Effect of Pulsed High-Voltage Stimulation οn Pholiota Nameko Mushroom Yield

Abstract

A pulsed high voltage was applied to logs for mushroom cultivation to investigate an effect of pulse high voltage on mushroom growth. Inductive energy storage system was utilized to construct a pulsed power generator with compact size. Copper fuse of 0.03 mm diameter was used as an opening switch to interrupt large circuit current in short time. The voltage charged in primary energy storage capacitor was multiplied using secondary energy storage inductor. The output voltage of the pulsed power generator was 120 kV with 100 ns pulse width at 14 kV charging voltage of 0.22 µF primary energy storage capacitor and 15 µH secondary energy storage inductor. This pulsed high voltage was applied to 90 cm length logs inoculated Pholiota nameko fungus. The yield of Pholiota nameko mushrooms was successfully improved with the pulse voltage stimulation. The total weight of the cropped Pholiota nameko after the pulse voltage stimulation was 6.3 kg for fifteen logs and this value was 1.5 times larger than 4.3 kg of total weight without the stimulation. The number of logs with lower than 0.27 kg cropped Pholiota nameko weight which was an average value for control group was only one in fifteen, whereas four logs in control group showed almost zero weight of cropped Pholiota nameko.

High-Voltage Methods for Mushroom Fruit-Body Developments

“…The yield of L. edodes fruiting bodies was improved with high-voltage stimulation generated by the IES pulsed power generators. The effect of the pulsed voltage stimulation on some other types of mushroom such as P. microspora and L. decastes was also confirmed using an IES generator developed for the improvement of yield of mushroom production [8]. The harvested weight from log wood and/or sawdust substrates for mushroom cultivation was increased by applying a pulsed voltage as an electrical stimulation.…”

“…Grifola frondosa, P. microspora, F. velutipes, Hypsizygus marmoreus, P. ostreatus, P. eryngii, P. abalones and Agrocybe cylindracea [7,8]. The fruiting body yield in the electrically stimulated substrate was observed to be 130-180% greater than that without the electrical stimulation [7].…”

“…Frequencies of the fruit-body yield by impulse high-voltage stimulation to L. edodes of bed-logs without water submerged treatment[2].Physical Methods for Stimulation of Plant and Mushroom Development a) and (b) shows photograph and equivalent circuit of a compact pulsed power generator used for promotion of fruit-body formation in natural-log based mushroom cultivation[8]. An inductive energy storage (IES) system consists of a primary energy storage capacitor C, a closing switch GS, a secondary energy storage inductor L and an opening switch.…”

Fruit body formation of lentinula edodes by pulse electric field stimulations

“…The effectiveness of the short pulse voltage stimulation for some other kinds mushroom, such as Pholiota nameko (P. nameko), Lyophyllum decastes, was also confirmed using IES generator developed for improvement of the mushroom yield as electrical stimulation [7], [8]. In the result of these studies, the total cropped weight from logs and/or sawdust-based block for cultivation of the mushrooms increased by applying pulse voltage as an electrical stimulation.…”

A 21st century miniguide to sporophore morphogenesis and development in Agaricomycetes and their biotechnological potential

“…However, in an attempt to increase mushroom yields in log wood and/or sawdust-based substrates, other fruiting stimuli have been studied. A pulsed high-voltage stimulation technique has been applied as an electrical stimulation to accelerate fruiting and increase yield in woody substrates used for mushroom cultivation (Ohga et al, 2004;Takaki et al, 2009). Takaki et al (2014) applied electrical stimulation to a sawdustbased substrate of Lyophyllum decastes and natural logs, in which Lentinula edodes, Pholiota nameko, and Naematoloma sublateritium were grown, and observed fruit body induction and hydrophobin release from the vegetative hyphae (in Lentinula edodes) one day after the stimulation.…”