Effect of quercetine and glutathione on the level of superoxide dismutase, catalase, malonyldialdehyde, blood pressure and neonatal outcome in a rat model of pre-eclampsia induced by NG-nitro-l-arginine-methyl ester

Resende

Hypertension in Pregnancy

et al. 2007

The present study demonstrated a protective effect of GSE in experimental preeclampsia since the deleterious effect induced by L-NAME that is, increased in stillbirth, hypertension and insulin resistance were significantly reduced by oral treatment with the extract. Probably an endothelium-dependent vasodilator effect and an antioxidant action play an important role on the effects of GSE in experimental preeclampsia.

Section: Discussionsupporting

confidence: 95%

Protective Action of a Hydroalcoholic Extract of a Vinifera Grape Skin on Experimental Preeclampsia in Rats

Resende

Hypertension in Pregnancy

et al. 2007

“…Further in the present study L-NAME administration showed lower pup weights suggesting that intrauterine growth retardation may a consequence of severe preeclampsia. Our findings are in line with other studies reporting lower fetal weights in L-NAME treated dams [65] – [67] . In contrast other studies report higher fetal weights [68] or no change in fetal weights [69] .…”

Section: Discussionsupporting

confidence: 93%

A Combined Supplementation of Omega-3 Fatty Acids and Micronutrients (Folic Acid, Vitamin B12) Reduces Oxidative Stress Markers in a Rat Model of Pregnancy Induced Hypertension

2014

ObjectivesOur earlier studies have highlighted that an altered one carbon metabolism (vitamin B12, folic acid, and docosahexaenoic acid) is associated with preeclampsia. Preeclampsia is also known to be associated with oxidative stress and inflammation. The current study examines whether maternal folic acid, vitamin B12 and omega-3 fatty acid supplementation given either individually or in combination can ameliorate the oxidative stress markers in a rat model of pregnancy induced hypertension (PIH).Materials and MethodsPregnant Wistar rats were assigned to control and five treatment groups: PIH; PIH + vitamin B12; PIH + folic acid; PIH + Omega-3 fatty acids and PIH + combined micronutrient supplementation (vitamin B12 + folic acid + omega-3 fatty acids). L-Nitroarginine methylester (L-NAME; 50 mg/kg body weight/day) was used to induce hypertension during pregnancy. Blood Pressure (BP) was recorded during pregnancy and dams were dissected at d20 of gestation.ResultsAnimals from the PIH group demonstrated higher (p<0.01 for both) systolic and diastolic BP; lower (p<0.01) pup weight; higher dam plasma homocysteine (p<0.05) and dam and offspring malondialdehyde (MDA) (p<0.01), lower (p<0.05) placental and offspring liver DHA and higher (p<0.01) tumor necrosis factor–alpha (TNF–ά) levels as compared to control. Individual micronutrient supplementation did not offer much benefit. In contrast, combined supplementation lowered systolic BP, homocysteine, MDA and placental TNF-ά levels in dams and liver MDA and protein carbonyl in the offspring as compared to PIH group.ConclusionKey constituents of one carbon cycle (folic acid, vitamin B12 and DHA) may play a role in reducing oxidative stress and inflammation in preeclampsia.

“…The present data demonstrate that CYC and DOX exposure increases MDA levels and SOD activity in fetal brain tissue while it decreased CAT enzymatic activity indicating oxidative damage, consistent with previous studies (Wessels et al 2011). However, the presence of QR resulted in enhancement of the antioxidant capacity of the fetal tissues and reduction of free radical induced oxidative damage, a result supported by previous work (Tanir et al 2005).…”

Section: Discussionsupporting

confidence: 82%

Effect of chemotherapy exposure prior to pregnancy on fetal brain tissue and the potential protective role of quercetin

et al. 2014

Cyclophosphamide (CYC) and doxorubicin (DOX) are among the most effective and widely used anticancer chemotherapeutic drugs. Potential chemopreventive and chemotherapeutic functions have recently been attributed to flavonoids. We hypothesized that Quercetin (QR) would protect against the toxic effects of chemotherapeutic agents applied prior to pregnancy. Rats were treated with the chemotherapeutic drugs CYC (27 mg/kg) and DOX (1.8 mg/kg) applied in a single intraperitoneal dose once every 3 weeks for 10 weeks. QR was administered at a dose of 10 mg/kg/day by oral gavage. 48 h following the experimental chemotherapy exposure, female rats were transferred to cages containing male rat for mating. Fetal brain tissues were removed from fetuses extracted by cesarean section on the 20th day of gestation for evaluation of antioxidant parameters. A significant increase in superoxide dismutase and malondialdehyde activity was observed in CYC and DOX treatment groups relative to the control group (p \ 0.05). Similarly, carnitine acylcarnitine translocase and Glutathione activity was significantly reduced in the CYC and DOX groups relative to the control group (p \ 0.05). Our results indicate that the use of chemotherapeutic drugs before pregnancy can result in oxidative damage to fetal brain tissue. Therefore, women who have been exposed to chemotherapy and may become pregnant should be treated with antioxidant compounds such as QR to reduce the risk of damage to fetal brain tissues.