wastes and Residuesmentioning

confidence: 99%

Understanding Anaerobic Co-digestion of Organic Wastes through Meta-Analysis

Chuenchart,

Surendra,

Khanal

2024

ACS EST Eng.

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Anaerobic co-digestion (AcoD) is becoming increasingly popular in the biogas industry for its numerous advantages over mono-digestion, including balanced nutrient profiles, enhanced process stability, synergistic methane production, and reduced greenhouse gas emissions. However, varying results across AcoD studies highlight the need for an extensive meta-analysis of a large data set to better understand these synergies. Here, we compared methane yields from 432 data sets, based on the ratio of means, from AcoD and mono-digestion. The relative synergistic index (RSI) revealed synergistic effects in AcoD of lignocellulosic biomass with animal manures, particularly pig (RSI = 1.91) and chicken manures (RSI = 1.71). Due to its rapid biodegradability, food waste AcoD also demonstrated synergistic effects with both animal manures (RSI = 1.28) and lignocellulosic biomass (RSI = 1.41). After regrouping data by high-carbon cosubstrates, interpretable machine learning models identified temperature, which accelerates the hydrolysis of lignocellulosic biomass and emulsification of fats, oils, and grease, as the key factor influencing methane yield. Furthermore, experimental validation using food waste and sewage sludge co-digestion confirmed the superiority of multivariable linear regression in predicting specific methane yield over other models in terms of simplicity and efficiency.

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“…Random forest (RF) analysis was used to test the most important microbial taxa across the intercropping systems at the two sampling times by the randomForest package v 4.7-1 (Liaw and Wiener, 2002 ). Establishing an RF classifier, which contains a multitude of decision trees based on the threshold abundance of the critical genus (Yu et al, 2021 ). To enhance the RF classification performance, the optimal three RF hyper-parameters were searched through the “train” function from the caret package v 6.0-91 (Kuhn et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Melon/cowpea intercropping pattern influenced the N and C soil cycling and the abundance of soil rare bacterial taxa

et al. 2022

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The high use of pesticides, herbicides, and unsustainable farming practices resulted in losses of soil quality. Sustainable farming practices such as intercropping could be a good alternative to traditional monocrop, especially using legumes such as cowpea (Vigna unguiculata L. Walp). In this study, different melon and cowpea intercropping patterns (melon mixed with cowpea in the same row (MC1); alternating one melon row and one cowpea row (MC2); alternating two melon rows and one cowpea row (MC3)) were assayed to study the intercropping effect on soil bacterial community through 16S rRNA region in a 3-year experiment. The results indicated that intercropping showed high content of total organic carbon, total nitrogen and ammonium, melon yield, and bacterial diversity as well as higher levels of beneficial soil microorganisms such a Pseudomonas, Aeromicrobium, Niastella, or Sphingomonas which can promote plant growth and plant defense against pathogens. Furthermore, intercropping showed a higher rare taxa diversity in two (MC1 and MC2) out of the three intercropping systems. In addition, N-cycling genes such as nirB, nosZ, and amoA were more abundant in MC1 and MC2 whereas the narG predicted gene was far more abundant in the intercropping systems than in the monocrop at the end of the 3-year experiment. This research fills a gap in knowledge about the importance of soil bacteria in an intercropping melon/cowpea pattern, showing the benefits to yield and soil quality with a decrease in N fertilization.

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Effect of proton pump inhibitor on microbial community, function, and kinetics in anaerobic digestion with ammonia stress

Cited by 24 publications

References 50 publications

Facilitating Digester Recovery from Acid Inhibition at High Organic Load Rates by Limited Calcium Peroxide Addition

Facilitating Digester Recovery from Acid Inhibition at High Organic Load Rates by Limited Calcium Peroxide Addition

Understanding Anaerobic Co-digestion of Organic Wastes through Meta-Analysis

Melon/cowpea intercropping pattern influenced the N and C soil cycling and the abundance of soil rare bacterial taxa

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