Ozone has important impact on climate change, the radiative balance and atmospheric chemistry. The main aim of this study is to analyses spatial and temporal variation of total column ozone as well as the trend over Turkey with MERRA-2 Reanalysis for the period 1980-2019. First of all, MERRA-2 total column ozone is compared with ground-based Brewer Spectrophotometer located in Ankara. It is found that MERRA-2 total column ozone agrees quite well (R2=0.9781) with that from the Brewer Spectrophotometer. Secondly, analysis are carried out with MERRA-2 total column ozone. The trend analysis over Turkey indicates statistically significant decreasing trend in the 1980-1999 period, non-significant decreasing trend in the 2000-2009 period and non-significant increasing trend in the 2010-2019 period. The highest annual average total column ozone are found in Istanbul, Canakkale and Samsun cities with the values of 326.3±6.9 DU, 324.0±6.7 DU and 322.2±6.6 DU, respectively. Total column ozone are the highest in spring, followed by winter, and the lowest in summer and autumn seasons over Turkey.