Effect of PVP on sperm capacitation status and embryonic development in cattle

Kato, Yoku; Nagao, Yoshikazu

doi:10.1016/j.theriogenology.2009.04.018

Cited by 34 publications

(31 citation statements)

References 36 publications

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“…Moreover, the concentration and volume used in the MI and MP were the same. Therefore, we speculate that the difference in the embryo production may have been due to the toxicity of the PVP present in the Percoll † (De Vos et al 1997;Strehler et al 1998;Kato and Nagao 2009). The use of a conventional Isolate † gradient was recently reported for bovine in vitro embryo production using sexed and unsexed sperm (Rodrı´guez Villamil et al 2012).…”

Section: Discussionmentioning

confidence: 94%

Isolate^® and Optiprep^® minigradients as alternatives for sperm selection in bovine in vitro embryo production

et al. 2014

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. 2014. Isolate † and Optiprep † minigradients as alternatives for sperm selection in bovine in vitro embryo production. Can. J. Anim. Sci. 94: 35Á42. The objective of this study was to evaluate alternatives in small volumes to conventional gradient of Percoll † on semen quality, in vitro embryo production, sex ratio and embryo survival after vitrification. Thawed semen was randomly allocated to one of four density gradient selection methods: (1) conventional Percoll † (P), (2) MiniPercoll (MP), (3) MiniIsolate (MI), and (4) MiniOptiprep (MO). Sperm kinetics and quality were evaluated. Use of P, MP and MI gradients did not affect sperm motility (P!0.05). However, there was a decrease in total and progressive sperm motility in MO (70.8 and 51.3% vs. 87.3 and 69.5% for P; 87.3 and 73% for MP; 92.3 and 78.8% for MI; PB0.05). The MO had lower membrane integrity compared with P, MP and MI (39.7 vs. 70.5, 72.3, 63.8%, respectively, PB0.05). The percentage of blastocysts produced was higher in MI than in MP and MO (21.1 vs. 16.1 and 16.9%, PB0.05) and similar to P (18.4%; P!0.05). Sex ratio and embryo survival after vitrification were similar among groups (P!0.05). Semen selected by Isolate and Optiprep gradient, at the concentrations and small volumes used, demonstrated similar characteristics and in vitro embryo production to conventional Percoll † gradient. † , en petits volumes, sur la qualite´de la semence, la production in vitro d'embryons, le rapport des sexes et la survie de l'embryon apre`s vitrification. Des semences de´congele´es ont e´te´attribue´es ale´atoirement a`une de quatre me´thodes de se´lection par gradient de densite´: (1) Percoll † traditionnel (P), (2) MiniPercoll (MP), (3) MiniIsolate (MI), et (4) MiniOptiprep (MO). La cine´tique et la qualite´des spermatozoı¨des ont e´te´e´value´es. L'utilisation des gradients P, MP et MI n'ont eu aucun effet sur la motilite´des spermatozoı¨des (P!0,05). Il y avait, cependant, une diminution des motilite´s totales et progressives dans le gradient MO (70,8 et 51,3 % c. 87,3 et 69,5 % pour P; 87,3 et 73 % pour MP; 92,3 et 78,8 % pour MI; PB0,05). Le gradient MO avait une inte´grite´de membrane infe´rieure aux gradients P, MP et MI (39,7 c. 70,5; 72,3; 63,8 % respectivement, PB0,05). Le pourcentage de blastocystes produits e´tait plus e´leve´apre`s gradient MI qu'apre`s les gradients MP et MO (21,1 c. 16,1 et 16,9 %, P B0,05) et similaire apre`s gradient P (18,4 %; P!0,05). Le rapport des sexes et la survie de l'embryon apre`s vitrification e´taient similaires parmi tous les groupes (P !0,05). Les semences choisies par gradients Isolate et Optiprep, aux concentrations et aux petits volumes utilise´s, de´montrent des caracte´ristiques et une production in vitro d'embryons similaires a`celles choisies par gradients Percoll † traditionnels.

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Section: Discussionmentioning

confidence: 94%

Isolate^® and Optiprep^® minigradients as alternatives for sperm selection in bovine in vitro embryo production

et al. 2014

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“…Previous reports suggest that treatment of sperm with certain reagents during an IVF procedure affects later development of the embryo (Way and Killian, 2006; Kato and Anagao, 2009; Gonçalves et al, 2010), and, additionally, that the pronuclear pattern of the zygote is closely related to blastocyst formation and quality (Ballaban et al, 2001). The higher percent of capacitated or acrosome-reacted sperm in the HP or HA exposed sperm could improve sperm penetration and pronuclear formation.…”

Section: Discussionmentioning

confidence: 99%

Effect of Glycosaminoglycans on <italic>In vitro</italic> Fertilizing Ability and <italic>In vitro</italic> Developmental Potential of Bovine Embryos

Kim¹,

Noh²,

Noh

et al. 2013

Asian Australas. J. Anim. Sci

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The glycosaminoglycans (GAGs) present in the female reproductive tract promote sperm capacitation. When bovine sperm were exposed to 10 μg/ml of one of four GAGs (Chondroitin sulfate, CS; Dermatan sulfate, DS; Hyaluronic acid, HA; Heparin, HP) for 5 h, the total motility (TM), straight-line velocity (VSL), and curvilinear velocity (VCL) were higher in the HP- or HA-treated sperm, relative to control and CS- or DS-treated sperm. HP and HA treatments increased the levels of capacitated and acrosome-reacted sperm over time, compared to other treatment groups (p<0.05). In addition, sperm exposed to HP or HA for 1 h before IVF exhibited significantly improved fertilizing ability, as assessed by 2 pronucleus (PN) formation and cleavage rates at d 2. Exposure to these GAGs also enhanced in vitro embryo development rates and embryo quality, and increased the ICM and total blastocyst cell numbers at d 8 after IVF (p<0.05). A real-time PCR analysis showed that the expression levels of pluripotency (Oct 4), cell growth (Glut 5), and anti-apoptosis (Bax inhibitor) genes were significantly higher in embryos derived from HA- or HP-treated sperm than in control or other treatment groups, while pro-apoptotic gene expression (caspase-3) was significantly lower in all GAG treatment groups (p<0.05). These results demonstrated that exposure of bovine sperm to HP or HA positively correlates with in vitro fertilizing ability, in vitro embryo developmental potential, and embryonic gene expression.

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“…This makes sperm DNA normally resistant to any genotoxic insults. Although PVP has been regarded as a chemically inert material, there are several reports confirming its adverse effects both in vitro and in vivo (2)(3)(4). It has been suggested that the nuclear deterioration in PVP-exposed cells possibly results from the general breakdown of the sperm membrane and subsequent necrotic processes (15).…”

Section: Discussionmentioning

confidence: 99%

“…Capturing and immobilizing a single sperm under a microscope is required for ICSI, and immobilization is facilitated by introducing the sperm into a viscous medium to reduce the sperm's motility. Polyvinylpyrrolidone (PVP) is being used successfully in human ICSI although there are concerns about its potential adverse effect on gametes and embryos (2)(3)(4). It has been shown that some amount of PVP is also being injected along with the sperm; the PVP then remains inside the oocyte for a prolonged period, as it cannot be digested by the lysosomal enzymes (2).…”

mentioning

confidence: 99%

Frozen-thawed spermatozoa from oligozoospermic ejaculates are susceptible to in situ DNA fragmentation in polyvinylpyrrolidone-based sperm-immobilization medium

Salian

Kalthur

Uppangala

et al. 2012

Fertility and Sterility

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Effect of PVP on sperm capacitation status and embryonic development in cattle

Cited by 34 publications

References 36 publications

Isolate^® and Optiprep^® minigradients as alternatives for sperm selection in bovine in vitro embryo production

Isolate^® and Optiprep^® minigradients as alternatives for sperm selection in bovine in vitro embryo production

Effect of Glycosaminoglycans on <italic>In vitro</italic> Fertilizing Ability and <italic>In vitro</italic> Developmental Potential of Bovine Embryos

Frozen-thawed spermatozoa from oligozoospermic ejaculates are susceptible to in situ DNA fragmentation in polyvinylpyrrolidone-based sperm-immobilization medium

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Effect of PVP on sperm capacitation status and embryonic development in cattle

Cited by 34 publications

References 36 publications

Isolate® and Optiprep® minigradients as alternatives for sperm selection in bovine in vitro embryo production

Isolate® and Optiprep® minigradients as alternatives for sperm selection in bovine in vitro embryo production

Effect of Glycosaminoglycans on &lt;italic&gt;In vitro&lt;/italic&gt; Fertilizing Ability and &lt;italic&gt;In vitro&lt;/italic&gt; Developmental Potential of Bovine Embryos

Frozen-thawed spermatozoa from oligozoospermic ejaculates are susceptible to in situ DNA fragmentation in polyvinylpyrrolidone-based sperm-immobilization medium

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Isolate^® and Optiprep^® minigradients as alternatives for sperm selection in bovine in vitro embryo production

Isolate^® and Optiprep^® minigradients as alternatives for sperm selection in bovine in vitro embryo production

Effect of Glycosaminoglycans on <italic>In vitro</italic> Fertilizing Ability and <italic>In vitro</italic> Developmental Potential of Bovine Embryos